Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1969 May;16(5):573–580.

The competitive effect of DNP—poly-L-lysine in responder and non-responder guinea-pigs

S Ben-Efraim, P Liacopoulos
PMCID: PMC1409600  PMID: 5787766

Abstract

The synthetic antigen DNP—p(Lys), which it is known to induce an immune response only in a certain percentage of randomly bred guinea-pigs, inhibited the immune response towards p(Tyr, Glu, Ala) and p(Tyr, Glu) synthetic antigens in all the guinea-pigs tested. The extent of the competitive effect was related to the quantity of DNP—p(Lys) injected.

The hapten moiety DNP—mono-lysine, had no inhibitory effect, while the p(Lys) carrier interfered with the response to p(Tyr, Glu), although to a lesser extent than did the complete conjugate.

Full text

PDF
573

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ben-Efraim S., Liacopoulos P. Inhibition, no-effect or enhancement of immune responses following injection of mixtures of immunogenic and non-immunogenic synthetic polypeptides. Immunology. 1967 May;12(5):517–524. [PMC free article] [PubMed] [Google Scholar]
  2. GILL T. J., 3rd, KUNZ H. W., GOULD H. J., DOTY P. STUDIES ON SYNTHETIC POLYPEPTIDE ANTIGENS. XI. THE ANTIGENICITY OF OPTICALLY ISOMERIC SYNTHETIC POLYPEPTIDES. J Biol Chem. 1964 Apr;239:1107–1113. [PubMed] [Google Scholar]
  3. Green I., Paul W. E., Benacerraf B. The behavior of hapten-poly-L-lysine conjugates as complete antigens in genetic responder and as haptens in nonresponder guinea pigs. J Exp Med. 1966 May 1;123(5):859–879. doi: 10.1084/jem.123.5.859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. KANTOR F. S., OJEDA A., BENCARERRAF B. Studies on artifical antigens. I. Antigenicity of DNP-polylysine and DNP copolymer of lysine and glutamic acid in guinea pigs. J Exp Med. 1963 Jan 1;117:55–69. doi: 10.1084/jem.117.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LEVINE B. B., OJEDA A., BENACERRAF B. BASIS FOR THE ANTIGENICITY OF HAPTEN-POLY-L-LYSINE CONJUGATES IN RANDOM-BRED GUINEA PIGS. Nature. 1963 Nov 9;200:544–546. doi: 10.1038/200544a0. [DOI] [PubMed] [Google Scholar]
  6. LEVINE B. B., OJEDA A., BENACERRAF B. STUDIES ON ARTIFICIAL ANTIGENS. III. THE GENETIC CONTROL OF THE IMMUNE RESPONSE TO HAPTEN-POLY-L-LYSINE CONJUGATES IN GUINEA PIGS. J Exp Med. 1963 Dec 1;118:953–957. doi: 10.1084/jem.118.6.953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Liacopoulos P., Ben-Efraim S., Harel S., Gille F. Selective immune reactivity to DNP-poly-L-lysine in randomly bred guinea-pigs. Immunology. 1969 May;16(5):581–588. [PMC free article] [PubMed] [Google Scholar]
  8. OVARY Z., BENACERRAF B., BLOCH K. J. Properties of guinea pig 7S antibodies. II. Identification of antibodies involved in passive cutaneous and systemic anaphylaxis. J Exp Med. 1963 Jun 1;117:951–964. doi: 10.1084/jem.117.6.951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SELA M., FUCHS S., ARNON R. Studies on the chemical basis of the antigenicity of proteins. 5. Synthesis, characterization and immunogenicity of some multichain and linear polypeptides containing tyrosine. Biochem J. 1962 Oct;85:223–235. doi: 10.1042/bj0850223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Stupp Y., Borek F., Sela M. Studies on the types of immune responses to synthetic antigens in guinea-pigs. Immunology. 1966 Dec;11(6):561–570. [PMC free article] [PubMed] [Google Scholar]
  11. WHITE R. G., JENKINS G. C., WILKINSON P. C. The production of skin-sensitizing antibody in the guinea-pig. Int Arch Allergy Appl Immunol. 1963;22:156–165. doi: 10.1159/000229362. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES