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. 1971 May;12(5):341–349. doi: 10.1136/gut.12.5.341

An ultrastructural study of the effect of neomycin on the colon in the human subject and in the conventional and the germ-free mouse

A P R Aluwihare
PMCID: PMC1411602  PMID: 5112168

Abstract

An electron microscopic study of the colon of normal mice and human subjects and those treated with neomycin is reported; there is a close resemblance between the mouse and human colons. After rapid disinfection of the colon, there is epithelial cell damage due to a toxic effect of the drug, a reduction in epithelial turnover accompanying the change in flora, and an important reduction in the cellularity of the lamina propria mainly due to a reduction in inflammatory cells.

The changes in the lamina propria probably represent changes in the antipathogenetic defences of the host.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABRAMS G. D., BAUER H., SPRINZ H. Influence of the normal flora on mucosal morphology and cellular renewal in the ileum. A comparison of germ-free and conventional mice. Lab Invest. 1963 Mar;12:355–364. [PubMed] [Google Scholar]
  2. BAUER H., HOROWITZ R. E., LEVENSON S. M., POPPER H. The response of the lymphatic tissue to the microbial flora. Studies on germfree mice. Am J Pathol. 1963 Apr;42:471–483. [PMC free article] [PubMed] [Google Scholar]
  3. BROTZU G. [Cytotoxic and cytostatic action of various antibiotics. I. Study on tissue cultures in vitro]. Minerva Chir. 1962 Dec 31;17:1254–1257. [PubMed] [Google Scholar]
  4. COHN I., Jr RECURRENCE OF CARCINOMA AFTER COLON SURGERY. Md State Med J. 1964 Jul;13:45–48. [PubMed] [Google Scholar]
  5. Corrin B., Clark A. E., Spencer H. Ultrastructural localization of acid phosphatase in the rat lung. J Anat. 1969 Jan;104(Pt 1):65–70. [PMC free article] [PubMed] [Google Scholar]
  6. DEANE H. W. SOME ELECTRON MICROSCOPIC OBSERVATIONS ON THE LAMINA PROPRIA OF THE GUT, WITH COMMENTS ON THE CLOSE ASSOCIATION OF MACROPHAGES, PLASMA CELLS, AND EOSINOPHILS. Anat Rec. 1964 Jul;149:453–473. doi: 10.1002/ar.1091490315. [DOI] [PubMed] [Google Scholar]
  7. DUBOS R. J., SCHAEDLER R. W. The effect of the intestinal flora on the growth rate of mice, and on their susceptibility to experimental infections. J Exp Med. 1960 Mar 1;111:407–417. doi: 10.1084/jem.111.3.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dobbins W. O., 3rd, Herrero B. A., Mansbach C. M. Morphologic alterations associated with neomycin induced malabsorption. Am J Med Sci. 1968 Jan;255:63–77. doi: 10.1097/00000441-196801000-00011. [DOI] [PubMed] [Google Scholar]
  9. Donnellan W. L. The structure of the colonic mucosa. The epithelium and subepithelial reticulohistiocytic complex. Gastroenterology. 1965 Nov;49(5):496–514. [PubMed] [Google Scholar]
  10. Friedmann I., Dadswell J. V., Bird E. S. Electron-microscope studies of the neuroepithelium of the inner ear in guinea-pigs treated with neomycin. J Pathol Bacteriol. 1966 Oct;92(2):415–422. doi: 10.1002/path.1700920220. [DOI] [PubMed] [Google Scholar]
  11. POTH E. J. Intestinal antisepsis; present status in surgical conditions. West J Surg Obstet Gynecol. 1952 May;60(5):205–210. [PubMed] [Google Scholar]
  12. Parker R. B. Pipette count method for obligate anaerobes. Appl Microbiol. 1965 Nov;13(6):1042–1042. doi: 10.1128/am.13.6.1042-.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pascal R. R., Kaye G. I., Lane N. Colonic pericryptal fibroblast sheath: replication, migration, and cytodifferentiation of a mesenchymal cell system in adult tissue. I. Autoradiographic studies of normal rabbit colon. Gastroenterology. 1968 May;54(5):835–851. [PubMed] [Google Scholar]
  14. Pittman F. E., Pittman J. C. An electron microscopic study of epithelium of normal human sigmoid colonic mucosa. Gut. 1966 Dec;7(6):644–661. doi: 10.1136/gut.7.6.644. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SPRINZ H., KUNDEL D. W., DAMMIN G. J., HOROWITZ R. E., SCHNEIDER H., FORMAL S. B. The response of the germfree guinea pig to oral bacterial challenge with Escherichia coli and Shigella flexneri. Am J Pathol. 1961 Dec;39:681–695. [PMC free article] [PubMed] [Google Scholar]
  16. Savage D. C., Dubos R. Alterations in the mouse cecum and its flora produced by antibacterial drugs. J Exp Med. 1968 Jul 1;128(1):97–110. doi: 10.1084/jem.128.1.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WOODWARD S. C., HERRMANN J. B., SHADOMY S., PULASKI E. J. ORAL NEOMYCIN AND THE HEALING OF COLONIC ANASTOMOSES IN THE RAT. Surg Gynecol Obstet. 1964 Oct;119:799–806. [PubMed] [Google Scholar]

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