Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1993 Jan;78(1):142–146.

Increased proliferative response of lymphocytes from intestinal lymph during long chain fatty acid absorption.

S Miura 1, H Imaeda 1, H Shiozaki 1, N Ohkubo 1, H Tashiro 1, H Serizawa 1, M Tsuchiya 1, P Tso 1
PMCID: PMC1421772  PMID: 8436400

Abstract

The effect of long chain and medium chain fatty acid absorption on transport and mitogen-induced blast transformation of lymphocytes in intestinal lymphatics was investigated. Intestinal lymph was collected from mesenteric lymph duct cannulated rats maintained in Bollman's cage. Following the intraduodenal administration of oleic acid (long chain fatty acid) or octanoic acid (medium chain fatty acid), only oleic acid produced a significant increase in lymphocyte flux and enhanced proliferative response of lymphocyte in intestinal lymph, without significant alteration of lymphocyte subsets. These changes appeared to be closely correlated with the appearance of radiolabelled oleic acid. Absorption of a medium chain fatty acid, octanoic acid, most of which appeared to be transported to portal blood, did not produce a significant elevation of lymphocyte flux or increased proliferative response of lymphocyte in intestinal lymph. Pluronic L-81, which is a potent inhibitor of the intracellular formation and secretion of chylomicron, significantly attenuated the increased lymphocyte flux and suppressed the enhancement of lymphocyte responsiveness to PHA in intestinal lymph after stimulation by oleic acid administration. There is a possibility that lymphocyte transport and proliferative response in intestinal lymph during oleic acid absorption are closely related to the process of chylomicron formation and secretion to lymphatics in the intestinal mucosa.

