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. 1995 Jan;84(1):142–147.

Alveolar macrophages from humans and rodents selectively inhibit T-cell proliferation but permit T-cell activation and cytokine secretion.

J W Upham 1, D H Strickland 1, N Bilyk 1, B W Robinson 1, P G Holt 1
PMCID: PMC1415174  PMID: 7534261

Abstract

Alveolar macrophages (AM) are thought to play a key role in the regulation of immune responses within the lung. While it is well established that AM inhibit T-cell proliferation in vitro, it is unclear whether other aspects of the T-cell activation process are also inhibited. The present study demonstrates that AM from rat, mouse and human differ markedly in the potency with which they inhibit mitogen-induced T-cell proliferation, although in humans the degree of inhibition approaches that observed in the animal systems, if antigen (as opposed to mitogen) is employed as the T-cell activating agent. Rodent and human AM also differ in the mechanisms employed to achieve this inhibition; rodent AM appear to utilize reactive nitrogen intermediates, while this does not appear to be the case for human AM. Despite these differences, T cells stimulated in the presence of AM display a similar phenotype in all species examined, i.e. CD3 down-modulation, up-regulation of interleukin-2 receptor (IL-2R) expression and IL-2 production, but inability to respond to IL-2. Thus, AM appear to allow T-cell activation and expression of T-cell effector function, while selectively inhibiting T-cell proliferation.

