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. 1992 May;76(1):103–109.

Human CD4+ T cells expressing CD45RA acquire the lymphokine gene expression of CD45RO+ T-helper cells after activation in vitro.

K Kristensson 1, C A Borrebaeck 1, R Carlsson 1
PMCID: PMC1421743  PMID: 1352764

Abstract

CD4+ T cells were separated into subpopulations according to their expression of different isoforms of the CD45R molecule, i.e. CD45RA and CD45RO. The separated cells were activated with staphylococcal enterotoxin A (SEA) in the presence of formalin fixed Raji cells. Each set of cells was activated twice with a 6-day interval, and the lymphokine gene expression during the first 3 days after initiation of each stimulation was followed by use of polymerase chain reaction (PCR) technology. The lymphokine messenger RNA (mRNA) profiles were found to differ between the subsets, since after the first stimulation the CD45RA+ cells produced mRNA encoding interleukin-2 (IL-2) and IL-1 alpha, whereas the CD45RO+ cells transcribed genes for IL-1 alpha, IL-2, IL-4, IL-5 and interferon-gamma (IFN-gamma). After 6 days of SEA stimulation both populations were mainly CD45RO reactive, and when restimulated displayed the lymphokine mRNA profile restricted to this subset. These results indicate that the CD45RA subset is a precursor of the CD45RO and further strengthen the hypothesis that the former cell population represents naive whereas the latter subset represents memory T cells within the CD4 subset.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akbar A. N., Terry L., Timms A., Beverley P. C., Janossy G. Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells. J Immunol. 1988 Apr 1;140(7):2171–2178. [PubMed] [Google Scholar]
  2. Bettens F., Walker C., Gauchat J. F., Gauchat D., Wyss T., Pichler W. J. Lymphokine gene expression related to CD4 T cell subset (CD45R/CDw29) phenotype conversion. Eur J Immunol. 1989 Sep;19(9):1569–1574. doi: 10.1002/eji.1830190908. [DOI] [PubMed] [Google Scholar]
  3. Brodin N. T., Jansson B., Hedlund G., Sjögren H. O. Use of a monoclonal rat anti-mouse Ig light chain (RAMOL-1) antibody reduces background binding in immunohistochemical and fluorescent antibody analysis. J Histochem Cytochem. 1989 Jul;37(7):1013–1024. doi: 10.1177/37.7.2499618. [DOI] [PubMed] [Google Scholar]
  4. Carlsson R., Dohlsten M., Boketoft A., Sjöquist J., Sjögren H. O. Staphylococcal protein A (SpA) does not induce production of interferon-gamma in human mononuclear blood cells. Cell Immunol. 1984 Jun;86(1):136–144. doi: 10.1016/0008-8749(84)90366-6. [DOI] [PubMed] [Google Scholar]
  5. Carlsson R., Fischer H., Sjögren H. O. Binding of staphylococcal enterotoxin A to accessory cells is a requirement for its ability to activate human T cells. J Immunol. 1988 Apr 15;140(8):2484–2488. [PubMed] [Google Scholar]
  6. Cerdan C., Martin Y., Brailly H., Courcoul M., Flavetta S., Costello R., Mawas C., Birg F., Olive D. IL-1 alpha is produced by T lymphocytes activated via the CD2 plus CD28 pathways. J Immunol. 1991 Jan 15;146(2):560–564. [PubMed] [Google Scholar]
  7. Clement L. T., Yamashita N., Martin A. M. The functionally distinct subpopulations of human CD4+ helper/inducer T lymphocytes defined by anti-CD45R antibodies derive sequentially from a differentiation pathway that is regulated by activation-dependent post-thymic differentiation. J Immunol. 1988 Sep 1;141(5):1464–1470. [PubMed] [Google Scholar]
