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. 1977 Apr;18(4):259–265. doi: 10.1136/gut.18.4.259

Immunofluorescent localisation of enterokinase in human small intestine.

J Hermon-Taylor, J Perrin, D A Grant, A Appleyard, M Bubel, A I Magee
PMCID: PMC1411444  PMID: 324873

Abstract

The distribution of enterokinase in human intestine was studied in operative mucosal biopsies using specific antiserum to human enterokinase, previously purified to apparent homogeneity by affinity chromatography and immunoabsorption. Fluorescence was observed in the brush-border and glycocalyx of the duodenum and proximal 15 cm of jejunum distal to the D/J flexure. Distal jejunum and ileum as well as stomach and colon were consistently negative. Brunner's glands and goblet cells were never stained by specific antibody. Preliminary evidence was obtained that the human enterokinase molecule contains a specific antigenic determinant in its polypeptide component and a second determinant in the oligosaccharide moiety which cross-reacts with blood group A. Preliminary evidence was also obtained that mucosal synthesis of enterokinase may be impaired in jaundice due to carcinoma of the pancreas and induced in the small intestine distal to the normal limit of synthesis after pancreatico-duodenectomy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Eggermont E., Molla A. M., Rutgeerts L., Tytgat G. The source of human enterokinase. Lancet. 1971 Aug 14;2(7720):369–369. doi: 10.1016/s0140-6736(71)90079-1. [DOI] [PubMed] [Google Scholar]
  2. Fuks A., Banjo C., Shuster J., Freedman S. O., Gold P. Carcinoembryonic antigen (CEA): molecular biology and clinical significance. Biochim Biophys Acta. 1975 Jul 11;417(2):123–152. doi: 10.1016/0304-419x(75)90002-5. [DOI] [PubMed] [Google Scholar]
  3. GLYNN L. E., HOLBOROW E. J. [Distribution of blood-group substances in human tissues]. Br Med Bull. 1959 May;15(2):150–153. doi: 10.1093/oxfordjournals.bmb.a069740. [DOI] [PubMed] [Google Scholar]
  4. Gospodarowicz D. Single step purification of ovine luteinizing hormone by affinity chromatography. J Biol Chem. 1972 Oct 25;247(20):6491–6498. [PubMed] [Google Scholar]
  5. Grant D. A., Hermon-Taylor J. Purification of porcine enterokinase by affinity chromatography. Biochem J. 1975 May;147(2):363–366. doi: 10.1042/bj1470363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Grant D. A., Hermon-Taylor J. The purification of human enterokinase by affinity chromatography and immunoadsorption. Some observations on its molecular characteristics and comparisons with the pig enzyme. Biochem J. 1976 May 1;155(2):243–254. doi: 10.1042/bj1550243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hadorn B., Hess J., Troesch V., Verhaage W., Götze H., Bender S. W. Role of bile acids in the activation of trypsinogen by enterokinase: disturbance of trypsinogen activation in patients with intrahepatic biliary atresia. Gastroenterology. 1974 Apr;66(4):548–555. [PubMed] [Google Scholar]
  8. Hadorn B., Steiner N., Sumida C., Peters T. J. Intestinal enterokinase. Mechanisms of tts "secretion" into the lumen of the small intestine. Lancet. 1971 Jan 23;1(7691):165–166. doi: 10.1016/s0140-6736(71)91936-2. [DOI] [PubMed] [Google Scholar]
  9. Hadorn B., Tarlow M. J., Lloyd J. K., Wolff O. H. Intestinal enterokinase deficiency. Lancet. 1969 Apr 19;1(7599):812–813. doi: 10.1016/s0140-6736(69)92071-6. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Lebenthal E., Antonowicz I., Shwachman H. Enterokinase and trypsin activities in pancreatic insufficiency and diseases of the small intestine. Gastroenterology. 1976 Apr;70(4):508–512. [PubMed] [Google Scholar]
  12. Lobley R. W., Holmes R. The localization of enterokinase to the brush border membrane of the guinea pig small intestine. Gut. 1970 Dec;11(12):1059–1059. [PubMed] [Google Scholar]
  13. Lobley R. W., Moss S., Holmes R. Proceedings: Brush-border localization of human enterokinase. Gut. 1973 Oct;14(10):817–817. [PubMed] [Google Scholar]
  14. Lojda Z., Malis F. Histochemical demonstration of enterokinase. Histochemie. 1972;32(1):23–29. doi: 10.1007/BF00277468. [DOI] [PubMed] [Google Scholar]
  15. Louvard D., Maroux S., Baratti J., Desnuelle P. On the distribution of enterokinase in porcine intestine and on its subcellular localization. Biochim Biophys Acta. 1973 May 5;309(1):127–137. doi: 10.1016/0005-2744(73)90324-0. [DOI] [PubMed] [Google Scholar]
  16. März L., Bahl O. P., Mohn J. F. Blood-group activity of human chorionic gonadotropin. Biochem Biophys Res Commun. 1973 Dec 10;55(3):717–723. doi: 10.1016/0006-291x(73)91203-5. [DOI] [PubMed] [Google Scholar]
  17. Nordström C., Dahlqvist A. Localisation of human enterokinase. Lancet. 1972 Oct 28;2(7783):933–934. doi: 10.1016/s0140-6736(72)92584-6. [DOI] [PubMed] [Google Scholar]
  18. Nordström C., Dahlqvist A. Quantitative distribution of some enzymes along the villi and crypts of human small intestine. Scand J Gastroenterol. 1973;8(5):406–416. [PubMed] [Google Scholar]
  19. Nordström C., Dahlqvist A. Rat enterokinase: the effect of ions and the localization in the intestine. Biochim Biophys Acta. 1971 Jul 21;242(1):209–225. doi: 10.1016/0005-2744(71)90101-x. [DOI] [PubMed] [Google Scholar]
  20. Rutgeerts L., Tytgat G., Eggermont E., Gray G. M. Localization of enterokinase in man. Gastroenterology. 1972 Aug;63(2):381–382. [PubMed] [Google Scholar]
  21. Schmitz J., Preiser H., Maestracci D., Crane R. K., Troesch V., Hadorn B. Subcellular localization of enterokinase in human small intestine. Biochim Biophys Acta. 1974 May 24;343(3):435–439. doi: 10.1016/0304-4165(74)90260-8. [DOI] [PubMed] [Google Scholar]
  22. Schneider R., Troesch V., Hadorn B. On the cellular distribution of sucrase and enterokinase in different populations of rat intestinal epithelial cells isolated by a vibration method. Biol Gastroenterol (Paris) 1975 Jan-Feb;8(1):11–20. [PubMed] [Google Scholar]
  23. Takano K., Suzuki T., Yasuda K. Immunohistochemical localization of enterokinase in the porcine intestine. Okajimas Folia Anat Jpn. 1971 Jun;48(1):15–passim. doi: 10.2535/ofaj1936.48.1_15. [DOI] [PubMed] [Google Scholar]
  24. Tarlow M. J., Hadorn B., Arthurton M. W., Lloyd J. K. Intestinal enterokinase deficiency. A newly-recognized disorder of protein digestion. Arch Dis Child. 1970 Oct;45(243):651–655. doi: 10.1136/adc.45.243.651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Woodley J. F., Keane R. Enterokinase in normal intestinal biopsies and those from patients with untreated coeliac disease. Gut. 1972 Nov;13(11):900–902. doi: 10.1136/gut.13.11.900. [DOI] [PMC free article] [PubMed] [Google Scholar]

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