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. 1968 Apr;14(4):511–525.

Antigen uptake in vivo by peritoneal macrophages from normal mice, and those undergoing primary or secondary responses

Joan M Rhodes, Inga Lind
PMCID: PMC1409386  PMID: 4871348

Abstract

The in vivo uptake of human serum albumin and ferritin by peritoneal macrophages from normal, primary and secondary response mice has been studied. Both antigens were ingested by the macrophages irrespective of whether they were injected simultaneously or sequentially. Less than 1 per cent of the cells ingested only one antigen.

There was no indication that one antigen was ingested in preference to the other in any of the groups of mice.

The significance of these findings for the immune response is discussed.

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Selected References

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  1. Amkraut A. A., Garvey J. S., Campbell D. H. Competition of haptens. J Exp Med. 1966 Sep 1;124(3):293–306. doi: 10.1084/jem.124.3.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BOYDEN S. V., SORKIN E. The adsorption of antigen by spleen cells previously treated with antiserum in vitro. Immunology. 1960 Jul;3:272–283. [PMC free article] [PubMed] [Google Scholar]
  3. Ben-Efraim S., Liacopoulos P. Compétition entre stéréoisomères D et L d'antigènes synthétiques au cours de l'induction de l'hypersensibilité retardée. C R Acad Sci Hebd Seances Acad Sci D. 1965 Nov 29;261(22):4918–4921. [PubMed] [Google Scholar]
  4. Berken A., Benacerraf B. Properties of antibodies cytophilic for macrophages. J Exp Med. 1966 Jan 1;123(1):119–144. doi: 10.1084/jem.123.1.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cebra J. J., Goldstein G. Chromatographic purification of tetramethylrhodamine-immune globulin conjugates and their use in the cellular localization of rabbit gamma-globulin polypeptide chains. J Immunol. 1965 Aug;95(2):230–245. [PubMed] [Google Scholar]
  6. Doré C. F., Balfour B. M. A device for preparing cell spreads. Immunology. 1965 Oct;9(4):403–405. [PMC free article] [PubMed] [Google Scholar]
  7. HALPERN B. N., BIOZZI G., BENACERRAF B., STIFFEL C. Phagocytosis of foreign red blood cells by the reticulo-endothelial system. Am J Physiol. 1957 Jun;189(3):520–526. doi: 10.1152/ajplegacy.1957.189.3.520. [DOI] [PubMed] [Google Scholar]
  8. LITT M. STUDIES IN EXPERIMENTAL EOSINOPHILIA. VI. UPTAKE OF IMMUNE COMPLEXES BY EOSINOPHILS. J Cell Biol. 1964 Nov;23:355–361. doi: 10.1083/jcb.23.2.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lee A., Cooper G. N. Adhesion of homologous and heterologous red cells to peritoneal macrophages. Aust J Exp Biol Med Sci. 1966 Oct;44(5):527–538. doi: 10.1038/icb.1966.48. [DOI] [PubMed] [Google Scholar]
  10. MARSHALL J. D., EVELAND W. C., SMITH C. W. Superiority of fluorescein isothiocyanate (Riggs) for fluorescent-antibody technic with a modification of its application. Proc Soc Exp Biol Med. 1958 Aug-Sep;98(4):898–900. doi: 10.3181/00379727-98-24222. [DOI] [PubMed] [Google Scholar]
  11. Nelson D. S., Boyden S. V. Macrophage cytophilic antibodies and delayed hypersensitivity. Br Med Bull. 1967 Jan;23(1):15–20. doi: 10.1093/oxfordjournals.bmb.a070508. [DOI] [PubMed] [Google Scholar]
  12. PERKINS E. H., LEONARD M. R. Specificity of phagocytosis as it may relate to antibody formation. J Immunol. 1963 Feb;90:228–237. [PubMed] [Google Scholar]
  13. RHODES J. M. IN VITRO STUDIES ON THE FATE OF ANTIGEN. IV. THE FATE OF SOLUBLE AND PRECIPITATED ANTIGEN-ANTIBODY COMPLEXES IN THE PRESENCE OF GUINEA PIG EXUDATE CELLS, CORRELATED WITH THEIR SKIN REACTIVITY. Int Arch Allergy Appl Immunol. 1964;25:225–241. [PubMed] [Google Scholar]
  14. Schechter I., Sela M. Preferential immune response to D-amino acid residues in poly-DL-amino acid determinants. Biochim Biophys Acta. 1965 Jun 15;104(1):301–302. doi: 10.1016/0304-4165(65)90253-9. [DOI] [PubMed] [Google Scholar]
  15. TALMAGE D. W., DIXON F. J., BUKANTZ S. C., DAMMIN G. J. Antigen elimination from the blood as an early manifestation of the immune response. J Immunol. 1951 Oct;67(4):243–255. [PubMed] [Google Scholar]
  16. TOKUMARU T. A kinetic study on the labeling of serum globulin with fluorescein isothiocyanate by means of the gel filtration technique. J Immunol. 1962 Aug;89:195–203. [PubMed] [Google Scholar]
  17. UHR J. W., WEISSMAN G. INTRACELLULAR DISTRIBUTION AND DEGRADATION OF BACTERIOPHAGE IN MAMMALIAN TISSUES. J Immunol. 1965 Apr;94:544–550. [PubMed] [Google Scholar]

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