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. 1994 May;82(1):154–163.

A novel internal antigen which distinguishes germinal centre cells from other B-cell types.

K Pulford 1, K J Micklem 1, M Jones 1, F Pezzella 1, K M Mayne 1, H Morrison 1, J Thomas 1, B Falini 1, A Norton 1, D Y Mason 1
PMCID: PMC1414835  PMID: 8045591

Abstract

A new monoclonal antibody, 4KB51, is described which labels the majority of B cells in blood and in mantle and marginal zones but not germinal centre lymphocytes or plasma cells. Antibody 4KB51 also stains monocytes, neutrophils and the majority of T cells. It recognizes an intracellular antigen of 160,000 MW (unreduced) and 68,000 MW (reduced). Antibody 4KB51 labels the tumour cells in all cases of hairy cell leukaemia and in four of the 16 cases of centrocytic B-cell lymphoma studied. No labelling of the other lymphomas (114 cases) or lymphoid leukaemias (13 cases) tested was seen. Antibody 4KB51 may be of value in defining B-cell subsets and in the differential diagnosis of hairy cell leukaemia and centrocytic lymphomas. The pattern of reactivity of 4KB51 suggests that its target antigen may play a functional role, possibly involved in lymphocyte homing.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson K. C., Bates M. P., Slaughenhoupt B. L., Pinkus G. S., Schlossman S. F., Nadler L. M. Expression of human B cell-associated antigens on leukemias and lymphomas: a model of human B cell differentiation. Blood. 1984 Jun;63(6):1424–1433. [PubMed] [Google Scholar]
  2. Anderson K. C., Boyd A. W., Fisher D. C., Leslie D., Schlossman S. F., Nadler L. M. Hairy cell leukemia: a tumor of pre-plasma cells. Blood. 1985 Mar;65(3):620–629. [PubMed] [Google Scholar]
  3. Aruffo A., Stamenkovic I., Melnick M., Underhill C. B., Seed B. CD44 is the principal cell surface receptor for hyaluronate. Cell. 1990 Jun 29;61(7):1303–1313. doi: 10.1016/0092-8674(90)90694-a. [DOI] [PubMed] [Google Scholar]
  4. Banks P. M., Chan J., Cleary M. L., Delsol G., De Wolf-Peeters C., Gatter K., Grogan T. M., Harris N. L., Isaacson P. G., Jaffe E. S. Mantle cell lymphoma. A proposal for unification of morphologic, immunologic, and molecular data. Am J Surg Pathol. 1992 Jul;16(7):637–640. doi: 10.1097/00000478-199207000-00001. [DOI] [PubMed] [Google Scholar]
  5. Clark E. A., Lane P. J. Regulation of human B-cell activation and adhesion. Annu Rev Immunol. 1991;9:97–127. doi: 10.1146/annurev.iy.09.040191.000525. [DOI] [PubMed] [Google Scholar]
  6. Cordell J. L., Falini B., Erber W. N., Ghosh A. K., Abdulaziz Z., MacDonald S., Pulford K. A., Stein H., Mason D. Y. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem. 1984 Feb;32(2):219–229. doi: 10.1177/32.2.6198355. [DOI] [PubMed] [Google Scholar]
  7. Dilosa R. M., Maeda K., Masuda A., Szakal A. K., Tew J. G. Germinal center B cells and antibody production in the bone marrow. J Immunol. 1991 Jun 15;146(12):4071–4077. [PubMed] [Google Scholar]
  8. Erber W. N., Pinching A. J., Mason D. Y. Immunocytochemical detection of T and B cell populations in routine blood smears. Lancet. 1984 May 12;1(8385):1042–1046. doi: 10.1016/s0140-6736(84)91451-x. [DOI] [PubMed] [Google Scholar]
