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. 1968 May;14(5):601–609.

Changes in the proportion of guinea-pig γ1 and γ2 antibodies during immunization and the cellular localization of these immunoglobulins

V Nussenzweig, I Green, P Vassalli, B Benacerraf
PMCID: PMC1409405  PMID: 4872344

Abstract

Guinea-pigs immunized with DNP—BSA first produced anti-DNP antibodies of the γ2-class followed by anti-DNP antibodies of the γ1-class. The cellular localization of γ2 and γ1 immunoglobulins was investigated by immunofluorescence techniques during this shifting period. The γ2- and γ1-classes of immunoglobulins were, with few possible exceptions, produced in separate cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENACERRAF B., BIOZZI G., HALPERN B. N., MOUTON D., STIFFEL C. Influence de la quantité d'iode fixee sur les proteines seriques normales et modifiees par la chaleur sur la phagocytose de ces colloïdes par les cellules du S.R.E. Ann Inst Pasteur (Paris) 1957 Jan;92(1):89–111. [PubMed] [Google Scholar]
  2. BENACERRAF B., LEVINE B. B. Immunological specificity of delayed and immediate hypersensitivity reactions. J Exp Med. 1962 May 1;115:1023–1036. doi: 10.1084/jem.115.5.1023. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BENACERRAF B., OVARY Z., BLOCH K. J., FRANKLIN E. C. Properties of guinea pig 7S antibodies. I. Electrophoretic separation of two types of guinea pig 7S antibodies. J Exp Med. 1963 Jun 1;117:937–949. doi: 10.1084/jem.117.6.937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BERNIER G. M., CEBRA J. J. POLYPEPTIDE CHAINS OF HUMAN GAMMA-GLOBULIN: CELLULAR LOCALIZATION BY FLUORESCENT ANTIBODY. Science. 1964 Jun 26;144(3626):1590–1591. doi: 10.1126/science.144.3626.1590. [DOI] [PubMed] [Google Scholar]
  5. Bernier G. M., Cebra J. J. Frequency distribution of alpha, gamma, kappa and lambda polypeptide chains in human lymphoid tissues. J Immunol. 1965 Aug;95(2):246–253. [PubMed] [Google Scholar]
  6. Burtin P., Buffe D. Etude de l'origine cellulaire des immunoglobulines humaines et de leurs chaines polypeptidiques. Rev Fr Etud Clin Biol. 1966 Aug-Sep;11(7):687–695. [PubMed] [Google Scholar]
  7. Campbell D. H., Luescher E., Lerman L. S. Immunologic Adsorbents: I. Isolation of Antibody by Means of a Cellulose-Protein Antigen. Proc Natl Acad Sci U S A. 1951 Sep;37(9):575–578. doi: 10.1073/pnas.37.9.575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cebra J. J., Colberg J. E., Dray S. Rabbit lymphoid cells differentiated with respect to alpha-, gamma-, and mu- heavy polypeptide chains and to allotypic markers Aa1 and Aa2. J Exp Med. 1966 Mar 1;123(3):547–558. doi: 10.1084/jem.123.3.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. EISEN H. N. PREPARATION OF PURIFIED ANTI-2,4-DINITROPHENYL ANTIBODIES. Methods Med Res. 1964;10:94–102. [PubMed] [Google Scholar]
  10. Green I., Vassalli P., Nussenzweig V., Benacerraf B. Specificity of the antibodies produced by single cells following immunization with antigens bearing two types of antigenic determinants. J Exp Med. 1967 Mar 1;125(3):511–526. doi: 10.1084/jem.125.3.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MELLORS R. C., KORNGOLD L. THE CELLULAR ORIGIN OF HUMAN IMMUNOGLOBULINS (GAMMA-2, GAMMA-1M, GAMMA-1A). J Exp Med. 1963 Sep 1;118:387–396. doi: 10.1084/jem.118.3.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. NISONOFF A. ENZYMATIC DIGESTION OF RABBIT GAMMA GLOBULIN AND ANTIBODY AND CHROMATOGRAPHY OF DIGESTION PRODUCTS. Methods Med Res. 1964;10:134–141. [PubMed] [Google Scholar]
  13. NOSSAL G. J., SZENBERG A., ADA G. L., AUSTIN C. M. SINGLE CELL STUDIES ON 19S ANTIBODY PRODUCTION. J Exp Med. 1964 Mar 1;119:485–502. doi: 10.1084/jem.119.3.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. NUSSENZWEIG V., BENACERRAF B. STUDIES ON THE PROPERTIES OF FRAGMENTS OF GUINEA PIG GAMMA-1 AND GAMMA-2 ANTIBODIES OBTAINED BY PAPAIN DIGESTION AND MILD REDUCTION. J Immunol. 1964 Dec;93:1008–1014. [PubMed] [Google Scholar]
  15. Nussenzweig V., Benacerraf B. Presence of identical antigenic determinants in the Fd fragments of gamma-1- and gamma-2-guinea pig immunoglobulins. J Immunol. 1966 Aug;97(2):171–176. [PubMed] [Google Scholar]
  16. Nussenzweig V., Lamm M. E., Benacerraf B. Presence of two types of L polypeptide chains in guinea pig 7S immunoglobulins. J Exp Med. 1966 Nov 1;124(5):787–803. doi: 10.1084/jem.124.5.787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. OETTGEN H. F., BINAGHI R. A., BENACERRAF B. HEXOSE CONTENT OF GUINEA PIG GAMMA-1 AND GAMMA-2 IMMUNOGLOBULINS. Proc Soc Exp Biol Med. 1965 Feb;118:336–342. [PubMed] [Google Scholar]
  18. PERNIS B., CHIAPPINO G. IDENTIFICATION IN HUMAN LYMPHOID TISSUES OF CELLS THAT PRODUCE GROUP 1 OR GROUP 2 GAMMA-GLOBULINS. Immunology. 1964 Sep;7:500–506. [PMC free article] [PubMed] [Google Scholar]
  19. PORTER R. R. The hydrolysis of rabbit y-globulin and antibodies with crystalline papain. Biochem J. 1959 Sep;73:119–126. doi: 10.1042/bj0730119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. WHITE R. G., JENKINS G. C., WILKINSON P. C. The production of skin-sensitizing antibody in the guinea-pig. Int Arch Allergy Appl Immunol. 1963;22:156–165. doi: 10.1159/000229362. [DOI] [PubMed] [Google Scholar]

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