Skip to main content
NCBI home page
Gut logoLink to Gut
. 1983 Oct;24(10):935–939. doi: 10.1136/gut.24.10.935

Effect of cysteamine on gastroduodenal mucosal histamine in rat.

S Boesby, W K Man, R Mendez-Diaz, J Spencer
PMCID: PMC1420139  PMID: 6618272

Abstract

Cysteamine administration to rats is followed by a high incidence of peptic ulceration. The aim of the present study was to investigate the effect of cysteamine on gastric and duodenal mucosal histamine and gastric mucosal histamine formation capacity. After a four hour fast, cysteamine in doses of 50, 100, 200, 300, 400, and 500 mg/kg bodyweight was injected subcutaneously to male Wistar rats; saline injection was used as control. After 24 hours the animals were killed; the stomach and duodenum were removed and examined for ulceration. Mucosal biopsies were taken for histamine studies. Gastric and duodenal ulceration tended to appear with increasing incidence with higher doses. A direct correlation was found between the dose of cysteamine and gastric mucosal histamine (p less than 0.02), and duodenal mucosal histamine (p less than 0.05). Further, a direct relationship was found between gastric mucosal histidine decarboxylase activity and the dose of cysteamine (p less than 0.05). Gastric mucosal histamine and histidine decarboxylase activity showed a direct correlation (p less than 0.001). Gastric and duodenal mucosal histamine and gastric mucosal histamine formation capacity were higher in rats with ulcers than in controls and rats without ulcers. In rat, cysteamine induces dose related changes in mucosal histamine and histidine decarboxylase activity. These changes are related to ulcer formation; histamine may be involved in the pathophysiology of cysteamine induced ulcer formation.

