Abstract
The migration of peripheral leucocytes from patients with inflammatory bowel disease was investigated using an agarose plate technique and three antigenic materials: (1) the enterobacterial common antigen of Kunin; (2) extract from germ-free rat faeces; and (3) Kveim suspension from a sarcoid spleen K19. Inhibition of migration was obtained only with Kunin antigen. It was present in 11 out of 20 patients with ulcerative colitis and in four out of 20 patients with Crohn's disease but in only one out of 33 control subjects. These findings suggest the possibility that a state of cellular immunity to enterobacterial common antigen may exist in inflammatory bowel disease. Lack of cellular reactivity to extract from germ-free faeces and Kveim suspension is in contrast with the results obtained by others by means of the capillary tube method and requires further investigation.
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Selected References
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- Bendixen G. Cellular hypersensitivity in ulcerative colitis and Crohn's disease. Schweiz Med Wochenschr. 1971 May 22;101(20):698–701. [PubMed] [Google Scholar]
- Bendixen G. Cellular hypersensitivity to components of intestinal mucosa in ulcerative colitis and Crohn's disease. Gut. 1969 Aug;10(8):631–636. doi: 10.1136/gut.10.8.631. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brostoff J., Walker J. G. Leucocyte migration inhibition with Kveim antigen in Crohn's disease. Clin Exp Immunol. 1971 Dec;9(6):707–711. [PMC free article] [PubMed] [Google Scholar]
- Bull D. M., Ignaczak T. F. Enterobacterial common antigen-induced lymphocyte reactivity in inflammatory bowel disease. Gastroenterology. 1973 Jan;64(1):43–50. [PubMed] [Google Scholar]
- Clausen J. E. Tuberculin-induced migration inhibition of human peripheral leucocytes in agarose medium. Acta Allergol. 1971 Feb;26(1):56–80. doi: 10.1111/j.1398-9995.1971.tb01399.x. [DOI] [PubMed] [Google Scholar]
- Dykes P. W. Delayed hypersensitivity in Crohn's disease. Proc R Soc Med. 1970 Sep;63(9):906–908. [PMC free article] [PubMed] [Google Scholar]
- Hammarström S., Lagercrantz R., Perlmann P., Gustafsson B. E. Immunological studies in ulcerative colitis. II. "Colon" antigen and human blood group A- and H-like antigens in germfree rats. J Exp Med. 1965 Dec 1;122(6):1075–1085. doi: 10.1084/jem.122.6.1075. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KUNIN C. M. SEPARATION, CHARACTERIZATION, AND BIOLOGICAL SIGNIFICANCE OF A COMMON ANTIGEN IN ENTEROBACTERIACEAE. J Exp Med. 1963 Oct 1;118:565–586. doi: 10.1084/jem.118.4.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kraft S. C., Kirsner J. B. Immunological apparatus of the gut and inflammatory bowel disease. Gastroenterology. 1971 May;60(5):922–951. [PubMed] [Google Scholar]
- Lagercrantz R., Hammarström S., Perlmann P., Gustafsson B. E. Immunological studies in ulcerative colitis. IV. Origin of autoantibodies. J Exp Med. 1968 Dec 1;128(6):1339–1352. doi: 10.1084/jem.128.6.1339. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lagercrantz R., Perlmann P., Hammarström S. Immunological studies in ulcerative colitis. V. Family studies. Gastroenterology. 1971 Mar;60(3):381–389. [PubMed] [Google Scholar]
- Perlmann P., Hammarström S., Lagercrantz R., Gustafsson B. E. Antigen from colon of germfree rats and antibodies in human ulcerative colitis. Ann N Y Acad Sci. 1965 Jun 30;124(1):377–394. doi: 10.1111/j.1749-6632.1965.tb18972.x. [DOI] [PubMed] [Google Scholar]
- SUZUKI T., GORZYNSKI E. A., NETER E. SEPARATION BY ETHANOL OF COMMON AND SOMATIC ANTIGENS OF ENTEROBACTERIACEAE. J Bacteriol. 1964 Nov;88:1240–1243. doi: 10.1128/jb.88.5.1240-1243.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shorter R. G., Cardoza M., Spencer R. J., Huizenga K. A. Further studies on in vitro cytotoxicity of lymphocytes from patients with ulcerative and granulomatous colitis for allogeneic colonic epithelial cells, including the effects of colectomy. Gastroenterology. 1969 Feb;56(2):304–309. [PubMed] [Google Scholar]
- Shorter T. G., Huizenga K. A., Spencer R. J., Aas J., Guy S. K. Inflammatory bowel disease. Cytophilic antibody and the cytotoxicity of lymphocytes for colonic cells in vitro. Am J Dig Dis. 1971 Aug;16(8):673–680. doi: 10.1007/BF02239587. [DOI] [PubMed] [Google Scholar]
- Thayer W. R., Jr, Brown M., Sangree M. H., Katz J., Hersh T. Escherichia Coli O:14 and colon hemagglutinating antibodies in inflammatory bowel disease. Gastroenterology. 1969 Sep;57(3):311–318. [PubMed] [Google Scholar]
- Watson D. W., Quigley A., Bolt R. J. Effect of lymphocytes from patients with ulcerative colitis on human adult colon epithelial cells. Gastroenterology. 1966 Dec;51(6):985–993. [PubMed] [Google Scholar]
- Willoughby J. M., Mitchell D. N. In-vitro inhibition of leucocyte migration in Crohn's disease by a sarcoid spleen suspension. Br Med J. 1971 Jul 17;3(5767):155–157. doi: 10.1136/bmj.3.5767.155. [DOI] [PMC free article] [PubMed] [Google Scholar]