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. 1967 Aug;13(2):159–166.

Stimulation of lymphocyte transformation by tissue homogenates in recurrent oral ulceration

Thomas Lehner
PMCID: PMC1409313  PMID: 4952102

Abstract

Cultures were set up of white cells from patients with oral ulceration and from control subjects. The effects of saline extracts of foetal oral mucosa, liver, skin, colon, striated muscle and salivary gland on DNA synthesis by lymphocytes in the cultures were determined by measuring their uptake of [14C] thymidine. The results showed stimulation by oral mucosa, and to a lesser degree by skin, almost exclusively in patients with recurrent aphthous ulcers; colon and salivary gland stimulated the lymphocytes of occasional individuals regardless of the type or presence of oral ulceration, and liver produced a similar effect, but stimulated lymphocytes in a higher proportion of patients with aphthous ulcers. Specific stimulation by oral mucosa was increased during phases of ulceration and diminished during remissions.

It is suggested that lymphocyte transformation (indicated by [14C]thymidine uptake) in patients with recurrent aphthous ulceration is the result of specific antigenic stimulation of a portion of the lymphocyte population following previous encounter with an oral mucosal antigen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BACH F., HIRSCHHORN K. GAMMA-GLOBULIN PRODUCTION BY HUMAN LYMPHOCYTES IN VITRO. Exp Cell Res. 1963 Dec;32:592–595. doi: 10.1016/0014-4827(63)90197-6. [DOI] [PubMed] [Google Scholar]
  2. BACH F., HIRSCHHORN K. LYMPHOCYTE INTERACTION: A POTENTIAL HISTOCOMPATIBILITY TEST IN VITRO. Science. 1964 Feb 21;143(3608):813–814. doi: 10.1126/science.143.3608.813. [DOI] [PubMed] [Google Scholar]
  3. BAIN B., VAS M. R., LOWENSTEIN L. THE DEVELOPMENT OF LARGE IMMATURE MONONUCLEAR CELLS IN MIXED LEUKOCYTE CULTURES. Blood. 1964 Jan;23:108–116. [PubMed] [Google Scholar]
  4. BENDER M. A., PRESCOTT D. M. DNA synthesis and mitosis in cultures of human peripheral leukocytes. Exp Cell Res. 1962 Aug;27:221–229. doi: 10.1016/0014-4827(62)90225-2. [DOI] [PubMed] [Google Scholar]
  5. COWLING D. C., QUAGLINO D. EFFECT OF SOME ANTIGENS ON LEUCOCYTE CULTURES. J Pathol Bacteriol. 1965 Jan;89:63–70. doi: 10.1002/path.1700890108. [DOI] [PubMed] [Google Scholar]
  6. Caron G. A., Sarkany I. Lymphoblast transformation in sulphonamide sensitivity. Br J Dermatol. 1965 Nov;77(11):556–560. doi: 10.1111/j.1365-2133.1965.tb14575.x. [DOI] [PubMed] [Google Scholar]
  7. DUTTON R. W., EADY J. D. AN IN VITRO SYSTEM FOR THE STUDY OF THE MECHANISM OF ANTIGENIC STIMULATION IN THE SECONDARY RESPONSE. Immunology. 1964 Jan;7:40–53. [PMC free article] [PubMed] [Google Scholar]
  8. ELVES M. W., ISRAUELS M. C. LYMPHOCYTE TRANSFORMATION IN CULTURES OF MIXED LEUCOCYTES: A POSSIBLE TEST OF HISTOCOMPATIBILITY. Lancet. 1965 Jun 5;1(7397):1184–1186. doi: 10.1016/s0140-6736(65)92719-4. [DOI] [PubMed] [Google Scholar]
  9. FORBES I. J. SPECIFIC AND NON-SPECIFIC STIMULATION OF ANTIBODY SYNTHESIS BY HUMAN LEUCOCYTES IN VITRO. Lancet. 1965 Jan 23;1(7378):198–199. doi: 10.1016/s0140-6736(65)90979-7. [DOI] [PubMed] [Google Scholar]
  10. GOWANS J. L., McGREGOR D. D., COWEN D. M. Initiation of immune responses by small lymphocytes. Nature. 1962 Nov 17;196:651–655. doi: 10.1038/196651a0. [DOI] [PubMed] [Google Scholar]
  11. HASHEM N., HIRSCHHORN K., SEDLIS E., HOLT L. E., Jr Infantile eczema. Evidence of autoimmunity to human skin. Lancet. 1963 Aug 10;2(7302):269–270. doi: 10.1016/s0140-6736(63)90171-5. [DOI] [PubMed] [Google Scholar]
  12. HIRSCHHORN K., KOLODNY R. L., HASHEM N., BACH F. Mitogenic action of phytohaemagglutinin. Lancet. 1963 Aug 10;2(7302):305–306. doi: 10.1016/s0140-6736(63)90213-7. [DOI] [PubMed] [Google Scholar]
