Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Apr;12(4):631–643. doi: 10.1261/rna.2226106

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © Copyright 2006 by RNA Society

PMC Copyright notice

FIGURE 5. — Cellular localization of AtCyp59. (A) Localization of AtCyp59-GFP fusion protein and GFP alone in transiently transformed tobacco protoplasts. Dashed line delineates the shape of protoplasts. Arrows point to nuclei. (B) Localization of AtCyp59-GFP fusion protein in transiently transformed Arabidopsis protoplasts. Single confocal image with corresponding differential interference contrast (DIC) image of whole cell is shown. Arrows point to nuclei. Protoplasts were analyzed 24 h after transformation by using Zeiss Axiovert epifluorescence microscope (A) or Leica TCS confocal microscope (B). Bars, 50 μm (A,B). (C) Cellular localization of AtCyp59 studied by cellular fractionation of Arabidopsis protoplasts transiently expressing AtCyp59-GFP or AtCyp59-HA. Western blots were analyzed with monoclonal antibodies against GFP or HA tags. The distribution of endogenous nuclear UBP1 protein was used to control quality of the fractionation procedure. (Lane 1) Total protein extracts (T), (lane 2) cytoplasmic fraction (C), (lane 3) nuclear fraction (N). (D) Localization of AtCyp59-HA deletions in transiently transformed Arabidopsis protoplasts as determined by cellular fractionation. Details are as in C.