  9. Fend F., Nachbaur D., Oberwasserlechner F., Kreczy A., Huber H., Müller-Hermelink H. K. Phenotype and topography of human thymic B cells. An immunohistologic study. Virchows Arch B Cell Pathol Incl Mol Pathol. 1991;60(6):381–388. doi: 10.1007/BF02899570. [DOI] [PubMed] [Google Scholar]
  10. Fujii Y., Okumura M., Inada K., Nakahara K., Matsuda H. CD45 isoform expression during T cell development in the thymus. Eur J Immunol. 1992 Jul;22(7):1843–1850. doi: 10.1002/eji.1830220725. [DOI] [PubMed] [Google Scholar]
  11. Funaro A., Spagnoli G. C., Ausiello C. M., Alessio M., Roggero S., Delia D., Zaccolo M., Malavasi F. Involvement of the multilineage CD38 molecule in a unique pathway of cell activation and proliferation. J Immunol. 1990 Oct 15;145(8):2390–2396. [PubMed] [Google Scholar]
  12. Gerdes J., Schwab U., Lemke H., Stein H. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 1983 Jan 15;31(1):13–20. doi: 10.1002/ijc.2910310104. [DOI] [PubMed] [Google Scholar]
  13. Goding J. W., Burns G. F. Monoclonal antibody OKT-9 recognizes the receptor for transferrin on human acute lymphocytic leukemia cells. J Immunol. 1981 Sep;127(3):1256–1258. [PubMed] [Google Scholar]
  14. Hollema H., Poppema S. Immunophenotypes of malignant lymphoma centroblastic-centrocytic and malignant lymphoma centrocytic: an immunohistologic study indicating a derivation from different stages of B cell differentiation. Hum Pathol. 1988 Sep;19(9):1053–1059. doi: 10.1016/s0046-8177(88)80086-8. [DOI] [PubMed] [Google Scholar]
  15. Kansas G. S., Wood G. S., Engleman E. G. Maturational and functional diversity of human B lymphocytes delineated with anti-Leu-8. J Immunol. 1985 May;134(5):3003–3006. [PubMed] [Google Scholar]
  16. Kansas G. S., Wood G. S., Fishwild D. M., Engleman E. G. Functional characterization of human T lymphocyte subsets distinguished by monoclonal anti-leu-8. J Immunol. 1985 May;134(5):2995–3002. [PubMed] [Google Scholar]
  17. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  18. Mangeney M., Richard Y., Coulaud D., Tursz T., Wiels J. CD77: an antigen of germinal center B cells entering apoptosis. Eur J Immunol. 1991 May;21(5):1131–1140. doi: 10.1002/eji.1830210507. [DOI] [PubMed] [Google Scholar]
  19. Mason D. Y., Abdulaziz Z., Falini B., Stein H. Single and double immunoenzymatic techniques for labeling tissue sections with monoclonal antibodies. Ann N Y Acad Sci. 1983;420:127–133. doi: 10.1111/j.1749-6632.1983.tb22196.x. [DOI] [PubMed] [Google Scholar]
  20. Mason D. Y., Cordell J. L., Tse A. G., van Dongen J. J., van Noesel C. J., Micklem K., Pulford K. A., Valensi F., Comans-Bitter W. M., Borst J. The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol. 1991 Dec 1;147(11):2474–2482. [PubMed] [Google Scholar]
  21. Mayne K. M., Pulford K., Jones M., Micklem K., Nagel G., van der Schoot C. E., Mason D. Y. Antibody By114 is selective for the 90 kD PI-linked component of the CD66 antigen: a new reagent for the study of paroxysmal nocturnal haemoglobinuria. Br J Haematol. 1993 Jan;83(1):30–38. doi: 10.1111/j.1365-2141.1993.tb04627.x. [DOI] [PubMed] [Google Scholar]
  22. Micklem K. J., Stross W. P., Willis A. C., Cordell J. L., Jones M., Mason D. Y. Different isoforms of human FcRII distinguished by CDw32 antibodies. J Immunol. 1990 Mar 15;144(6):2295–2303. [PubMed] [Google Scholar]
