Abstract
Protein synthesis by human salivary glands was studied in vitro by incorporation of 14C-labelled amino acids and autoradiographs of double diffusion tests and immunoelectrophoresis of culture fluids.
Submandibular and parotid glands synthesized five serum proteins: one α1-globulin, one β1-globulin and three immunoglobulins, IgG, IgA and IgM. IgG and IgM, synthesized in small amounts, corresponded immunologically to serum IgG and IgM.
IgA, synthesized in large amounts, possessed antigenic determinants supplementary to serum IgA. There was also a synthesis of the free transport piece. Synthesis of serum IgA was never detected, alone or simultaneously with salivary IgA. These facts indicate a transport piece synthesis greater than local salivary IgA synthesis.
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Selected References
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- CHODIRKER W. B., TOMASI T. B., Jr GAMMA-GLOBULINS: QUANTITATIVE RELATIONSHIPS IN HUMAN SERUM AND NONVASCULAR FLUIDS. Science. 1963 Nov 22;142(3595):1080–1081. doi: 10.1126/science.142.3595.1080. [DOI] [PubMed] [Google Scholar]
- Cebra J. J., Robbins J. B. Gamma-A-immunoglobulin from rabbit colostrum. J Immunol. 1966 Jul;97(1):12–24. [PubMed] [Google Scholar]
- EAGLE H. Nutrition needs of mammalian cells in tissue culture. Science. 1955 Sep 16;122(3168):501–514. doi: 10.1126/science.122.3168.501. [DOI] [PubMed] [Google Scholar]
- ELLISON S. A., MASHIMO P. A., MANDEL I. D. Immunochemical studies of human saliva. I. The demonstration of serum proteins in whole and parotid saliva. J Dent Res. 1960 Sep-Oct;39:892–898. doi: 10.1177/00220345600390052301. [DOI] [PubMed] [Google Scholar]
- Haworth J. C., Dilling L. Concentration of gamma-A-globulin in serum, saliva, and nasopharyngeal secretions of infants and children. J Lab Clin Med. 1966 Jun;67(6):922–933. [PubMed] [Google Scholar]
- Hong R., Pollara B., Good R. A. A model for colostral IgA. Proc Natl Acad Sci U S A. 1966 Aug;56(2):602–607. doi: 10.1073/pnas.56.2.602. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Masson P. L., Carbonara A. O., Heremans J. F. Studies on the proteins of human saliva. Biochim Biophys Acta. 1965 Oct 18;107(3):485–500. doi: 10.1016/0304-4165(65)90192-3. [DOI] [PubMed] [Google Scholar]
- NEUMAN R. E., McCOY T. A. Growth-promoting properties of pyruvate oxal-acetate, and alpha-ketoglutarate for isolated Walker carcinosarcoma 256 cells. Proc Soc Exp Biol Med. 1958 Jun;98(2):303–306. doi: 10.3181/00379727-98-24025. [DOI] [PubMed] [Google Scholar]
- SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
- STOFFER H. R., KRAUS F. W., HOLMES A. C. Immunochemical identification of salivary proteins. Proc Soc Exp Biol Med. 1962 Nov;111:467–471. doi: 10.3181/00379727-111-27825. [DOI] [PubMed] [Google Scholar]
- South M. A., Cooper M. D., Wollheim F. A., Hong R., Good R. A. The IgA system. I. Studies of the transport and immunochemistry of IgA in the saliva. J Exp Med. 1966 Apr 1;123(4):615–627. doi: 10.1084/jem.123.4.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TOMASI T. B., Jr, TAN E. M., SOLOMON A., PRENDERGAST R. A. CHARACTERISTICS OF AN IMMUNE SYSTEM COMMON TO CERTAIN EXTERNAL SECRETIONS. J Exp Med. 1965 Jan 1;121:101–124. doi: 10.1084/jem.121.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TOMASI T. B., Jr, ZIGELBAUM S. THE SELECTIVE OCCURENCE OF GAMMA-1A GLOBULINS IN CERTAIN BODY FLUIDS. J Clin Invest. 1963 Oct;42:1552–1560. doi: 10.1172/JCI104840. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Furth R., Schuit R. E., Hijmans W. The formation of immunoglobulins by human tissues in vitro. I. The methods and their specificity. Immunology. 1966 Jul;11(1):1–11. [PMC free article] [PubMed] [Google Scholar]
- van Furth R. The formation of immunoglobulins by human tissues in vitro. II. Quantitative studies. Immunology. 1966 Jul;11(1):13–18. [PMC free article] [PubMed] [Google Scholar]