Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1969 Apr;16(4):473–480.

The effects of Cu-chlorophyllin on the active site formation of each component of guinea-pig complement

H Okada, K Nishioka, T Sindo
PMCID: PMC1409642  PMID: 5770389

Abstract

The effects of Cu-chlorophyllin on the formation of intermediate complexes (site formation) by each component of guinea-pig complement on sensitized sheep erythrocytes were examined. When the Z value of immune haemolysis varied between 0·30 and 2·66, the 50 per cent inhibition doses of Cu-chlorophyllin on C′1, C′2, C′5, C′6 and C′7 were 2·3–3·2×10-5 M, 0·31–0·37×10-5 M, 1·4–1·6×10-5 M, 1·0–1·2×10-5 M and 2·4–2·8×10-5 M, respectively. Site generation of C′3 and C′9 was enhanced whereas no effect was observed on C′4 and C′8 site formation.

Full text

PDF
473

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BECKER E. L. Concerning the mechanism of complement action. V. The early steps in immune hemolysis. J Immunol. 1960 Mar;84:299–308. [PubMed] [Google Scholar]
  2. Inoue K., Nelson R. A., Jr The isolation and characterization of a new component of hemolytic complement, C'3e. J Immunol. 1965 Aug;95(2):355–367. [PubMed] [Google Scholar]
  3. Inoue K., Nelson R. A., Jr The isolation and characterization of a ninth component of hemolytic complement, C'3f. J Immunol. 1966 Mar;96(3):386–400. [PubMed] [Google Scholar]
  4. NISHIOKA K., LINSCOTT W. D. COMPONENTS OF GUINEA PIG COMPLEMENT. I. SEPARATION OF A SERUM FRACTION ESSENTIAL FOR IMMUNE HEMOLYSIS AND IMMUNE ADHERENCE. J Exp Med. 1963 Nov 1;118:767–793. doi: 10.1084/jem.118.5.767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. NISHIOKA K. [COMPLEMENT AND ALLERGY]. Arerugi. 1964 May;13:285–298. [PubMed] [Google Scholar]
  6. Nelson R. A., Jr, Jensen J., Gigli I., Tamura N. Methods for the separation, purification and measurement of nine components of hemolytic complement in guinea-pig serum. Immunochemistry. 1966 Mar;3(2):111–135. doi: 10.1016/0019-2791(66)90292-8. [DOI] [PubMed] [Google Scholar]
  7. Sindo T., Haga K., Fujii G., Nishioka K. Studies on the inhibition of chlorophyllin derivatives against complement activities and anaphylactic reaction. Jpn J Exp Med. 1966 Oct;36(5):489–498. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES