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. 1982 Jun;23(6):498–506. doi: 10.1136/gut.23.6.498

Features of small intestinal pathology (epithelial cell kinetics, intraepithelial lymphocytes, disaccharidases) in a primary Giardia muris infection.

J Gillon, D Al Thamery, A Ferguson
PMCID: PMC1419712  PMID: 7076025

Abstract

In an attempt to correlate host and parasite-related events occurring during the course of a primary Giardia infection in the mouse we have measured epithelial cell kinetics, enzymes, and intraepithelial lymphocytes at different stages of the infection. New methods were developed for the accurate measurement of parasite numbers and distribution within the gut. In jejunum a modest decrease in villus length and intraepithelial lymphocytes at week 1 preceded a pronounced disaccharidase deficiency at week 2, the time of maximum trophozoite numbers, whereas crypt lengthening and increased cell production became maximal at week 3. As trophozoite numbers fell the intraepithelial lymphocyte count and disaccharidase values rose. With the exception of the intraepithelial lymphocyte count, which followed the same pattern as in jejunum but two weeks later, the changes seen in the ileum were the opposite of those in jejunum, suggesting rapid ileal adaptation. The results indicate that the disaccharidase deficiency associated with giardiasis is likely to represent a direct effect of the parasite on the brush border rather than enterocyte immaturity, whereas the intraepithelial lymphocyte response reflects host immunity to the parasite. Profound adaptive changes occur throughout the small intestine in response to a relatively localised insult.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barbieri D., De Brito T., Hoshino S., Nascimento O. B., Martins Campos J. V., Quarentei G., Marcondes E. Giardiasis in childhood. Absorption tests and biochemistry, histochemistry, light and electron microscopy of jejunal mucosa. Arch Dis Child. 1970 Aug;45(242):466–472. doi: 10.1136/adc.45.242.466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clarke R. M. Mucosal architecture and epithelial cell production rate in the small intestine of the albino rat. J Anat. 1970 Nov;107(Pt 3):519–529. [PMC free article] [PubMed] [Google Scholar]
  3. Dahlqvist A. Assay of intestinal disaccharidases. Anal Biochem. 1968 Jan;22(1):99–107. doi: 10.1016/0003-2697(68)90263-7. [DOI] [PubMed] [Google Scholar]
  4. Duncombe V. M., Bolin T. D., Davis A. E., Cummins A. G., Crouch R. L. Histopathology in giardiasis: a correlation with diarrhoea. Aust N Z J Med. 1978 Aug;8(4):392–396. doi: 10.1111/j.1445-5994.1978.tb04908.x. [DOI] [PubMed] [Google Scholar]
  5. Ferguson A. Intraepithelial lymphocytes of the small intestine. Gut. 1977 Nov;18(11):921–937. doi: 10.1136/gut.18.11.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ferguson A., McClure J. P., Townley R. R. Intraepithelial lymphocyte counts in small intestinal biopsies from children with diarrhoea. Acta Paediatr Scand. 1976 Sep;65(5):541–546. doi: 10.1111/j.1651-2227.1976.tb04929.x. [DOI] [PubMed] [Google Scholar]
  7. Ferguson A., Murray D. Quantitation of intraepithelial lymphocytes in human jejunum. Gut. 1971 Dec;12(12):988–994. doi: 10.1136/gut.12.12.988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jennings W., Rowland R., Hecker R., Gibson G. E., Fitch R. J., Reid D. P. The significance of lowered jejunal disaccharidase levels. Aust N Z J Med. 1976 Dec;6(6):556–560. doi: 10.1111/j.1445-5994.1976.tb03994.x. [DOI] [PubMed] [Google Scholar]
