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. 1971 Apr;12(4):311–318. doi: 10.1136/gut.12.4.311

Immunofluorescent localization of enteroglucagon cells in the gastrointestinal tract of the dog

Julia M Polak, S Bloom, I Coulling, A G E Pearse
PMCID: PMC1411617  PMID: 4930155

Abstract

Localization of the endocrine polypeptide cells responsible for `glucagon-like immunoreactivity' in the gastrointestinal tract of the dog has been achieved with an immunofluorescent technique using antibodies raised against porcine pancreatic glucagon. The cells, for which we prefer the term `enteroglucagon', could only be demonstrated by this technique in tissues fixed in carbodiimide.

The enteroglucagon cells possess cytological, cytochemical, and ultrastructural characteristics in common with those of the pancreatic α2 cell and they are equivalent in the stomach to the A cell and in the intestine to the L cell of the Wiesbaden terminology. Their distribution, predominantly in fundus and jejunum, correlates precisely with the distribution of glucagon-like immunoreactivity by radioimmunoassay and bioassay.

The storage form of enteroglucagon differs in many respects from that of pancreatic glucagon although there are some close resemblances between the two forms of specific hormone-containing granule. Elucidation of the role of enteroglucagon should be assisted by the ability to demonstrate enteroglucagon cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baxter-Grillo D. L. Enzyme histochemistry and hormones of the developing gastrointestinal tract of the chick embryo. 3. Enterochromaffin cells--their possible products, glucagon, 5-hydroxytryptamine and the relation of monoamine oxidase. Histochemie. 1970;21(2):129–135. doi: 10.1007/BF00306179. [DOI] [PubMed] [Google Scholar]
  2. Bussolati G., Pearse A. G. Immunofluorescent localization of the gastrin-secreting G cells in the pyloric antrum of the pig. Histochemie. 1970;21(1):1–4. doi: 10.1007/BF00304798. [DOI] [PubMed] [Google Scholar]
  3. COONS A. H., LEDUC E. H., CONNOLLY J. M. Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit. J Exp Med. 1955 Jul 1;102(1):49–60. doi: 10.1084/jem.102.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. De Grandi P. The routine demonstration of C cells in human and animal thyroid glands. Value of a simple silver stain. Virchows Arch B Cell Pathol. 1970;6(2):137–150. doi: 10.1007/BF02899119. [DOI] [PubMed] [Google Scholar]
  5. Forssmann W. G., Orci L., Pictet R., Renold A. E., Rouiller C. The endocrine cells in the epithelium of the gastrointestinal mucosa of the rat. An electron microscope study. J Cell Biol. 1969 Mar;40(3):692–715. doi: 10.1083/jcb.40.3.692. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  7. MAKMAN M. H., SUTHERLAND E. W., Jr USE OF LIVER ADENYL CYCLASE FOR ASSAY OF GLUCAGON IN HUMAN GASTRO-INTESTINAL TRACT AND PANCREAS. Endocrinology. 1964 Jul;75:127–134. doi: 10.1210/endo-75-1-127. [DOI] [PubMed] [Google Scholar]
  8. McGuigan J. E. Gastric mucosal intracellular localization of gastrin by immunofluorescence. Gastroenterology. 1968 Sep;55(3):315–327. [PubMed] [Google Scholar]
  9. Orci L., Pictet R., Forssmann W. G., Renold A. E., Rouiller C. Structural evidence for glucagon producing cells in the intestinal mucosa of the rat. Diabetologia. 1968 Jan;4(1):56–67. doi: 10.1007/BF01241034. [DOI] [PubMed] [Google Scholar]
  10. Pearse A. G., Bussolati G. Immunofluorescence studies on the distribution of gastrin cells in different clinical states. Gut. 1970 Aug;11(8):646–648. doi: 10.1136/gut.11.8.646. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pearse A. G. Common cytochemical and ultrastructural characteristics of cells producing polypeptide hormones (the APUD series) and their relevance to thyroid and ultimobranchial C cells and calcitonin. Proc R Soc Lond B Biol Sci. 1968 May 14;170(1018):71–80. doi: 10.1098/rspb.1968.0025. [DOI] [PubMed] [Google Scholar]
  12. Pearse A. G., Coulling I., Weavers B., Friesen S. The endocrine polypeptide cells of the human stomach, duodenum, and jejunum. Gut. 1970 Aug;11(8):649–658. doi: 10.1136/gut.11.8.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Solcia E., Sampietro R., Capella C. Differential staining of catecholamines, 5-hydroxytryptamine and related compounds in aldehyde-fixed tissues. Histochemie. 1969;17(3):273–283. doi: 10.1007/BF00309872. [DOI] [PubMed] [Google Scholar]
  14. Solcia E., Vassallo G., Sampietro R. Endocrine cells in the antro-pyloric mucosa of the stomach. Z Zellforsch Mikrosk Anat. 1967;81(4):474–486. doi: 10.1007/BF00541009. [DOI] [PubMed] [Google Scholar]
  15. Takaya K. A new fluorescent stain with o-phthalaldehyde for A cells of the pancreatic islets. J Histochem Cytochem. 1970 Mar;18(3):178–186. doi: 10.1177/18.3.178. [DOI] [PubMed] [Google Scholar]
  16. Unger R. H., Ketterer H., Eisentraut A. M. Distribution of immunoassayable glucagon in gastrointestinal tissues. Metabolism. 1966 Oct;15(10):865–867. doi: 10.1016/0026-0495(66)90156-9. [DOI] [PubMed] [Google Scholar]
  17. Unger R. H., Ohneda A., Valverde I., Eisentraut A. M., Exton J. Characterization of the responses of circulating glucagon-like immunoreactivity to intraduodenal and intravenous administration of glucose. J Clin Invest. 1968 Jan;47(1):48–65. doi: 10.1172/JCI105714. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Valverde I., Rigopoulou D., Exton J., Ohneda A., Eisentraut A., Unger R. H. Demonstration and characterization of a second fraction of glucagon-like immunoreactivity in jejunal extracts. Am J Med Sci. 1968 Jun;255:415–420. doi: 10.1097/00000441-196806000-00007. [DOI] [PubMed] [Google Scholar]
  19. Valverde I., Rigopoulou D., Marco J., Faloona G. R., Unger R. H. Characterization of glucagon-like immunoreactivity (GLI). Diabetes. 1970 Sep;19(9):614–623. doi: 10.2337/diab.19.9.614. [DOI] [PubMed] [Google Scholar]
  20. Vassallo G., Solcia E., Capella C. Light and electron microscopic identification of several types of endocrine cells in the gastrointestinal mucosa of the cat. Z Zellforsch Mikrosk Anat. 1969;98(3):333–356. doi: 10.1007/BF00346679. [DOI] [PubMed] [Google Scholar]

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