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. 1981 Jan;22(1):19–24. doi: 10.1136/gut.22.1.19

Description and basic cell kinetics of the murine pericryptal fibroblast sheath.

J V Neal, C S Potten
PMCID: PMC1419298  PMID: 7461472

Abstract

The size of the pericryptal fibroblast sheath (PCFS) population was determined by scoring serial sections. There are 38 and 124 PCFS cells per murine small intestinal and colonic crypt, respectively. The cells of the PCFS are slightly weighted towards the lower two-thirds of the crypt in their distribution. The ratio of epithelial cells to PCFS cells is approximately 6.5:1. The in vivo cell kinetics were analysed under control and stressed (fasted-refed) conditions. The control labelling index increases from 8.9% in the small intestine and 7.0% in the colon to peaks 49% and 113% respectively above these values 24 hours after 3HTdR administration. Labelling is observed at all crypt levels equally, and no evidence of vertical migration of labelled PCFS cells was found. Colonic epithelial and PCFS cells show a similar pattern of response to feeding after a fast of 72 hours with respect to time, but a different distribution of response in terms of crypt position.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldewachi H. S., Wright N. A., Appleton D. R., Watson A. J. The effect of starvation and refeeding on cell population kinetics in the rat small bowel mucosa. J Anat. 1975 Feb;119(Pt 1):105–121. [PMC free article] [PubMed] [Google Scholar]
  2. BENCOSME S. A. A trichrome staining method for routine use. Am J Clin Pathol. 1954 Nov;24(11):1324–1328. doi: 10.1093/ajcp/24.11_ts.1324. [DOI] [PubMed] [Google Scholar]
  3. Cairnie A. B., Lamerton L. F., Steel G. G. Cell proliferation studies in the intestinal epithelium of the rat. I. Determination of the kinetic parameters. Exp Cell Res. 1965 Sep;39(2):528–538. doi: 10.1016/0014-4827(65)90055-8. [DOI] [PubMed] [Google Scholar]
  4. Cairnie A. B. Renewal of goblet and Paneth cells in the small intestine. Cell Tissue Kinet. 1970 Jan;3(1):35–45. doi: 10.1111/j.1365-2184.1970.tb00250.x. [DOI] [PubMed] [Google Scholar]
  5. Cheng H., Leblond C. P. Origin, differentiation and renewal of the four main epithelial cell types in the mouse small intestine. III. Entero-endocrine cells. Am J Anat. 1974 Dec;141(4):503–519. doi: 10.1002/aja.1001410405. [DOI] [PubMed] [Google Scholar]
  6. Clarke R. M. The time-course of changes in mucosal architecture and epithelial cell production and cell shedding in the small intestine of the rat fed after fasting. J Anat. 1975 Nov;120(Pt 2):321–327. [PMC free article] [PubMed] [Google Scholar]
  7. DEANE H. W. SOME ELECTRON MICROSCOPIC OBSERVATIONS ON THE LAMINA PROPRIA OF THE GUT, WITH COMMENTS ON THE CLOSE ASSOCIATION OF MACROPHAGES, PLASMA CELLS, AND EOSINOPHILS. Anat Rec. 1964 Jul;149:453–473. doi: 10.1002/ar.1091490315. [DOI] [PubMed] [Google Scholar]
  8. Donnellan W. L. The structure of the colonic mucosa. The epithelium and subepithelial reticulohistiocytic complex. Gastroenterology. 1965 Nov;49(5):496–514. [PubMed] [Google Scholar]
  9. Hagemann R. F., Stragand J. J. Fasting and refeeding: cell kinetic response of jejunum, ileum and colon. Cell Tissue Kinet. 1977 Jan;10(1):3–14. doi: 10.1111/j.1365-2184.1977.tb00278.x. [DOI] [PubMed] [Google Scholar]
  10. Kaye G. I., Lane N., Pascal R. R. Colonic pericryptal fibroblast sheath: replication, migration, and cytodifferentiation of a mesenchymal cell system in adult tissue. II. Fine structural aspects of normal rabbit and human colon. Gastroenterology. 1968 May;54(5):852–865. [PubMed] [Google Scholar]
  11. Kaye G. I., Maenza R. M., Lane N. Cell replication in rabbit gall bladder. An autoradiographic study of epithelial and associated fibroblast renewal in vivo and in vitro. Gastroenterology. 1966 Nov;51(5):670–680. [PubMed] [Google Scholar]