  8. Dohlsten M., Hedlund G., Sjögren H. O., Carlsson R. Two subsets of human CD4+ T helper cells differing in kinetics and capacities to produce interleukin 2 and interferon-gamma can be defined by the Leu-18 and UCHL1 monoclonal antibodies. Eur J Immunol. 1988 Aug;18(8):1173–1178. doi: 10.1002/eji.1830180805. [DOI] [PubMed] [Google Scholar]
  9. Kristensson K., Dohlsten M., Fischer H., Ericsson P. O., Hedlund G., Sjögren H. O., Carlsson R. Phenotypical and functional differentiation of CD4+ CD45RA+ human T cells following polyclonal activation. Scand J Immunol. 1990 Sep;32(3):243–253. doi: 10.1111/j.1365-3083.1990.tb02917.x. [DOI] [PubMed] [Google Scholar]
  10. Lea T., Smeland E., Funderud S., Vartdal F., Davies C., Beiske K., Ugelstad J. Characterization of human mononuclear cells after positive selection with immunomagnetic particles. Scand J Immunol. 1986 Apr;23(4):509–519. doi: 10.1111/j.1365-3083.1986.tb03083.x. [DOI] [PubMed] [Google Scholar]
  11. Morimoto C., Letvin N. L., Distaso J. A., Aldrich W. R., Schlossman S. F. The isolation and characterization of the human suppressor inducer T cell subset. J Immunol. 1985 Mar;134(3):1508–1515. [PubMed] [Google Scholar]
  12. Rothstein D. M., Sohen S., Daley J. F., Schlossman S. F., Morimoto C. CD4+CD45RA+ and CD4+CD45RA- T cell subsets in man maintain distinct function and CD45RA expression persists on a subpopulation of CD45RA+ cells after activation with Con A. Cell Immunol. 1990 Sep;129(2):449–467. doi: 10.1016/0008-8749(90)90220-l. [DOI] [PubMed] [Google Scholar]
  13. Salmon M., Kitas G. D., Bacon P. A. Production of lymphokine mRNA by CD45R+ and CD45R- helper T cells from human peripheral blood and by human CD4+ T cell clones. J Immunol. 1989 Aug 1;143(3):907–912. [PubMed] [Google Scholar]
  14. Sanders M. E., Makgoba M. W., Sharrow S. O., Stephany D., Springer T. A., Young H. A., Shaw S. Human memory T lymphocytes express increased levels of three cell adhesion molecules (LFA-3, CD2, and LFA-1) and three other molecules (UCHL1, CDw29, and Pgp-1) and have enhanced IFN-gamma production. J Immunol. 1988 Mar 1;140(5):1401–1407. [PubMed] [Google Scholar]
  15. Serra H. M., Krowka J. F., Ledbetter J. A., Pilarski L. M. Loss of CD45R (Lp220) represents a post-thymic T cell differentiation event. J Immunol. 1988 Mar 1;140(5):1435–1441. [PubMed] [Google Scholar]
  16. Smith S. H., Brown M. H., Rowe D., Callard R. E., Beverley P. C. Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. Immunology. 1986 May;58(1):63–70. [PMC free article] [PubMed] [Google Scholar]
  17. Takeuchi T., Rudd C. E., Schlossman S. F., Morimoto C. Induction of suppression following autologous mixed lymphocyte reaction; role of a novel 2H4 antigen. Eur J Immunol. 1987 Jan;17(1):97–103. doi: 10.1002/eji.1830170117. [DOI] [PubMed] [Google Scholar]
  18. Tartakovsky B., Kovacs E. J., Takacs L., Durum S. K. T cell clone producing an IL 1-like activity after stimulation by antigen-presenting B cells. J Immunol. 1986 Jul 1;137(1):160–166. [PubMed] [Google Scholar]
  19. Tedder T. F., Cooper M. D., Clement L. T. Human lymphocyte differentiation antigens HB-10 and HB-11. II. Differential production of B cell growth and differentiation factors by distinct helper T cell subpopulations. J Immunol. 1985 May;134(5):2989–2994. [PubMed] [Google Scholar]
  20. Zubiaga A. M., Muñoz E., Huber B. T. Production of IL-1 alpha by activated Th type 2 cells. Its role as an autocrine growth factor. J Immunol. 1991 Jun 1;146(11):3849–3856. [PubMed] [Google Scholar]

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