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. 1974 Dec;15(12):953–959. doi: 10.1136/gut.15.12.953

Indices of granulocyte activity in inflammatory bowel disease

S P Kane, A V Hoffbrand, G Neale
PMCID: PMC1413077  PMID: 4448410

Abstract

In conditions with increased neutrophil production, the serum total vitamin B12-binding capacity (TBBC) is considered to correlate with the blood pool size of neutrophil granulocytes. The serum lysozyme, on the other hand, is a measure of neutrophil (and monocyte) turnover. The mean serum TBBC was significantly raised in patients with ulcerative colitis (range 1.23-5.51 ng/ml; mean 2.64 ng/ml) and patients with Crohn's disease (range 1.58-9.29 ng/ml; mean 2.93 ng/ml). The elevated values were shown to be due to rises in the granulocyte-secreted binding proteins, transcobalamins I and III. The TBBC was shown to rise with increasing activity of disease and to correlate roughly with the blood neutrophil granulocyte count. Patients with inflammatory bowel disease also had a significantly raised mean level of serum lysozyme (range 3.1 to 10.4 μg/ml; mean 6.8 μg/ml), but there was no correlation in individual patients between serum lysozyme and total B12-binding capacity. These results are taken to indicate an enlarged granulocyte pool and increased granulocyte turnover in inflammatory bowel disease.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSON B. B. INVESTIGATIONS INTO THE EUGLENA METHOD FOR THE ASSAY OF THE VITAMIN B12 IN SERUM. J Clin Pathol. 1964 Jan;17:14–26. doi: 10.1136/jcp.17.1.14. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ANTHONISEN P., RIIS P. The cytology of colonic secretion in proctosigmoidal disease. Acta Med Scand. 1962 Sep;172:375–381. doi: 10.1111/j.0954-6820.1962.tb07168.x. [DOI] [PubMed] [Google Scholar]
  3. Bloomfield F. J., Scott J. M. Identification of a new vitamin B 12 binder (transcobalamin 3) in normal human serum. Br J Haematol. 1972 Jan;22(1):33–42. doi: 10.1111/j.1365-2141.1972.tb08784.x. [DOI] [PubMed] [Google Scholar]
  4. Bloomfield F. J., Scott J. M., Somerville J. J., Weir D. G. Levels in normal, pathological, and foetal sera of the three transcobalamins. Ir J Med Sci. 1973 Mar;142(2):51–57. doi: 10.1007/BF02949990. [DOI] [PubMed] [Google Scholar]
  5. Britt R. P., Rose D. P. Pernicious anemia with a normal serum vitamin B-12 level in a case of chronic granulocytic leukemia. Arch Intern Med. 1966 Jan;117(1):32–33. [PubMed] [Google Scholar]
  6. Carmel R., Coltman C. A., Jr Monocytic vitamin B12-binding protein. Blood. 1971 Mar;37(3):360–366. [PubMed] [Google Scholar]
  7. Carmel R., Herbert V. Vitamin B 12 -binding protein of leukocytes as a possible major source of the third vitamin B 12 -binding protein of serum. Blood. 1972 Oct;40(4):542–549. [PubMed] [Google Scholar]
  8. Carmel R. Vitamin B 12 -binding protein abnormality in subjects without myeloproliferative disease. II. The presence of a third vitamin B 12 -binding protein in serum. Br J Haematol. 1972 Jan;22(1):53–62. doi: 10.1111/j.1365-2141.1972.tb08786.x. [DOI] [PubMed] [Google Scholar]
  9. Carmel R. Vitamin B 12-binding protein abnormality in subjects without myeloproliferative disease. I. Elevated serum vitamin B 12-binding capacity levels in patients with leucocytosis. Br J Haematol. 1972 Jan;22(1):43–51. doi: 10.1111/j.1365-2141.1972.tb08785.x. [DOI] [PubMed] [Google Scholar]