  12. Kaye G. I., Pascal R. R., Lane N. The colonic pericryptal fibroblast sheath: replication, migration, and cytodifferentiation of a mesenchymal cell system in adult tissue. 3. Replication and differentiation in human hyperplastic and adenomatous polyps. Gastroenterology. 1971 Apr;60(4):515–536. [PubMed] [Google Scholar]
  13. Marsh M. N., Trier J. S. Morphology and cell proliferation of subepithelial fibroblasts in adult mouse jejunum. I. Structural features. Gastroenterology. 1974 Oct;67(4):622–635. [PubMed] [Google Scholar]
  14. Marsh M. N., Trier J. S. Morphology and cell proliferation of subepithelial fibroblasts in adult mouse jejunum. II. Radioautographic studies. Gastroenterology. 1974 Oct;67(4):636–645. [PubMed] [Google Scholar]
  15. Maskens A. P., Rahier J. R., Meersseman F. P., Dujardin-Loits R. M., Haot J. G. Cell proliferation of pericryptal fibroblasts in the rat colon mucosa. Gut. 1979 Sep;20(9):775–779. doi: 10.1136/gut.20.9.775. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PELC S. R. LABELLING OF DNA AND CELL DIVISION IN SO CALLED NON-DIVIDING TISSUES. J Cell Biol. 1964 Jul;22:21–28. doi: 10.1083/jcb.22.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Parker F. G., Barnes E. N., Kaye G. I. The pericryptal fibroblast sheath. IV. Replication, migration, and differentiation of the subepithelial fibroblasts of the crypt and villus of the rabbit jejunum. Gastroenterology. 1974 Oct;67(4):607–621. [PubMed] [Google Scholar]
  18. Pascal R. R., Kaye G. I., Lane N. Colonic pericryptal fibroblast sheath: replication, migration, and cytodifferentiation of a mesenchymal cell system in adult tissue. I. Autoradiographic studies of normal rabbit colon. Gastroenterology. 1968 May;54(5):835–851. [PubMed] [Google Scholar]
  19. Potten C. S. Further observations on the late labelling associated with stimulus-responsive cells in skin. Cell Tissue Kinet. 1973 Nov;6(6):553–566. doi: 10.1111/j.1365-2184.1973.tb01646.x. [DOI] [PubMed] [Google Scholar]
  20. QUASTLER H., SHERMAN F. G. Cell population kinetics in the intestinal epithelium of the mouse. Exp Cell Res. 1959 Jun;17(3):420–438. doi: 10.1016/0014-4827(59)90063-1. [DOI] [PubMed] [Google Scholar]
  21. Rijke R. P., Plaisier H. M., Langendoen N. J. Epithelial cell kinetics in the descending colon of the rat. Virchows Arch B Cell Pathol Incl Mol Pathol. 1979 May 4;30(1):85–94. doi: 10.1007/BF02889093. [DOI] [PubMed] [Google Scholar]
  22. Stragand J. J., Hagemann R. F. An iron requirement for the synchronous progression of colonic cells following fasting and refeeding. Cell Tissue Kinet. 1978 Sep;11(5):513–518. doi: 10.1111/j.1365-2184.1978.tb00823.x. [DOI] [PubMed] [Google Scholar]
  23. Stragand J. J., Hagemann R. F. Dietary influence on colonic cell renewal. Am J Clin Nutr. 1977 Jun;30(6):918–923. doi: 10.1093/ajcn/30.6.918. [DOI] [PubMed] [Google Scholar]
  24. Troughton W. D., Trier J. S. Paneth and goblet cell renewal in mouse duodenal crypts. J Cell Biol. 1969 Apr;41(1):251–268. doi: 10.1083/jcb.41.1.251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wiernick G., Perrins D. The radiosensitivity of a mesenchymal tissue. The pericryptal fibroblast sheath in the human rectal mucosa. Br J Radiol. 1975 May;48(569):382–389. doi: 10.1259/0007-1285-48-569-382. [DOI] [PubMed] [Google Scholar]
  26. Wright N. A., Al-Dewachi H. S., Appleton D. R., Watson A. J. Cell population kinetics in the rat jejunal crypt. Cell Tissue Kinet. 1975 Jul;8(4):361–368. doi: 10.1111/j.1365-2184.1975.tb01500.x. [DOI] [PubMed] [Google Scholar]
  27. Zajicek G. The intestinal proliferon. J Theor Biol. 1977 Aug 7;67(3):515–521. doi: 10.1016/0022-5193(77)90053-4. [DOI] [PubMed] [Google Scholar]

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