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Selected References

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  1. Albina J. E., Abate J. A., Henry W. L., Jr Nitric oxide production is required for murine resident peritoneal macrophages to suppress mitogen-stimulated T cell proliferation. Role of IFN-gamma in the induction of the nitric oxide-synthesizing pathway. J Immunol. 1991 Jul 1;147(1):144–148. [PubMed] [Google Scholar]
  2. Bilyk N., Mackenzie J. S., Papadimitriou J. M., Holt P. G. Functional studies on macrophage populations in the airways and the lung wall of SPF mice in the steady-state and during respiratory virus infection. Immunology. 1988 Nov;65(3):417–425. [PMC free article] [PubMed] [Google Scholar]
  3. Cameron M. L., Granger D. L., Weinberg J. B., Kozumbo W. J., Koren H. S. Human alveolar and peritoneal macrophages mediate fungistasis independently of L-arginine oxidation to nitrite or nitrate. Am Rev Respir Dis. 1990 Dec;142(6 Pt 1):1313–1319. doi: 10.1164/ajrccm/142.6_Pt_1.1313. [DOI] [PubMed] [Google Scholar]
  4. Daynes R. A., Araneo B. A., Dowell T. A., Huang K., Dudley D. Regulation of murine lymphokine production in vivo. III. The lymphoid tissue microenvironment exerts regulatory influences over T helper cell function. J Exp Med. 1990 Apr 1;171(4):979–996. doi: 10.1084/jem.171.4.979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ferro T. J., Kern J. A., Elias J. A., Kamoun M., Daniele R. P., Rossman M. D. Alveolar macrophages, blood monocytes, and density-fractionated alveolar macrophages differ in their ability to promote lymphocyte proliferation to mitogen and antigen. Am Rev Respir Dis. 1987 Mar;135(3):682–687. doi: 10.1164/arrd.1987.135.3.682. [DOI] [PubMed] [Google Scholar]
  6. Fu Y., Blankenhorn E. P. Nitric oxide-induced anti-mitogenic effects in high and low responder rat strains. J Immunol. 1992 Apr 1;148(7):2217–2222. [PubMed] [Google Scholar]
  7. Holt P. G. Alveolar macrophages. IV. Interspecies differences in activity in proliferating lymphocyte cultures. Cell Immunol. 1980 Mar 1;50(1):210–215. doi: 10.1016/0008-8749(80)90020-9. [DOI] [PubMed] [Google Scholar]
  8. Holt P. G. Down-regulation of immune responses in the lower respiratory tract: the role of alveolar macrophages. Clin Exp Immunol. 1986 Feb;63(2):261–270. [PMC free article] [PubMed] [Google Scholar]
  9. Holt P. G. Regulation of antigen-presenting cell function(s) in lung and airway tissues. Eur Respir J. 1993 Jan;6(1):120–129. [PubMed] [Google Scholar]
  10. Holt P. G., Schon-Hegrad M. A., Oliver J. MHC class II antigen-bearing dendritic cells in pulmonary tissues of the rat. Regulation of antigen presentation activity by endogenous macrophage populations. J Exp Med. 1988 Feb 1;167(2):262–274. doi: 10.1084/jem.167.2.262. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Huot A. E., Moore A. L., Roberts J. D., Hacker M. P. Nitric oxide modulates lymphocyte proliferation but not secretion of IL-2. Immunol Invest. 1993 Jun;22(4):319–327. doi: 10.3109/08820139309063411. [DOI] [PubMed] [Google Scholar]
  12. Kawabe T., Isobe K. I., Hasegawa Y., Nakashima I., Shimokata K. Immunosuppressive activity induced by nitric oxide in culture supernatant of activated rat alveolar macrophages. Immunology. 1992 May;76(1):72–78. [PMC free article] [PubMed] [Google Scholar]
  13. Kobzik L., Bredt D. S., Lowenstein C. J., Drazen J., Gaston B., Sugarbaker D., Stamler J. S. Nitric oxide synthase in human and rat lung: immunocytochemical and histochemical localization. Am J Respir Cell Mol Biol. 1993 Oct;9(4):371–377. doi: 10.1165/ajrcmb/9.4.371. [DOI] [PubMed] [Google Scholar]
  14. Marletta M. A., Yoon P. S., Iyengar R., Leaf C. D., Wishnok J. S. Macrophage oxidation of L-arginine to nitrite and nitrate: nitric oxide is an intermediate. Biochemistry. 1988 Nov 29;27(24):8706–8711. doi: 10.1021/bi00424a003. [DOI] [PubMed] [Google Scholar]
  15. Mason D. W., Arthur R. P., Dallman M. J., Green J. R., Spickett G. P., Thomas M. L. Functions of rat T-lymphocyte subsets isolated by means of monoclonal antibodies. Immunol Rev. 1983;74:57–82. doi: 10.1111/j.1600-065x.1983.tb01084.x. [DOI] [PubMed] [Google Scholar]
  16. McCombs C. C., Michalski J. P., Westerfield B. T., Light R. W. Human alveolar macrophages suppress the proliferative response of peripheral blood lymphocytes. Chest. 1982 Sep;82(3):266–271. doi: 10.1378/chest.82.3.266. [DOI] [PubMed] [Google Scholar]
  17. Merryman P. F., Clancy R. M., He X. Y., Abramson S. B. Modulation of human T cell responses by nitric oxide and its derivative, S-nitrosoglutathione. Arthritis Rheum. 1993 Oct;36(10):1414–1422. doi: 10.1002/art.1780361014. [DOI] [PubMed] [Google Scholar]
  18. Moore S. A., Strieter R. M., Rolfe M. W., Standiford T. J., Burdick M. D., Kunkel S. L. Expression and regulation of human alveolar macrophage-derived interleukin-1 receptor antagonist. Am J Respir Cell Mol Biol. 1992 Jun;6(6):569–575. doi: 10.1165/ajrcmb/6.6.569. [DOI] [PubMed] [Google Scholar]
  19. Nicolls M. R., Aversa G. G., Pearce N. W., Spinelli A., Berger M. F., Gurley K. E., Hall B. M. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody. Transplantation. 1993 Mar;55(3):459–468. doi: 10.1097/00007890-199303000-00001. [DOI] [PubMed] [Google Scholar]
  20. Paine R., 3rd, Mody C. H., Chavis A., Spahr M. A., Turka L. A., Toews G. B. Alveolar epithelial cells block lymphocyte proliferation in vitro without inhibiting activation. Am J Respir Cell Mol Biol. 1991 Sep;5(3):221–229. doi: 10.1165/ajrcmb/5.3.221. [DOI] [PubMed] [Google Scholar]
  21. Paliogianni F., Ahuja S. S., Balow J. P., Balow J. E., Boumpas D. T. Novel mechanism for inhibition of human T cells by glucocorticoids. Glucocorticoids inhibit signal transduction through IL-2 receptor. J Immunol. 1993 Oct 15;151(8):4081–4089. [PubMed] [Google Scholar]
  22. Paterson D. J., Jefferies W. A., Green J. R., Brandon M. R., Corthesy P., Puklavec M., Williams A. F. Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts. Mol Immunol. 1987 Dec;24(12):1281–1290. doi: 10.1016/0161-5890(87)90122-2. [DOI] [PubMed] [Google Scholar]
  23. Rich E. A., Cooper C., Toossi Z., Leonard M. L., Stucky R. M., Wiblin R. T., Ellner J. J. Requirement for cell-to-cell contact for the immunosuppressive activity of human alveolar macrophages. Am J Respir Cell Mol Biol. 1991 Mar;4(3):287–294. doi: 10.1165/ajrcmb/4.3.287. [DOI] [PubMed] [Google Scholar]
  24. Robinson B. W., James A., Rose A. H., Sterrett G. F., Musk A. W. Bronchoalveolar lavage sampling of airway and alveolar cells. Br J Dis Chest. 1988 Jan;82(1):45–55. doi: 10.1016/0007-0971(88)90007-1. [DOI] [PubMed] [Google Scholar]
  25. Schneemann M., Schoedon G., Hofer S., Blau N., Guerrero L., Schaffner A. Nitric oxide synthase is not a constituent of the antimicrobial armature of human mononuclear phagocytes. J Infect Dis. 1993 Jun;167(6):1358–1363. doi: 10.1093/infdis/167.6.1358. [DOI] [PubMed] [Google Scholar]
  26. Strickland D. H., Thepen T., Kees U. R., Kraal G., Holt P. G. Regulation of T-cell function in lung tissue by pulmonary alveolar macrophages. Immunology. 1993 Oct;80(2):266–272. [PMC free article] [PubMed] [Google Scholar]
  27. Suzuki G., Kawase Y., Koyasu S., Yahara I., Kobayashi Y., Schwartz R. H. Antigen-induced suppression of the proliferative response of T cell clones. J Immunol. 1988 Mar 1;140(5):1359–1365. [PubMed] [Google Scholar]
  28. Thepen T., McMenamin C., Girn B., Kraal G., Holt P. G. Regulation of IgE production in pre-sensitized animals: in vivo elimination of alveolar macrophages preferentially increases IgE responses to inhaled allergen. Clin Exp Allergy. 1992 Dec;22(12):1107–1114. doi: 10.1111/j.1365-2222.1992.tb00137.x. [DOI] [PubMed] [Google Scholar]
  29. Thepen T., Van Rooijen N., Kraal G. Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice. J Exp Med. 1989 Aug 1;170(2):499–509. doi: 10.1084/jem.170.2.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Tomonari K. A rat antibody against a structure functionally related to the mouse T-cell receptor/T3 complex. Immunogenetics. 1988;28(6):455–458. doi: 10.1007/BF00355379. [DOI] [PubMed] [Google Scholar]
  31. al-Ramadi B. K., Meissler J. J., Jr, Huang D., Eisenstein T. K. Immunosuppression induced by nitric oxide and its inhibition by interleukin-4. Eur J Immunol. 1992 Sep;22(9):2249–2254. doi: 10.1002/eji.1830220911. [DOI] [PubMed] [Google Scholar]

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