  10. Catovsky D., Galton D. A., Griffin C., Hoffbrand A. V., Szur L. Serum lysozyme and vitamin B 12 binding capacity in myeloproliferative disorders. Br J Haematol. 1971 Dec;21(6):661–672. doi: 10.1111/j.1365-2141.1971.tb02728.x. [DOI] [PubMed] [Google Scholar]
  11. Chikkappa G., Corcino J., Greenerg M. L., Herert V. Correation between vario blood white cell pools and the serum B12-binding capaities. Blood. 1971 Feb;37(2):142–151. [PubMed] [Google Scholar]
  12. Corcino J. J., Zalusky R., Greenberg M., Herbert V. Coexistence of pernicious anaemia and chronic myeloid leukaemia: an experiment of nature involving vitamin B12 metabolism. Br J Haematol. 1971 May;20(5):511–520. doi: 10.1111/j.1365-2141.1971.tb07066.x. [DOI] [PubMed] [Google Scholar]
  13. Corcino J., Krauss S., Waxman S., Herbert V. Release of vitamin B12--binding protein by human leukocytes in vitro. J Clin Invest. 1970 Dec;49(12):2250–2255. doi: 10.1172/JCI106444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Fink M. E., Finch S. C. Serum muramidase and granulocyte turnover. Proc Soc Exp Biol Med. 1968 Feb;127(2):365–367. doi: 10.3181/00379727-127-32692. [DOI] [PubMed] [Google Scholar]
  15. GOTTLIEBLAU K. S., WASSERMAN L. R., HERBERT V. RAPID CHARCOAL ASSAY FOR INTRINSIC FACTOR (IF), GASTRIC JUICE UNSATURATED B12 BINDING CAPACITY, ANTIBODY TO IF, AND SERUM UNSATURATED B12 BINDING CAPACITY. Blood. 1965 Jun;25:875–884. [PubMed] [Google Scholar]
  16. Hansen N. E. The relationship between the turnover rate of neutrophilic granulocytes and plasma lysozyme levels. Br J Haematol. 1973 Dec;25(6):771–782. doi: 10.1111/j.1365-2141.1973.tb01789.x. [DOI] [PubMed] [Google Scholar]
  17. Herbert V. Diagnostic and prognostic values of measurement of serum vitamin B12-binding proteins. Blood. 1968 Aug;32(2):305–312. [PubMed] [Google Scholar]
  18. Levi J. A., Macqueen A., Vincent P. C. Assessment of the value of lysozyme assay in neutropenia. Br J Haematol. 1973 Dec;25(6):757–770. doi: 10.1111/j.1365-2141.1973.tb01788.x. [DOI] [PubMed] [Google Scholar]
  19. Osserman E. F., Lawlor D. P. Serum and urinary lysozyme (muramidase) in monocytic and monomyelocytic leukemia. J Exp Med. 1966 Nov 1;124(5):921–952. doi: 10.1084/jem.124.5.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. PARRY R. M., Jr, CHANDAN R. C., SHAHANI K. M. A RAPID AND SENSITIVE ASSAY OF MURAMIDASE. Proc Soc Exp Biol Med. 1965 Jun;119:384–386. doi: 10.3181/00379727-119-30188. [DOI] [PubMed] [Google Scholar]
  21. Pascual R. S., Gee J. B., Finch S. C. Usefulness of serum lysozyme measurement in diagnosis and evaluation of sarcoidosis. N Engl J Med. 1973 Nov 15;289(20):1074–1076. doi: 10.1056/NEJM197311152892007. [DOI] [PubMed] [Google Scholar]
  22. Perillie P. E., Kaplan S. S., Finch S. C. Significance of changes in serum muramidase activity in megaloblastic anemia. N Engl J Med. 1967 Jul 6;277(1):10–12. doi: 10.1056/NEJM196707062770103. [DOI] [PubMed] [Google Scholar]
  23. Riis P., Anthonisen P. The presence of inflammatory leucocytes in the rectal mucus of patients with regional ileitis. Scand J Gastroenterol. 1971;6(8):719–721. doi: 10.3109/00365527109179942. [DOI] [PubMed] [Google Scholar]
  24. Silverstein E., Herbert V. Rapid determination of vitamin B12-binding alpha- and beta-globulin in serum. Blood. 1968 Apr;31(4):518–521. [PubMed] [Google Scholar]
  25. Zittoun R., Zittoun J. Les transcobalamines. Nouv Presse Med. 1973 Apr 7;2(14):919–924. [PubMed] [Google Scholar]

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