Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1968 Mar;14(3):445–451.

Identical allotypic markers of heavy polypeptide chains present in different immunoglobulin classes

B Pernis, G Torrigiani, Luisa Amante, A S Kelus, J J Cebra
PMCID: PMC1409341  PMID: 4170514

Abstract

Purified IgA molecules, prepared from rabbit colostrum, have the same allotypic markers of the heavy (H) polypeptide chain as are present in the serum IgG of the same individual. When the dimeric molecule of rabbit colostral IgA is dissociated into its sub-units by reduction, its capacity to absorb an appropriate anti-allotypic antibody is quantitatively identical with that of the serum IgG of the same animal. It is concluded that there are neither qualitative nor quantitative differences in the antigenic expression of the allotypic determinants of the a locus present on the rabbit γ and α chains.

Full text

PDF
445

Images in this article

446-1FIG. 1
on p.446-1
446-1FIG. 2
on p.446-1
446-2FIG. 3
on p.446-2
446-2FIG. 4
on p.446-2

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Askonas B. A., Williamson A. R. Biosynthesis of immunoglobulins. Free light chain as an intermediate in the assembly of gamma G-molecules. Nature. 1966 Jul 23;211(5047):369–372. doi: 10.1038/211369a0. [DOI] [PubMed] [Google Scholar]
  2. Cebra J. J., Robbins J. B. Gamma-A-immunoglobulin from rabbit colostrum. J Immunol. 1966 Jul;97(1):12–24. [PubMed] [Google Scholar]
  3. Cebra J. J., Small P. A., Jr Polypeptide chain structure of rabbit immunoglobulins. 3. Secretory gamma-A-immunoglobulin from colostrum. Biochemistry. 1967 Feb;6(2):503–512. doi: 10.1021/bi00854a019. [DOI] [PubMed] [Google Scholar]
  4. Cohen S., Milstein C. Structure of antibody molecules. Nature. 1967 Apr 29;214(5087):449–passim. doi: 10.1038/214449a0. [DOI] [PubMed] [Google Scholar]
  5. DRAY S., DUBISKI S., KELUS A., LENNOX E. S., OUDIN J. A notation for allotypy. Nature. 1962 Aug 25;195:785–786. doi: 10.1038/195785a0. [DOI] [PubMed] [Google Scholar]
  6. FEINSTEIN A. CHARACTER AND ALLOTYPY OF AN IMMUNE GLOBULIN IN RABBIT COLOSTRUM. Nature. 1963 Sep 21;199:1197–1199. doi: 10.1038/1991197b0. [DOI] [PubMed] [Google Scholar]
  7. Kelus A. S., Gell P. G. Immunoglobulin allotypes of experimental animals. Prog Allergy. 1967;11:141–184. [PubMed] [Google Scholar]
  8. Kilmartin J. V., Clegg J. B. Amino-acid replacements in horse haemoglobin. Nature. 1967 Jan 21;213(5073):269–271. doi: 10.1038/213269a0. [DOI] [PubMed] [Google Scholar]
  9. Lichter E. A. Rabbit gamma A- and gamma M-immunoglobulins with allotypic specificities controlled by the a locus. J Immunol. 1967 Jan;98(1):139–142. [PubMed] [Google Scholar]
  10. Mårtensson L. Genes and immunoglobulins. Vox Sang. 1966 Sep-Oct;11(5):521–545. doi: 10.1111/j.1423-0410.1966.tb04251.x. [DOI] [PubMed] [Google Scholar]
  11. OUDIN J. Allotypy of rabbit serum proteins. I. Immuno-chemical analysis leading to the individualization of seven main allotypes. J Exp Med. 1960 Jul 1;112:107–124. doi: 10.1084/jem.112.1.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. OUDIN J. L'allotypie de certains antigènes protéidiques du sérum. C R Hebd Seances Acad Sci. 1956 May 23;242(21):2606–2608. [PubMed] [Google Scholar]
  13. OUDIN J. Réaction de précipitation spécifique entre des sérums d'animaux de même espèce. C R Hebd Seances Acad Sci. 1956 May 14;242(20):2489–2490. [PubMed] [Google Scholar]
  14. Pink J. R., Milstein C. Inter heavy-light chain disulphide bridge in immune globulins. Nature. 1967 Apr 1;214(5083):92–94. doi: 10.1038/214092a0. [DOI] [PubMed] [Google Scholar]
  15. Potter M., Appella E., Geisser S. Variations in the heavy polypeptide chain structure of gamma myeloma immunoglobulins from an inbred strain of mice and a hypothesis as to their origin. J Mol Biol. 1965 Dec;14(2):361–372. doi: 10.1016/s0022-2836(65)80187-5. [DOI] [PubMed] [Google Scholar]
  16. Press E. M., Piggot P. J., Porter R. R. The N- and c-terminal amino acid sequences of the heavy chain from a pathological human immunoglobulin IgG. Biochem J. 1966 May;99(2):356–366. doi: 10.1042/bj0990356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. SCHARFF M. D., UHR J. W. FUNCTIONAL RIBOSOMAL UNIT OF GAMMA-GLOBULIN SYNTHESIS. Science. 1965 Apr 30;148(3670):646–648. doi: 10.1126/science.148.3670.646. [DOI] [PubMed] [Google Scholar]
  18. Sell S. Isolation and characterization of rabbit colostral IgA. Immunochemistry. 1967 Jan;4(1):49–55. doi: 10.1016/0019-2791(67)90196-6. [DOI] [PubMed] [Google Scholar]
  19. Singer S. J., Doolittle R. F. Antibody active sites and immunoglobulin molecules. Science. 1966 Jul 1;153(3731):13–25. doi: 10.1126/science.153.3731.13. [DOI] [PubMed] [Google Scholar]
  20. Small P. A., Jr, Reisfeld R. A., Dray S. Peptide maps of rabbit gamma-G-immunoglobulin heavy chains controlled by allelic genes. J Mol Biol. 1966 Apr;16(2):328–333. doi: 10.1016/s0022-2836(66)80176-6. [DOI] [PubMed] [Google Scholar]
  21. South M. A., Cooper M. D., Wollheim F. A., Hong R., Good R. A. The IgA system. I. Studies of the transport and immunochemistry of IgA in the saliva. J Exp Med. 1966 Apr 1;123(4):615–627. doi: 10.1084/jem.123.4.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Stemke G. W., Fischer R. J. Rabbit 19S antibodies with allotypic specificities of the a-locus group. Science. 1965 Dec 3;150(3701):1298–1303. doi: 10.1126/science.150.3701.1298. [DOI] [PubMed] [Google Scholar]
  23. TODD C. W. Allotypy in rabbit 19S protein. Biochem Biophys Res Commun. 1963 May 3;11:170–175. doi: 10.1016/0006-291x(63)90329-2. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES