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. 1968 Aug;15(2):319–321.

The adherence to Salmonella enteritidis of IgG, IgM and β1C-globulins from guinea-pig serum

K D Beernink, J P Steward
PMCID: PMC1409449  PMID: 5673288

Abstract

The adherence of globulins from guinea-pig serum to Salmonella enteritidis was determined when these bacteria were sensitized in vitro: (a) in serum from normal guinea-pigs lacking detectable bactericidal antibody, (b) in serum from normal guinea-pigs possessing low titre of bactericidal antibody, and (c) in serum from guinea-pigs actively immunized against this Salmonella. Sensitized bacteria were washed free of contaminating serum constituents and used to immunize rabbits. Guinea-pig globulins that adhered to the bacteria and to which the rabbit responded by the production of antibody were identified by immunoelectrophoresis. IgM, but no IgG, attached to bacteria sensitized in serum lacking bactericidins. Both IgG and IgM attached to bacteria sensitized in immune serum or in serum containing `normal' bactericidins. β1C-globulin attached to bacteria in all three cases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beernink K. D., Steward J. P. Proteins adhering to Escherichia coli after exposure to guinea-pig serum. Immunology. 1967 Feb;12(2):207–218. [PMC free article] [PubMed] [Google Scholar]
  2. Bladen H. A., Gewurz H., Mergenhagen S. E. Interactions of the complement system with the surface and endotoxic lipopolysaccharide of Veillonella alcalescens. J Exp Med. 1967 May 1;125(5):767–786. doi: 10.1084/jem.125.5.767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GITLIN D., ROSEN F. S., MICHAEL J. G. Transient 19S gammaglobulin deficiency in the newborn infant, and its significance. Pediatrics. 1963 Feb;31:197–208. [PubMed] [Google Scholar]
  4. MICHAEL J. G., ROSEN F. S. ASSOCIATION OF "NATURAL" ANTIBODIES TO GRAM-NEGATIVE BACTERIA WITH THE GAMMA-1-MACROGLOBULINS. J Exp Med. 1963 Oct 1;118:619–626. doi: 10.1084/jem.118.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Spitznagel J. K. Normal serum cytotoxicity for P32-labeled smooth Enterobacteriaceae. 3. Isolation of a gammag normal antibody and characterization of other serum factors causing P32 loss. J Bacteriol. 1966 Jan;91(1):401–408. doi: 10.1128/jb.91.1.401-408.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Steward J. P., Collis L. R., Roantree R. J. Effects of immunization of guinea pigs lacking bactericidal antibody against Salmonella enteritidis. J Immunol. 1966 Aug;97(2):224–230. [PubMed] [Google Scholar]

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