  23. Naiem M., Gerdes J., Abdulaziz Z., Sunderland C. A., Allington M. J., Stein H., Mason D. Y. The value of immunohistological screening in the production of monoclonal antibodies. J Immunol Methods. 1982;50(2):145–160. doi: 10.1016/0022-1759(82)90221-6. [DOI] [PubMed] [Google Scholar]
  24. Poletti A., Manconi R., De Paoli P. Double labeling immunohistologic and flow cytometric analysis of human B cells with particular reference to Leu-8 expression. Hum Pathol. 1988 Sep;19(9):1001–1007. doi: 10.1016/s0046-8177(88)80077-7. [DOI] [PubMed] [Google Scholar]
  25. Pulford K. A., Gatter K. C., Mason D. Y. The characterization of two monoclonal anti-keratin antibodies and their use in the study of epithelial disorders. Histopathology. 1985 Aug;9(8):825–840. doi: 10.1111/j.1365-2559.1985.tb02869.x. [DOI] [PubMed] [Google Scholar]
  26. Pulido R., Cebrián M., Acevedo A., de Landázuri M. O., Sánchez-Madrid F. Comparative biochemical and tissue distribution study of four distinct CD45 antigen specificities. J Immunol. 1988 Jun 1;140(11):3851–3857. [PubMed] [Google Scholar]
  27. Shipp M. A., Stefano G. B., Switzer S. N., Griffin J. D., Reinherz E. L. CD10 (CALLA)/neutral endopeptidase 24.11 modulates inflammatory peptide-induced changes in neutrophil morphology, migration, and adhesion proteins and is itself regulated by neutrophil activation. Blood. 1991 Oct 1;78(7):1834–1841. [PubMed] [Google Scholar]
  28. Slaper-Cortenbach I. C., Admiraal L. G., Kerr J. M., van Leeuwen E. F., von dem Borne A. E., Tetteroo P. A. Flow-cytometric detection of terminal deoxynucleotidyl transferase and other intracellular antigens in combination with membrane antigens in acute lymphatic leukemias. Blood. 1988 Nov;72(5):1639–1644. [PubMed] [Google Scholar]
  29. Spencer J., Choy M., Hussell T., Papadaki L., Kington J. P., Isaacson P. G. Properties of human thymic B cells. Immunology. 1992 Apr;75(4):596–600. [PMC free article] [PubMed] [Google Scholar]
  30. Swerdlow S. H., Habeshaw J. A., Murray L. J., Dhaliwal H. S., Lister T. A., Stansfeld A. G. Centrocytic lymphoma: a distinct clinicopathologic and immunologic entity. A multiparameter study of 18 cases at diagnosis and relapse. Am J Pathol. 1983 Nov;113(2):181–197. [PMC free article] [PubMed] [Google Scholar]
  31. Tedder T. F., Matsuyama T., Rothstein D., Schlossman S. F., Morimoto C. Human antigen-specific memory T cells express the homing receptor (LAM-1) necessary for lymphocyte recirculation. Eur J Immunol. 1990 Jun;20(6):1351–1355. doi: 10.1002/eji.1830200622. [DOI] [PubMed] [Google Scholar]
  32. Tolksdorf G., Stein H., Lennert K. Morphological and immunological definition of a malignant lymphoma derived from germinal-centre cells with cleaved nuclei (centrocytes). Br J Cancer. 1980 Feb;41(2):168–182. doi: 10.1038/bjc.1980.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Vaux D. L., Cory S., Adams J. M. Bcl-2 gene promotes haemopoietic cell survival and cooperates with c-myc to immortalize pre-B cells. Nature. 1988 Sep 29;335(6189):440–442. doi: 10.1038/335440a0. [DOI] [PubMed] [Google Scholar]
  34. Weisenburger D. D. Non-Hodgkin's lymphomas of primary follicle/mantle zone origin. Leukemia. 1991;5 (Suppl 1):26–29. [PubMed] [Google Scholar]

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