Abstract
Mice given a single subcutaneous inoculation of highly-purified ovalbumin in water-in-oil emulsion yielded peak antibody titres which were about 500 times those stimulated by the same dose of ovalbumin without adjuvant, and which remained at the peak level for at least a year. Neither the oil, nor the emulsifier, nor the emulsion itself when injected at a separate site from the antigen, stimulated a response of this type. The response was not affected by a simultaneous subcutaneous injection of anti-ovalbumin, or by the later inoculation of a different antigen in water-in-oil emulsion, or by further inoculations of ovalbumin in saline.
Ovalbumin was detectable in emulsion recovered from injected mice for up to 544 days after inoculation and its half-life within the emulsion was found to be about 90 days.
The observations indicated that this type of adjuvant may exert its effect solely by the slow and even release of very tiny quantities of antigen over a long period of time. A mechanism by which such a release may take place is suggested.
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- BERLIN B. S. Tests for biologic safety of arlacel A. Ann Allergy. 1962 Jul;20:472–479. [PubMed] [Google Scholar]
- BOYDEN S. V. The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J Exp Med. 1951 Feb;93(2):107–120. doi: 10.1084/jem.93.2.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DALE M. M. The effect of the components of adjuvant emulsions on lymphatics in the mouse's ear. Br J Exp Pathol. 1961 Aug;42:297–302. [PMC free article] [PubMed] [Google Scholar]
- DUNN T. B. Normal and pathologic anatomy of the reticular tissue in laboratory mice, with a classification and discussion of neoplasms. J Natl Cancer Inst. 1954 Jun;14(6):1281–1433. [PubMed] [Google Scholar]
- FINKELSTEIN M. S., UHR J. W. SPECIFIC INHIBITION OF ANTIBODY FORMATION BY PASSIVELY ADMINISTERED 19S AND 7S ANTIBODY. Science. 1964 Oct 2;146(3640):67–69. doi: 10.1126/science.146.3640.67. [DOI] [PubMed] [Google Scholar]
- FREUND J., LIPTON M. M. Experimental allergic encephalomyelitis after the excision of the injection site of antigen-adjuvant emulsion. J Immunol. 1955 Dec;75(6):454–459. [PubMed] [Google Scholar]
- FREUND J., SCHRYVER E. M., MCGUINESS M. B., GEITNER M. B. Diphtheria antitoxin formation in the horse at site of injection of toxoid and adjuvants. Proc Soc Exp Biol Med. 1952 Dec;81(3):657–658. doi: 10.3181/00379727-81-19976. [DOI] [PubMed] [Google Scholar]
- Herbert W. J. Antigenicity of soluble protein in the presence of high levels of antibody: a possible mode of action of the antigen adjuvants. Nature. 1966 May 14;210(5037):747–748. doi: 10.1038/210747a0. [DOI] [PubMed] [Google Scholar]
- Herbert W. J. The preparation of antigenically defined conalbumin-free ovalbumin, by ion-exchange chromatography. Immunochemistry. 1967 May;4(3):143–150. doi: 10.1016/0019-2791(67)90123-1. [DOI] [PubMed] [Google Scholar]
- MCKINNEY R. W., DAVENPORT F. M. Studies on the mechanism of action of emulsified vaccines. J Immunol. 1961 Jan;86:91–100. [PubMed] [Google Scholar]
- STEINER J. W., LANGER B., SCHATZ D. L. The local and systemic effects of Freund's adjuvant and its fractions. Arch Pathol. 1960 Oct;70:424–434. [PubMed] [Google Scholar]
- TALMAGE D. W., DIXON F. J. The influence of adjuvants on the elimination of soluble protein antigens and the associated antibody responses. J Infect Dis. 1953 Sep-Oct;93(2):176–180. doi: 10.1093/infdis/93.2.176. [DOI] [PubMed] [Google Scholar]
- WEIGLE W. O., DIXON F. J., DEICHMILLER M. P. Effect of mycobacteria on antibody production induced by water-in-oil emulsions of soluble protein antigens. Proc Soc Exp Biol Med. 1960 Dec;105:535–541. doi: 10.3181/00379727-105-26168. [DOI] [PubMed] [Google Scholar]