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. 1982 Mar;23(3):221–225. doi: 10.1136/gut.23.3.221

Liver membrane antibodies in alcoholic liver disease: 1. prevalence and immunoglobulin class.

A D Burt, R S Anthony, W S Hislop, I A Bouchier, R N MacSween
PMCID: PMC1419630  PMID: 7040177

Abstract

Using an indirect immunofluorescence technique liver membrane antibodies of IgG and IgA class have been demonstrated in a statistically significant proportion of sera from patients with alcoholic hepatitis and alcoholic cirrhosis. IgG and IgA class antibodies were found respectively in 23 and 25% of 48 patients with alcoholic hepatitis, in 27 and 33% of 84 with active cirrhosis, and 67 and 58% of 12 with inactive cirrhosis. These results provide evidence of a humoral immune response in alcoholic liver disease which is directed against, as yet undefined, liver-cell membrane antigens.

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Selected References

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  1. Behrens U. J., Paronetto F. Studies on "liver-specific" antigens. I. Evaluation of the liver specificity of "LSP" and "LP-2". Gastroenterology. 1979 Nov;77(5):1045–1052. [PubMed] [Google Scholar]
  2. Behrens U. J., Vernace S., Paronetto F. Studies on "liver-specific" antigens. II. Detection of serum antibodies to liver and kidney cell membrane antigens in patients with chronic liver disease. Gastroenterology. 1979 Nov;77(5):1053–1061. [PubMed] [Google Scholar]
  3. Hopf U., Meyer zum Büschenfelde K. H., Arnold W. Detection of a liver-membrane autoantibody in HBsAg-negative chronic active hepatitis. N Engl J Med. 1976 Mar 11;294(11):578–582. doi: 10.1056/NEJM197603112941103. [DOI] [PubMed] [Google Scholar]
  4. Hopf U., Meyer zum Büschenfelde K. H., Dierich M. P. Demonstration of binding sites for IgG Fc and the third complement component (C3) on isolated hepatocytes. J Immunol. 1976 Aug;117(2):639–645. [PubMed] [Google Scholar]
  5. Hsu S. M., Hsu P. L. Demonstration of IgA and secretory component in human hepatocytes. Gut. 1980 Nov;21(11):985–989. doi: 10.1136/gut.21.11.985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jeejeebhoy K. N., Ho J., Greenberg G. R., Phillips M. J., Bruce-Robertson A., Sodtke U. Albumin, fibrinogen and transferrin synthesis in isolated rat hepatocyte suspensions. A model for the study of plasma protein synthesis. Biochem J. 1975 Jan;146(1):141–155. doi: 10.1042/bj1460141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Leevy C. M., Chen T., Luisada-Opper A., Kanagasundaram N., Zetterman R. Liver disease of the alcoholic: role of immunologic abnormalities in pathogenesis, recognition, and treatment. Prog Liver Dis. 1976;5:516–530. [PubMed] [Google Scholar]
  9. Lesko L., Donlon M., Marinetti G. V., Hare J. D. A rapid method for the isolation of rat liver plasma membranes using an aqueous two-phase polymer system. Biochim Biophys Acta. 1973 Jun 22;311(2):173–179. doi: 10.1016/0005-2736(73)90264-2. [DOI] [PubMed] [Google Scholar]
  10. Lieber C. S. Alcohol, protein metabolism, and liver injury. Gastroenterology. 1980 Aug;79(2):373–390. [PubMed] [Google Scholar]
  11. MacSween R. N., Anthony R. S., Farquharson M. Antibodies to alcohol-altered hepatocytes in patients with alcoholic liver disease. Lancet. 1981 Oct 10;2(8250):803–804. doi: 10.1016/s0140-6736(81)90205-1. [DOI] [PubMed] [Google Scholar]
  12. Manns M., Meyer zum Büschenfelde K. H., Hess G. Autoantibodies against liver-specific membrane lipoprotein in acute and chronic liver diseases: studies on organ-, species-, and disease-specificity. Gut. 1980 Nov;21(11):955–961. doi: 10.1136/gut.21.11.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Manns M., Meyer zum Büschenfelde K. H., Hütteroth T., Hopf U. The liver specific protein: evidence for species-specific and non-species-specific determinants. J Clin Lab Immunol. 1980 Jan;3(1):9–13. [PubMed] [Google Scholar]
  14. McFarlane I. G., Wojcicka B. M., Williams R. Antigens of the human liver. Clin Exp Immunol. 1980 Apr;40(1):1–7. [PMC free article] [PubMed] [Google Scholar]
  15. McFarlane I. G., Wojcicka B. M., Zucker G. M., Eddleston A. L., Williams R. Purification and characterization of human liver-specific membrane lipoprotein (LSP). Clin Exp Immunol. 1977 Mar;27(3):381–390. [PMC free article] [PubMed] [Google Scholar]
  16. Meyer zum Büschenfelde K. H., Manns M., Hütteroth T. H., Hopf U., Arnold W. LM-Ag and LSP--two different target antigens involved in the immunopathogenesis of chronic active hepatitis? Clin Exp Immunol. 1979 Aug;37(2):205–212. [PMC free article] [PubMed] [Google Scholar]
  17. Meyer zum Büschenfelde, Miescher P. A. Liver specific antigens. Purification and characterization. Clin Exp Immunol. 1972 Jan;10(1):89–102. [PMC free article] [PubMed] [Google Scholar]
  18. Morgan M. Y., Ross M. G., Ng C. M., Adams D. M., Thomas H. C., Sherlock S. HLA-B8, immunoglobulins, and antibody responses in alcohol-related liver disease. J Clin Pathol. 1980 May;33(5):488–492. doi: 10.1136/jcp.33.5.488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. NISONOFF A., WISSLER F. C., LIPMAN L. N., WOERNLEY D. L. Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulfide bonds. Arch Biochem Biophys. 1960 Aug;89:230–244. doi: 10.1016/0003-9861(60)90049-7. [DOI] [PubMed] [Google Scholar]
  20. Nasu H., Chia D. S., Knutson D. W., Barnett E. V. Naturally occurring human antibodies to the F(ab')2 portion of IgG. Clin Exp Immunol. 1980 Nov;42(2):378–386. [PMC free article] [PubMed] [Google Scholar]
  21. Steele E. J., Rowley D. The mechanism of complement fixation by rabbit F(ab')2: properties of the bactericidal reaction. Immunochemistry. 1977 May;14(5):319–324. doi: 10.1016/0019-2791(77)90230-0. [DOI] [PubMed] [Google Scholar]
  22. Tage-Jensen U., Arnold W., Dietrichson O., Hardt F., Hopf U., Meyer Zum Büschenfelde K. H., Nielsen J. O. Liver-cell-membrane autoantibody specific for inflammatory liver diseases. Br Med J. 1977 Jan 22;1(6055):206–208. doi: 10.1136/bmj.1.6055.206. [DOI] [PMC free article] [PubMed] [Google Scholar]

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