  9. MacDonald T. T., Ferguson A. Hypersensitivity reactions in the small intestine. III. The effects of allograft rejection and of graft-versus-host disease on epithelial cell kinetics. Cell Tissue Kinet. 1977 Jul;10(4):301–312. [PubMed] [Google Scholar]
  10. MacDonald T. T., Ferguson A. Small intestinal epithelial cell kinetics and protozoal infection in mice. Gastroenterology. 1978 Mar;74(3):496–500. [PubMed] [Google Scholar]
  11. Mowat A. M., Ferguson A. Hypersensitivity reactions in the small intestine. 6. Pathogenesis of the graft-versus-host reaction in the small intestinal mucosa of the mouse. Transplantation. 1981 Sep;32(3):238–243. doi: 10.1097/00007890-198109000-00011. [DOI] [PubMed] [Google Scholar]
  12. Nair K. V., Gillon J., Ferguson A. Corticosteroid treatment increases parasite numbers in murine giardiasis. Gut. 1981 Jun;22(6):475–480. doi: 10.1136/gut.22.6.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Owen R. L., Nemanic P. C., Stevens D. P. Ultrastructural observations on giardiasis in a murine model. I. Intestinal distribution, attachment, and relationship to the immune system of Giardia muris. Gastroenterology. 1979 Apr;76(4):757–769. [PubMed] [Google Scholar]
  14. Rey J., Schmitz J., Rey F., Jos J. Cellular differentiation and enzymatic deficits. Lancet. 1971 Jul 24;2(7717):218–218. doi: 10.1016/s0140-6736(71)90932-9. [DOI] [PubMed] [Google Scholar]
  15. Roberts-Thomson I. C., Mitchell G. F. Giardiasis in mice. I. Prolonged infections in certain mouse strains and hypothymic (nude) mice. Gastroenterology. 1978 Jul;75(1):42–46. [PubMed] [Google Scholar]
  16. Roberts-Thomson I. C., Stevens D. P., Mahmoud A. A., Warren K. S. Giardiasis in the mouse: an animal model. Gastroenterology. 1976 Jul;71(1):57–61. [PubMed] [Google Scholar]
  17. TAKANO J., YARDLEY J. H. JEJUNAL LESIONS IN PATIENTS WITH GIARDIASIS AND MALABSORPTION. AN ELECTRON MICROSCOPIC STUDY. Bull Johns Hopkins Hosp. 1965 Jun;116:413–429. [PubMed] [Google Scholar]
  18. Tandon B. N., Tandon R. K., Satpathy B. K., Shriniwas Mechanism of malabsorption in giardiasis: a study of bacterial flora and bile salt deconjugation in upper jejunum. Gut. 1977 Mar;18(3):176–181. doi: 10.1136/gut.18.3.176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tomkins A. M., Wright S. G., Drasar B. S., James W. P. Bacterial colonization of jejunal mucosa in giardiasis. Trans R Soc Trop Med Hyg. 1978;72(1):33–36. doi: 10.1016/0035-9203(78)90294-8. [DOI] [PubMed] [Google Scholar]
  20. Wehman H. J., Lifshitz F., Teichberg S. Effects of enteric microbial overgrowth on small intestinal ultrastructure in the rat. Am J Gastroenterol. 1978 Sep;70(3):249–258. [PubMed] [Google Scholar]
  21. Wright S. G., Tomkins A. M. Quantification of the lymphocytic infiltrate in jejunal epithelium in giardiasis. Clin Exp Immunol. 1977 Sep;29(3):408–412. [PMC free article] [PubMed] [Google Scholar]
  22. Wright S. G., Tomkins A. M. Quantitative histology in giardiasis. J Clin Pathol. 1978 Aug;31(8):712–716. doi: 10.1136/jcp.31.8.712. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wright S. G., Tomkins A. M., Ridley D. S. Giardiasis: clinical and therapeutic aspects. Gut. 1977 May;18(5):343–350. doi: 10.1136/gut.18.5.343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. YARDLEYJHTAKANO J., HENDRIX T. R. EPITHELIAL AND OTHER MUCOSAL LESIONS OF THE JEJUNUM IN GIARDIASIS. JEJUNAL BIOPSY STUDIES. Bull Johns Hopkins Hosp. 1964 Nov;115:389–406. [PubMed] [Google Scholar]

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