Skip to main content
PMC home page
Gut logoLink to Gut
. 1981 Dec;22(12):985–991. doi: 10.1136/gut.22.12.985

Immunohistochemical analysis of HLA (A, B, C) antigens in liver disease using a monoclonal antibody.

C Barbatis, J Woods, J A Morton, K A Fleming, A McMichael, J O McGee
PMCID: PMC1419485  PMID: 7033057

Abstract

The distribution of HLA class I antigens was studied in 42 liver biopsies and eight necropsies by an immunoperoxidase technique employing a monoclonal antibody which reacts with the heavy chains of class I (A, B, C) HLA antigens. In normal liver HLA class I antigens could not be detected on hepatocyte cell membranes or cytoplasm; these antigens were present on the cell membrane of bile duct epithelium, on sinusoidal lining cells, fibroblasts, and blood vessel endothelium. However, in all patients with acute alcoholic hepatitis, most cases of primary biliary cirrhosis and some cases of chronic active hepatitis HLA class I antigens were detectable focally or diffusely on the cell membrane of hepatocytes; in two cases of acute viral hepatitis (non-A, non-B) HLA class I antigens were present in granular form in the cytoplasm of all hepatocytes. These findings may be relevant to the prolonged survival of liver allografts in man and other species and in the pathogenesis of some liver diseases.

Full text

PDF
985

Images in this article

986Fig. 1
on p.986
987Fig. 2
on p.987
988Fig. 3
on p.988
988Fig. 4
on p.988
989Fig. 5
on p.989