Full text

PDF
142

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arai S., Yamane I., Tanno Y., Takishima T. Role of bovine serum albumin in blastoid transformation of lymphocytes by phytohemagglutinin. Proc Soc Exp Biol Med. 1977 Mar;154(3):444–448. doi: 10.3181/00379727-154-39690. [DOI] [PubMed] [Google Scholar]
  2. Beisel W. R. Single nutrients and immunity. Am J Clin Nutr. 1982 Feb;35(2 Suppl):417–468. doi: 10.1093/ajcn/35.2.417. [DOI] [PubMed] [Google Scholar]
  3. Curtiss L. K., Edgington T. S. The biologic activity of the immunoregulatory lipoprotein, LDL-In is independent of its free fatty acid content. J Immunol. 1981 Apr;126(4):1382–1386. [PubMed] [Google Scholar]
  4. Cuthbert J. A., Lipsky P. E. Immunoregulation by low density lipoproteins in man. Inhibition of mitogen-induced T lymphocyte proliferation by interference with transferrin metabolism. J Clin Invest. 1984 Apr;73(4):992–1003. doi: 10.1172/JCI111325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cuthbert J. A., Lipsky P. E. Lipoproteins may provide fatty acids necessary for human lymphocyte proliferation by both low density lipoprotein receptor-dependent and -independent mechanisms. J Biol Chem. 1989 Aug 15;264(23):13468–13474. [PubMed] [Google Scholar]
  6. Cuthbert J. A., Lipsky P. E. Modulation of human lymphocyte responses by low density lipoproteins (LDL): enhancement but not immunosuppression is mediated by LDL receptors. Proc Natl Acad Sci U S A. 1984 Jul;81(14):4539–4543. doi: 10.1073/pnas.81.14.4539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cuthbert J. A., Lipsky P. E. Promotion of human T lymphocyte activation and proliferation by fatty acids in low density and high density lipoproteins. J Biol Chem. 1986 Mar 15;261(8):3620–3627. [PubMed] [Google Scholar]
  8. De Deckere E. A., Verplanke C. J., Blonk C. G., Van Nielen W. G. Effects of type and amount of dietary fat on rabbit and rat lymphocyte proliferation in vitro. J Nutr. 1988 Jan;118(1):11–18. doi: 10.1093/jn/118.1.11. [DOI] [PubMed] [Google Scholar]
  9. Erickson K. L., Adams D. A., McNeill C. J. Dietary lipid modulation of immune responsiveness. Lipids. 1983 Jul;18(7):468–474. doi: 10.1007/BF02535787. [DOI] [PubMed] [Google Scholar]
  10. Erickson K. L. Dietary fat modulation of immune response. Int J Immunopharmacol. 1986;8(6):529–543. doi: 10.1016/0192-0561(86)90023-8. [DOI] [PubMed] [Google Scholar]
  11. Erickson K. L., McNeill C. J., Gershwin M. E., Ossmann J. B. Influence of dietary fat concentration and saturation on immune ontogeny in mice. J Nutr. 1980 Aug;110(8):1555–1572. doi: 10.1093/jn/110.8.1555. [DOI] [PubMed] [Google Scholar]
  12. Friend J. V., Lock S. O., Gurr M. I., Parish W. E. Effect of different dietary lipids on the immune responses of Hartley strain guinea pigs. Int Arch Allergy Appl Immunol. 1980;62(3):292–301. doi: 10.1159/000232525. [DOI] [PubMed] [Google Scholar]
  13. Goppelt M., Köhler L., Resch K. Functional role of lipid metabolism in activated T-lymphocytes. Biochim Biophys Acta. 1985 Mar 6;833(3):463–472. doi: 10.1016/0005-2760(85)90104-3. [DOI] [PubMed] [Google Scholar]
  14. Greenberger N. J., Rodgers J. B., Isselbacher K. J. Absorption of medium and long chain triglycerides: factors influencing their hydrolysis and transport. J Clin Invest. 1966 Feb;45(2):217–227. doi: 10.1172/JCI105334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gross R. L., Newberne P. M. Role of nutrition in immunologic function. Physiol Rev. 1980 Jan;60(1):188–302. doi: 10.1152/physrev.1980.60.1.188. [DOI] [PubMed] [Google Scholar]
  16. Gurr M. I. The role of lipids in the regulation of the immune system. Prog Lipid Res. 1983;22(4):257–287. doi: 10.1016/0163-7827(83)90007-3. [DOI] [PubMed] [Google Scholar]
  17. Kelley D. S., Nelson G. J., Serrato C. M., Schmidt P. C., Branch L. B. Effects of type of dietary fat on indices of immune status of rabbits. J Nutr. 1988 Nov;118(11):1376–1384. doi: 10.1093/jn/118.11.1376. [DOI] [PubMed] [Google Scholar]
  18. Kelly J. P., Parker C. W. Effects of arachidonic acid and other unsaturated fatty acids on mitogenesis in human lymphocytes. J Immunol. 1979 Apr;122(4):1556–1562. [PubMed] [Google Scholar]
  19. Locniskar M., Nauss K. M., Newberne P. M. The effect of quality and quantity of dietary fat on the immune system. J Nutr. 1983 May;113(5):951–961. doi: 10.1093/jn/113.5.951. [DOI] [PubMed] [Google Scholar]
  20. Marshall L. A., Johnston P. V. The influence of dietary essential fatty acids on rat immunocompetent cell prostaglandin synthesis and mitogen-induced blastogenesis. J Nutr. 1985 Dec;115(12):1572–1580. doi: 10.1093/jn/115.12.1572. [DOI] [PubMed] [Google Scholar]
  21. Meade C. J., Mertin J. Fatty acids and immunity. Adv Lipid Res. 1978;16:127–165. doi: 10.1016/b978-0-12-024916-9.50008-1. [DOI] [PubMed] [Google Scholar]
  22. Mertin J., Hughes D. Specific inhibitory action of polyunsaturated fatty acids on lymphocyte transformation induced by PHA and PPD. Int Arch Allergy Appl Immunol. 1975;48(2):203–210. doi: 10.1159/000231306. [DOI] [PubMed] [Google Scholar]
  23. Miura S., Sekizuka E., Nagata H., Oshio C., Minamitani H., Suematsu M., Suzuki M., Hamada Y., Kobayashi K., Asakura H. Increased lymphocyte transport by lipid absorption in rat mesenteric lymphatics. Am J Physiol. 1987 Nov;253(5 Pt 1):G596–G600. doi: 10.1152/ajpgi.1987.253.5.G596. [DOI] [PubMed] [Google Scholar]
  24. Steer H. W. An analysis of the lymphocyte content of rat lacteals. J Immunol. 1980 Oct;125(4):1845–1848. [PubMed] [Google Scholar]
  25. Tso P., Balint J. A., Bishop M. B., Rodgers J. B. Acute inhibition of intestinal lipid transport by Pluronic L-81 in the rat. Am J Physiol. 1981 Dec;241(6):G487–G497. doi: 10.1152/ajpgi.1981.241.6.G487. [DOI] [PubMed] [Google Scholar]
  26. Tso P., Drake D. S., Black D. D., Sabesin S. M. Evidence for separate pathways of chylomicron and very low-density lipoprotein assembly and transport by rat small intestine. Am J Physiol. 1984 Dec;247(6 Pt 1):G599–G610. doi: 10.1152/ajpgi.1984.247.6.G599. [DOI] [PubMed] [Google Scholar]
  27. Vitale J. J., Broitman S. A. Lipids and immune function. Cancer Res. 1981 Sep;41(9 Pt 2):3706–3710. [PubMed] [Google Scholar]
  28. Weyman C., Morgan S. J., Belin J., Smith A. D. Phytohaemagglutinin stimulation of human lymphocytes: effect of fatty acids on uridine uptake and phosphoglyceride fatty acid profile. Biochim Biophys Acta. 1977 Jan 24;496(1):155–166. doi: 10.1016/0304-4165(77)90123-4. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES