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. 1973 Sep;14(9):733–738. doi: 10.1136/gut.14.9.733

Inhibition of leucocyte migration by tumour-associated antigens of the colon and rectum

P J Guillou, G R Giles
PMCID: PMC1412774  PMID: 4201667

Abstract

The test of leucocyte migration inhibition has been used to detect lymphocyte sensitization to tumour-associated antigens of the colon and rectum. The procedure for the isolation of antigens from colorectal adenocarcinomata and normal colonic mucosa is described. The circulating lymphocytes of 22 patients suffering from colorectal adenocarcinomata of various stages have been examined and compared with the results obtained for identical studies performed on the peripheral lymphocytes of 15 control subjects. Sensitization of lymphocytes to tumour antigen was demonstrated in 15 of the 22 patients when culture was performed in homologous AB serum. Of these 15 patients only six showed inhibition of leucocyte migration in the presence of tumour extract when culture was performed in autologous serum. These observations would suggest the presence of factors in the serum of such patients which may prevent the expression of cellular immunity to tumour antigens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersen V., Bjerrum O., Bendixen G., Schiodt T., Dissing I. Effect of autologous mammary tumour extracts on human leukocyte migration in vitro. Int J Cancer. 1970 May 15;5(3):357–363. doi: 10.1002/ijc.2910050308. [DOI] [PubMed] [Google Scholar]
  2. Cochran A. J., Spilg W. G., Mackie R. M., Thomas C. E. Postoperative depression of tumour-directed cell-mediated immunity in patients with malignant disease. Br Med J. 1972 Oct 14;4(5832):67–70. [PMC free article] [PubMed] [Google Scholar]
  3. Currie G. A., Basham C. Serum mediated inhibition of the immunological reactions of the patient to his own tumour: a possible role for circulating antigen. Br J Cancer. 1972 Dec;26(6):427–438. doi: 10.1038/bjc.1972.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Currie G. A., Lejeune F., Fairley G. H. Immunization with irradiated tumour cells and specific lymphocyte cytotoxicity in malignant melanoma. Br Med J. 1971 May 8;2(5757):305–310. doi: 10.1136/bmj.2.5757.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DUKES C. E. Discussion on major surgery in carcinoma of the rectum with or without colostomy, excluding the anal canal and including the rectosigmoid: general results of surgical treatment. Proc R Soc Med. 1957 Dec;50(12):1031–1035. [PMC free article] [PubMed] [Google Scholar]
  6. Dumonde D. C. 'Lymphokines': molecular mediators of cellular immune responses in animals and man. Proc R Soc Med. 1970 Sep;63(9):899–902. [PMC free article] [PubMed] [Google Scholar]
  7. Freed D. L., Taylor G. Carcinoembryonic antigen in faeces. Br Med J. 1972 Jan 8;1(5792):85–87. doi: 10.1136/bmj.1.5792.85. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gold P., Freedman S. O. Specific carcinoembryonic antigens of the human digestive system. J Exp Med. 1965 Sep 1;122(3):467–481. doi: 10.1084/jem.122.3.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Halliday W. J. Blocking effect of serum from tumor-bearing animals on macrophage migration inhibition with tumor antigens. J Immunol. 1971 Mar;106(3):855–857. [PubMed] [Google Scholar]
  10. Hellström I., Hellström K. E., Evans C. A., Heppner G. H., Pierce G. E., Yang J. P. Serum-mediated protection of neoplastic cells from inhibition by lymphocytes immune to their tumor-specific antigens. Proc Natl Acad Sci U S A. 1969 Feb;62(2):362–368. doi: 10.1073/pnas.62.2.362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hellström I., Hellström K. E., Pierce G. E., Yang J. P. Cellular and humoral immunity to different types of human neoplasms. Nature. 1968 Dec 28;220(5174):1352–1354. doi: 10.1038/2201352a0. [DOI] [PubMed] [Google Scholar]
  12. Hellström I., Sjögren H. O., Warner G., Hellström K. E. Blocking of cell-mediated tumor immunity by sera from patients with growing neoplasms. Int J Cancer. 1971 Mar 15;7(2):226–237. doi: 10.1002/ijc.2910070206. [DOI] [PubMed] [Google Scholar]
  13. Kleinman M. S., Harwell L., Turner M. D. Studies of colonic carcinoma antigens. Gut. 1971 Jan;12(1):1–10. doi: 10.1136/gut.12.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Lejtenyi M. C., Freedman S. O., Gold P. Response of lymphocytes from patients with gastrointestinal cancer to the carcinoembryonic antigen of the human digestive system. Cancer. 1971 Jul;28(1):115–120. doi: 10.1002/1097-0142(197107)28:1<115::aid-cncr2820280121>3.0.co;2-k. [DOI] [PubMed] [Google Scholar]
  16. Martin F., Martin M. S. Radioimmunoassay of carcinoembryonic antigen in extracts of human colon and stomach. Int J Cancer. 1972 May 15;9(3):641–647. doi: 10.1002/ijc.2910090322. [DOI] [PubMed] [Google Scholar]
  17. NAIRN R. C., FOTHERGILL J. E., McENTEGART M. G., PORTEOUS I. B. Gastro-intestinal-specific antigen: an immunohistological and serological study. Br Med J. 1962 Jun 30;1(5295):1788–1790. doi: 10.1136/bmj.1.5295.1788. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nairn R. C., Nind A. P., Guli E. P., Davies D. J., Rolland J. M., McGiven A. R., Hughes E. S. Immunological reactivity in patients with carcinoma of colon. Br Med J. 1971 Dec 18;4(5789):706–709. doi: 10.1136/bmj.4.5789.706. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pick E., Turk J. L. The biological activities of soluble lymphocyte products. Clin Exp Immunol. 1972 Jan;10(1):1–23. [PMC free article] [PubMed] [Google Scholar]
  20. Wolberg W. H. Inhibition of migration of human autogenous and allogeneic leukocytes by extracts of patients' cancers. Cancer Res. 1971 Jun;31(6):798–802. [PubMed] [Google Scholar]

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