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen F. H., Jr Linkage of HL-A and GBG. Vox Sang. 1974;27(4):382–384. doi: 10.1111/j.1423-0410.1974.tb02433.x. [DOI] [PubMed] [Google Scholar]
  2. Alper C. A. Inherited structural polymorphism in human C2: evidence for genetic linkage between C2 and Bf. J Exp Med. 1976 Oct 1;144(4):1111–1115. doi: 10.1084/jem.144.4.1111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Amos D. B., Kostyu D. D. HLA--a central immunological agency of man. Adv Hum Genet. 1980;10:137-208, 385-6. doi: 10.1007/978-1-4615-8288-5_2. [DOI] [PubMed] [Google Scholar]
  4. Barnstable C. J., Jones E. A., Crumpton M. J. Isolation, structure and genetics of HLA-A, -B, -C and -DRw (Ia) antigens. Br Med Bull. 1978 Sep;34(3):241–246. doi: 10.1093/oxfordjournals.bmb.a071504. [DOI] [PubMed] [Google Scholar]
  5. Berah M., Hors J., Dausset J. A study of HL-A antigens in human organs. Transplantation. 1970 Mar;9(3):185–192. doi: 10.1097/00007890-197003000-00001. [DOI] [PubMed] [Google Scholar]
  6. Brown G., Biberfeld P., Christensson B., Mason D. Y. The distribution of HLA on human lymphoid, bone marrow and peripheral blood cells. Eur J Immunol. 1979 Apr;9(4):272–275. doi: 10.1002/eji.1830090405. [DOI] [PubMed] [Google Scholar]
  7. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  8. Calne R. Y., McMaster P., Portmann B., Wall W. J., Williams R. Observations on preservation, bile drainage and rejection in 64 human orthotopic liver allografts. Ann Surg. 1977 Sep;186(3):282–290. doi: 10.1097/00000658-197709000-00006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dickmeiss E., Soeberg B., Svejgaard A. Human cell-mediated cytotoxicity against modified target cells is restricted by HLA. Nature. 1977 Dec 8;270(5637):526–528. doi: 10.1038/270526a0. [DOI] [PubMed] [Google Scholar]
  10. Fleming K. A., McMichael A., Morton J. A., Woods J., McGee J. O. Distribution of HLA class 1 antigens in normal human tissue and in mammary cancer. J Clin Pathol. 1981 Jul;34(7):779–784. doi: 10.1136/jcp.34.7.779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gomard E., Duprez V., Henin Y., Levy J. P. H-2 region product as determinant in immune cytolysis of syngeneic tumour cells by anti-MSV T lymphocytes. Nature. 1976 Apr 22;260(5553):707–709. doi: 10.1038/260707a0. [DOI] [PubMed] [Google Scholar]
  12. Jones J. V., Cowan J. D., Cumming J. HL-A antigens on glomeruli. Demonstration by an immunofluorescent technique. Transplantation. 1972 Dec;14(6):798–802. doi: 10.1097/00007890-197212000-00023. [DOI] [PubMed] [Google Scholar]
  13. Klein J., Hauptfeld V. Ia antigens: their serology, molecular relationships, and their role in allograft reactions. Transplant Rev. 1976;30:83–100. [PubMed] [Google Scholar]
  14. Klein J. The major histocompatibility complex of the mouse. Science. 1979 Feb 9;203(4380):516–521. doi: 10.1126/science.104386. [DOI] [PubMed] [Google Scholar]
  15. Kreth H. W., ter Meulen V., Eckert G. Demonstration of HLA restricted killer cells in patients with acute measles. Med Microbiol Immunol. 1979 Jan 24;165(4):203–214. doi: 10.1007/BF02152920. [DOI] [PubMed] [Google Scholar]
  16. Mattiuz P. L., Massobrio M., Richiardi P. Espressione degli antigeni del sistema HL-A sulle cellule fetali. Minerva Ginecol. 1973 Jan;25(1):8–13. [PubMed] [Google Scholar]
  17. McMichael A. J. HLA restriction of human cytotoxic T cells. Springer Semin Immunopathol. 1980 May;3(1):3–22. doi: 10.1007/BF00199923. [DOI] [PubMed] [Google Scholar]
  18. McMichael A. J., Ting A., Zweerink H. J., Askonas B. A. HLA restriction of cell-mediated lysis of influenza virus-infected human cells. Nature. 1977 Dec 8;270(5637):524–526. doi: 10.1038/270524a0. [DOI] [PubMed] [Google Scholar]
  19. Moss D. J., Rickinson A. B., Pope J. H. Long-term T-cell-mediated immunity to Epstein-Barr virus in man. III. Activation of cytotoxic T cells in virus-infected leukocyte cultures. Int J Cancer. 1979 May 15;23(5):618–625. doi: 10.1002/ijc.2910230506. [DOI] [PubMed] [Google Scholar]
  20. Seigler H. F., Metzgar R. S. Embryonic development of human transplantation antigens. Transplantation. 1970 May;9(5):478–486. doi: 10.1097/00007890-197005000-00007. [DOI] [PubMed] [Google Scholar]
  21. Shearer G. M., Schmitt-Verhulst A. M. Major histocompatibility complex restricted cell-mediated immunity. Adv Immunol. 1977;25:55–91. doi: 10.1016/s0065-2776(08)60931-1. [DOI] [PubMed] [Google Scholar]
  22. Shreffler D. C. The S region of the mouse major histocompatibility complex (H-2): genetic variation and functional role in complement system. Transplant Rev. 1976;32:140–167. doi: 10.1111/j.1600-065x.1976.tb00232.x. [DOI] [PubMed] [Google Scholar]
  23. Starzl T. E., Porter K. A., Putnam C. W., Schroter G. P., Halgrimson C. G., Weil R., 3rd, Hoelscher M., Reid H. A. Orthotopic liver transplantation in ninety-three patients. Surg Gynecol Obstet. 1976 Apr;142(4):487–505. [PMC free article] [PubMed] [Google Scholar]
  24. Sybesma J. P., Kater L., Borst-Eilers E., de Planque B. A., van Soelen T., Tuit G. HLA antigens in kidney tissue. Localisation by means of immunofluorescence technique. Transplantation. 1974 Jun;17(6):576–579. doi: 10.1097/00007890-197406000-00005. [DOI] [PubMed] [Google Scholar]
  25. Tursz T., Fridman W. H., Senik A., Tsapis A., Fellous M. Human virus-infected target cells lacking HLA antigens resist specific T-lymphocyte cytolysis. Nature. 1977 Oct 27;269(5631):806–808. doi: 10.1038/269806a0. [DOI] [PubMed] [Google Scholar]
  26. Wernet P. Human Ia-type alloantigens: methods of detection, aspects of chemistry and biology, markers for disease states. Transplant Rev. 1976;30:271–298. doi: 10.1111/j.1600-065x.1976.tb00223.x. [DOI] [PubMed] [Google Scholar]
  27. Williams K. A., Hart D. N., Fabre J. W., Morris P. J. Distribution and quantitation of HLA-ABC and DR (Ia) antigens on human kidney and other tissues. Transplantation. 1980 Apr;29(4):274–279. doi: 10.1097/00007890-198004000-00002. [DOI] [PubMed] [Google Scholar]
  28. Zinkernagel R. M., Doherty P. C. Immunological surveillance against altered self components by sensitised T lymphocytes in lymphocytic choriomeningitis. Nature. 1974 Oct 11;251(5475):547–548. doi: 10.1038/251547a0. [DOI] [PubMed] [Google Scholar]

Articles from Gut are provided here courtesy of BMJ Publishing Group

RESOURCES