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. Author manuscript; available in PMC: 2007 Jan 30.
Published in final edited form as: Vaccine. 2005 Aug 24;24(5):671–682. doi: 10.1016/j.vaccine.2005.08.038

Table 1.

DC transduced by Ad-FBAP-GFP exhibit an enhanced level of phenotypic maturation, as compared to untransduced DC in the same culture.

DC Marker Receptor Targeting GFP+ Cells GFP− Cells
CD40 Untargeted 4.16 3.32
Isotype 3.80 3.01
Anti-DC-SIGN 3.73 3.11
3JCLI4 3.63 3.34
FNfn10 3.67 2.92
Chemerin (FAAS) 4.11 3.29
Chemerin (FAFS)
4.34
3.5
CD86 Untargeted 142 82
Isotype 138 86
Anti-DC-SIGN 152 95
3JCLI4 157 102
FNfn10 105 63
Chemerin (FAAS) 126 74
Chemerin (FAFS)
140
85
Class II Untargeted 213 127
Isotype 240 131
Anti-DC-SIGN 187 120
3JCLI4 253 154
FNfn10 188 112
Chemerin (FAAS) 202 114
Chemerin (FAFS) 224 140

DC were transduced with Ad-FBAP-GFP targeted with the indicated molecules (see Fig. 2 legend). 48h later, cells were harvested and subjected to flow cytometric analysis using antibodies against CD40, CD86, and MHC Class II. Analysis of staining was then performed separately for gated GFP+ and GFP− cells within the same culture (i.e., cells that were transduced by the vector as well as “bystander” cells). Data shown represent mean fluorescence intensity values from three replicate measurements, taken from a single experiment. The data are representative of results from 3 independent experiments.