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. 1974 Dec;15(12):972–976. doi: 10.1136/gut.15.12.972

Studies of lactic dehydrogenase content in rectal mucosal biopsies

P S Hunt, P M Dennis, P Jablonski, S K Khoo, L McLeod, H D P Thomson, J McK Watts
PMCID: PMC1413068  PMID: 4448412

Abstract

The total lactic dehydrogenase (LD) content and LD isoenzyme ratios were studied in homogenized rectal mucosa from 31 patients with established non-specific mucosal ulcerative colitis and from 16 normal subjects. The total LD content was found to be significantly increased in patients with active ulcerative colitis when compared with patients with inactive colitis or with normal subjects. There was a similar though not a significant increase in the slow moving isoenzymes of LD in samples of rectal mucosa from patients with active colitis. The LD isoenzyme pattern was in the normal range in two of the three patients with histological premalignant changes in rectal biopsy specimens.

There was a statistically significant linear correlation between the total lactic dehydrogenase content of rectal mucosa and the carcino-embryonic antigen levels in whole serum.

Both the total content and isoenzyme ratios of LD were increased in neoplastic tissue obtained from patients with carcinoma of the colon and with tissue from benign rectal polyps.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARNETT H. THE STAINING OF LACTIC DEHYDROGENASE ISOENZYMES AFTER ELECTROPHORETIC SEPARATION ON CELLULOSE ACETATE. J Clin Pathol. 1964 Sep;17:567–570. doi: 10.1136/jcp.17.5.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Eastwood G. L., Trier J. S. Epithelial cell renewal in cultured rectal biopsies in ulcerative colitis. Gastroenterology. 1973 Mar;64(3):383–390. [PubMed] [Google Scholar]
  3. Gay R. J., McComb R. B., Bowers G. N., Jr Optimum reaction conditions for human lactate dehydrogenase isoenzymes as they affect total lactate dehydrogenase activity. Clin Chem. 1968 Aug;14(8):740–753. [PubMed] [Google Scholar]
  4. Goodfriend T. L., Sokol D. M., Kaplan N. O. Control of synthesis of lactic acid dehydrogenases. J Mol Biol. 1966 Jan;15(1):18–31. doi: 10.1016/s0022-2836(66)80206-1. [DOI] [PubMed] [Google Scholar]
  5. Khoo S. K., Hunt P. S., Mackay I. R. Studies of carcinoembryonic antigen activity of whole and extracted serum in ulcerative colitis. Gut. 1973 Jul;14(7):545–548. doi: 10.1136/gut.14.7.545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Langvad E. Lactate dehydrogenase isoenzyme patterns in the tumour-bearing colon. Int J Cancer. 1968 Jan 15;3(1):17–29. doi: 10.1002/ijc.2910030104. [DOI] [PubMed] [Google Scholar]
  7. Lewis B., Morson B. C., February A. W., Jones J. H., Misiewicz J. J. Abnormal lactic dehydrogenase isoenzyme patterns in ulcerative colitis with precancerous change. Gut. 1971 Jan;12(1):16–19. doi: 10.1136/gut.12.1.16. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Morson B. C., Pang L. S. Rectal biopsy as an aid to cancer control in ulcerative colitis. Gut. 1967 Oct;8(5):423–434. doi: 10.1136/gut.8.5.423. [DOI] [PMC free article] [PubMed] [Google Scholar]

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