Full text

PDF
935

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beaven M. A., Horakova Z., Severs W. B., Brodie B. B. Selective labeling of histamine in rat gastric mucosa: application to measurement of turnover rate. J Pharmacol Exp Ther. 1968 Jun;161(2):320–328. [PubMed] [Google Scholar]
  2. Beraha N., Silveira M., Man W., Silcocks P. B., Spencer J. Catecholamines and experimental stress ulcer: morphological and biochemical changes in the gastric mucosa. Br J Surg. 1980 Sep;67(9):624–628. doi: 10.1002/bjs.1800670906. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ishii Y., Fujii Y., Homma M. Gastric acid stimulating action of cysteamine in the rat. Eur J Pharmacol. 1976 Apr;36(2):331–336. doi: 10.1016/0014-2999(76)90086-8. [DOI] [PubMed] [Google Scholar]
  4. KAHLSON G., ROSENGREN E., SVAHN D., THUNBERG R. MOBILIZATION AND FORMATION OF HISTAMINE IN THE GASTRIC MUCOSA AS RELATED TO ACID SECRETION. J Physiol. 1964 Nov;174:400–416. doi: 10.1113/jphysiol.1964.sp007494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kirkegaard P., Petersen S., Skov Olsen P., Poulsen S. S., Christiansen J. The mechanism of gastrin release in cysteamine-induced duodenal ulcer. Scand J Gastroenterol. 1982 Aug;17(5):609–612. doi: 10.3109/00365528209181066. [DOI] [PubMed] [Google Scholar]
  6. Kirkegaard P., Poulsen S. S., Halse C., Loud F. B., Skov Olsen P., Christiansen J. The effect of cysteamine on the Brunner gland secretion in the rat. Scand J Gastroenterol. 1981;16(1):93–96. [PubMed] [Google Scholar]
  7. Kirkegaard P., Poulsen S. S., Loud F. B., Halse C., Christiansen J. Cysteamine-induced duodenal ulcer and acid secretion in the rat. Scand J Gastroenterol. 1980;15(5):621–624. doi: 10.3109/00365528009182225. [DOI] [PubMed] [Google Scholar]
  8. Lichtenberger L. M., Szabo S., Reynolds E. S. Gastric emptying in the rat is inhibited by the duodenal ulcerogens, cysteamine and propionitrile. Gastroenterology. 1977 Nov;73(5):1072–1076. [PubMed] [Google Scholar]
  9. Lichtenberger L. M., Szabo S., Trier J. S. Duodenal ulcerogens, cysteamine and propionitrile, stimulate serum gastrin levels in the rat. Gastroenterology. 1977 Dec;73(6):1305–1308. [PubMed] [Google Scholar]
  10. Man W. K., Saunders J. H., Ingoldby C., Spencer J. Effect of cimetidine on the amounts of histamine in the gastric mucosa of patients with gastric or duodenal ulcers. Gut. 1981 Nov;22(11):923–926. doi: 10.1136/gut.22.11.923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Man W. K., Saunders J. H., Ingoldby C., Spencer J. Effect of pentagastrin on histamine output from the stomach in patients with duodenal ulcer. Gut. 1981 Nov;22(11):916–922. doi: 10.1136/gut.22.11.916. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Man W. K., Silcocks P. B., Waldes R., Spencer J. Histology of experimental stress ulcer: the effect of cimetidine on adrenaline-induced gastric lesions in the rabbit. Br J Exp Pathol. 1981 Aug;62(4):411–418. [PMC free article] [PubMed] [Google Scholar]
  13. Poulsen S. S., Kirkegaard P., Olsen P. S., Jensen K. K., Christiansen J. Role of delayed gastric emptying in the pathogenesis of cysteamine-induced duodenal ulcer in the rat. Scand J Gastroenterol. 1982 Apr;17(3):325–330. doi: 10.3109/00365528209182062. [DOI] [PubMed] [Google Scholar]
  14. Poulsen S. S., Kirkegaard P., Skov Olsen P., Christiansen J. Brunner's glands of the rat during cysteamine ulceration. Scand J Gastroenterol. 1981;16(4):459–464. doi: 10.3109/00365528109181998. [DOI] [PubMed] [Google Scholar]
  15. Rhode H., Lorenz W., Troidl H., Reimann H. J., Häfner G., Weber D. Histamine and peptic ulcer: influence of sample-taking on the precision and accuracy of fluorometric histamine assay in biopsies of human gastric mucosa. Agents Actions. 1980 Apr;10(1 Pt 2):175–185. doi: 10.1007/BF02024207. [DOI] [PubMed] [Google Scholar]
  16. Selye H., Szabo S. Experimental model for production of perforating duodenal ulcers by cysteamine in the rat. Nature. 1973 Aug 17;244(5416):458–459. doi: 10.1038/244458a0. [DOI] [PubMed] [Google Scholar]
  17. Shaff R. E., Beaven M. A. Increased sensitivity of the enzymatic isotopic assay of histamine: measurement of histamine in plasma and serum. Anal Biochem. 1979 Apr 15;94(2):425–430. doi: 10.1016/0003-2697(79)90385-3. [DOI] [PubMed] [Google Scholar]
  18. Szabo S., Haith L. R., Jr, Reynolds E. S. Pathogenesis of duodenal ulceration produced by cysteamine or propionitrile: influence of vagotomy, sympathectomy, histamine depletion, H-2 receptor antagonists and hormones. Dig Dis Sci. 1979 Jun;24(6):471–477. doi: 10.1007/BF01299831. [DOI] [PubMed] [Google Scholar]
  19. Tamaki H., Onoda Y., Kashida T. Gastric secretion and duodenal ulcer formation induced by cysteamine in rats. Jpn J Pharmacol. 1978 Aug;28(4):647–649. doi: 10.1254/jjp.28.647. [DOI] [PubMed] [Google Scholar]
  20. Taylor K. M., Snyder S. H. Isotopic microassay of histamine, histidine, histidine decarboxylase and histamine methyltransferase in brain tissue. J Neurochem. 1972 May;19(5):1343–1358. doi: 10.1111/j.1471-4159.1972.tb01459.x. [DOI] [PubMed] [Google Scholar]

Articles from Gut are provided here courtesy of BMJ Publishing Group

RESOURCES