  13. HOLLAND P., MAUER A. M. DRUG-INDUCED IN-VITRO STIMULATION OF PERIPHERAL LYMPHOCYTES. Lancet. 1964 Jun 20;1(7347):1368–1369. doi: 10.1016/s0140-6736(64)92046-x. [DOI] [PubMed] [Google Scholar]
  14. JACKSON J. F. Leukocyte cultures in cytogenetics. Bull Tulane Univ Med Fac. 1962 May;21:103–105. [PubMed] [Google Scholar]
  15. KARUSH F., EISEN H. N. A theory of delayed hypersensitivity. Science. 1962 Jun 22;136(3521):1032–1039. doi: 10.1126/science.136.3521.1032. [DOI] [PubMed] [Google Scholar]
  16. LANDY M., SANDERSON R. P., BERNSTEIN M. T., JACKSON A. L. ANTIBODY PRODUCTION BY LEUCOCYTES IN PERIPHERAL BLOOD. Nature. 1964 Dec 26;204:1320–1321. doi: 10.1038/2041320a0. [DOI] [PubMed] [Google Scholar]
  17. LEHNER T. RECURRENT APHTHOUS ULCERATION AND AUTOIMMUNITY. Lancet. 1964 Nov 28;2(7370):1154–1155. doi: 10.1016/s0140-6736(64)92673-x. [DOI] [PubMed] [Google Scholar]
  18. LING N. R., HUSBAND E. M. SPECIFIC AND NON-SPECIFIC STIMULATION OF PERIPHERAL LYMPHOCYTES. Lancet. 1964 Feb 15;1(7329):363–365. doi: 10.1016/s0140-6736(64)92102-6. [DOI] [PubMed] [Google Scholar]
  19. LYCETTE R. R., PEARMAIN G. E. FURTHER OBSERVATIONS ON ANTIGEN-INDUCED MITOSIS. Lancet. 1963 Aug 24;2(7304):386–386. doi: 10.1016/s0140-6736(63)93063-0. [DOI] [PubMed] [Google Scholar]
  20. Lehner T. Behçet's syndrome and autoimmunity. Br Med J. 1967 Feb 25;1(5538):465–467. doi: 10.1136/bmj.1.5538.465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. MOORHEAD P. S., NOWELL P. C., MELLMAN W. J., BATTIPS D. M., HUNGERFORD D. A. Chromosome preparations of leukocytes cultured from human peripheral blood. Exp Cell Res. 1960 Sep;20:613–616. doi: 10.1016/0014-4827(60)90138-5. [DOI] [PubMed] [Google Scholar]
  22. MOYNIHAN P. C., JACKSON J. F., HARDY J. D. LYMPHOCYTE TRANSFORMATION AS AN IN-VITRO HISTOCOMPATIBILITY TEST. Lancet. 1965 Feb 27;1(7383):453–455. doi: 10.1016/s0140-6736(65)91587-4. [DOI] [PubMed] [Google Scholar]
  23. Maclaurin B. P. Homograft interaction in the test-tube. Lancet. 1965 Oct 23;2(7417):816–821. doi: 10.1016/s0140-6736(65)92447-5. [DOI] [PubMed] [Google Scholar]
  24. NOWELL P. C. Phytohemagglutinin: an initiator of mitosis in cultures of normal human leukocytes. Cancer Res. 1960 May;20:462–466. [PubMed] [Google Scholar]
  25. PATERSON P. Y. Transfer of allergic encephalomyelitis in rats by means of lymph node cells. J Exp Med. 1960 Jan 1;111:119–136. doi: 10.1084/jem.111.1.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. PEARMAIN G., LYCETTE R. R., FITZGERALD P. H. Tuberculin-induced mitosis in peripheral blood leucocytes. Lancet. 1963 Mar 23;1(7282):637–638. doi: 10.1016/s0140-6736(63)91275-3. [DOI] [PubMed] [Google Scholar]
  27. PULVERTAFT R. J., DONIACH D., ROITT I. M., HUDSON R. V. Cytotoxic effects of Hashimoto serum on human thyroid cells in tissue culture. Lancet. 1959 Aug 29;2(7096):214–216. doi: 10.1016/s0140-6736(59)90161-8. [DOI] [PubMed] [Google Scholar]
  28. SCHREK R. Cell transformations and mitoses produced in vitro by tuberculin purified protein derivative in human blood cells. Am Rev Respir Dis. 1963 May;87:734–738. doi: 10.1164/arrd.1963.87.5.734. [DOI] [PubMed] [Google Scholar]
  29. SELL S., GELL P. G. STUDIES ON RABBIT LYMPHOCYTES IN VITRO. I. STIMULATION OF BLAST TRANSFORMATION WITH AN ANTIALLOTYPE SERUM. J Exp Med. 1965 Aug 1;122:423–440. doi: 10.1084/jem.122.2.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Trayanova T. G., Sura V. V., Svet-Moldavsky G. J. Destruction of human cells in tissue culture by lymphocytes from patients with systemic lupus erythematosus. Lancet. 1966 Feb 26;1(7435):452–454. doi: 10.1016/s0140-6736(66)91456-5. [DOI] [PubMed] [Google Scholar]

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