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. 1969 Feb;16(2):175–185.

The immune reactivity of lymph node-derived spleen colonies and intercolonial cells

Harriet Gershon, M Feldman
PMCID: PMC1409583  PMID: 5769976

Abstract

Using a method for cloning lymph node (LN) derived cells in the spleens of lethally irradiated mice, it was determined that the macroscopic colonies observed were haemopoietic whereas the intercolonial cells were primarily lymphoid. The immune response detected in isolated colonies was shown to be attributable to contamination of the clones by immunologically reactive interclonal cells. It was also demonstrated that pre-treatment of LN donor mice with PHA enhanced cloning efficiency but was not a necessary procedure for obtaining LN-derived colonies in the spleens of irradiated recipient mice.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brenner S., Milstein C. Origin of antibody variation. Nature. 1966 Jul 16;211(5046):242–243. doi: 10.1038/211242a0. [DOI] [PubMed] [Google Scholar]
  2. Cohen S., Milstein C. Structure of antibody molecules. Nature. 1967 Apr 29;214(5087):449–passim. doi: 10.1038/214449a0. [DOI] [PubMed] [Google Scholar]
  3. Dreyer W. J., Bennett J. C. The molecular basis of antibody formation: a paradox. Proc Natl Acad Sci U S A. 1965 Sep;54(3):864–869. doi: 10.1073/pnas.54.3.864. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edelman G. M., Gally J. A. Somatic recombination of duplicated genes: an hypothesis on the origin of antibody diversity. Proc Natl Acad Sci U S A. 1967 Feb;57(2):353–358. doi: 10.1073/pnas.57.2.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FRIEDMAN H. DISTRIBUTION OF ANTIBODY PLAQUE FORMING CELLS IN VARIOUS TISSUES OF SEVERAL STRAINS OF MICE INJECTED WITH SHEEP ERYTHROCYTES. Proc Soc Exp Biol Med. 1964 Nov;117:526–530. doi: 10.3181/00379727-117-29628. [DOI] [PubMed] [Google Scholar]
  6. Feldman M., Mekori T. Antibody production by 'cloned' cell populations. Immunology. 1966 Feb;10(2):149–160. [PMC free article] [PubMed] [Google Scholar]
  7. HAUROWITZ F. ANTIBODY FORMATION AND THE CODING PROBLEM. Nature. 1965 Feb 27;205:847–851. doi: 10.1038/205847a0. [DOI] [PubMed] [Google Scholar]
  8. Kennedy J. C., Siminovitch L., Till J. E., McCulloch E. A. A transplantation assay for mouse cells responsive to antigenic stimulation by sheep erythrocytes. Proc Soc Exp Biol Med. 1965 Dec;120(3):868–873. doi: 10.3181/00379727-120-30678. [DOI] [PubMed] [Google Scholar]
  9. LEDERBERG J. Genes and antibodies. Science. 1959 Jun 19;129(3364):1649–1653. doi: 10.1126/science.129.3364.1649. [DOI] [PubMed] [Google Scholar]
  10. MEKORI T., FELDMAN M. PROTECTION OF X-IRRADIATED MICE BY "CLONED" HAEMOPOIETIC CELLS. Transplantation. 1965 Jan;3:98–113. doi: 10.1097/00007890-196501000-00012. [DOI] [PubMed] [Google Scholar]
  11. Mekori T., Chieco-Bianci L., Feldman M. Production of clones of lymphoid cell populations. Nature. 1965 Apr 24;206(982):367–368. doi: 10.1038/206367a0. [DOI] [PubMed] [Google Scholar]
  12. Micklem H. S. Effect of phytohemagglutinin-M (PHA) on the spleen-colony-forming capacity of mouse lymph node and blood cells. Transplantation. 1966 Nov;4(6):732–741. [PubMed] [Google Scholar]
  13. Playfair J. H., Papermaster B. W., Cole L. J. Focal antibody production by transferred spleen cells in irradiated mice. Science. 1965 Aug 27;149(3687):998–1000. doi: 10.1126/science.149.3687.998. [DOI] [PubMed] [Google Scholar]
  14. SIMONSEN M., ENGELBRETH-HOLM J., JENSEN E., POULSEN H. A study of the graft-versus-host reaction in transplantation to embryos, F1 hybrids, and irradiated animals. Ann N Y Acad Sci. 1958 Oct 7;73(3):834–841. doi: 10.1111/j.1749-6632.1959.tb40863.x. [DOI] [PubMed] [Google Scholar]
  15. Smithies O. Antibody variability. Somatic recombination between the elements of "antibody gene pairs" may explain antibody variability. Science. 1967 Jul 21;157(3786):267–273. doi: 10.1126/science.157.3786.267. [DOI] [PubMed] [Google Scholar]
  16. Szilard L. THE MOLECULAR BASIS OF ANTIBODY FORMATION. Proc Natl Acad Sci U S A. 1960 Mar;46(3):293–302. doi: 10.1073/pnas.46.3.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Whitehouse H. L. Cross over model of antibody variability. Nature. 1967 Jul 22;215(5099):371–374. doi: 10.1038/215371a0. [DOI] [PubMed] [Google Scholar]

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