Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1993 Mar;78(3):341–348.

Structure and homology of human C1q receptor (collectin receptor).

R Malhotra 1, A C Willis 1, J C Jensenius 1, J Jackson 1, R B Sim 1
PMCID: PMC1421832  PMID: 8478019

Abstract

In this paper we report partial amino acid sequence for C1q receptor (C1qR). The N-terminal amino acid sequence of isolated C1qR and the sequences of peptides obtained by V8/trypsin digestion show a high degree of similarity to the cDNA-derived amino acid sequence of a human protein which was initially reported as a component of RoSSA and subsequently as calreticulin. This sequence in turn shows homology with Onchocerca volvulus antigen (RAL-1) and B50 murine melanoma antigen. A component of approximately 53,000 MW, isolated from human spleen, was found to have identical mobility on SDS-PAGE to C1qR and identical N-terminal sequence, but a different overall charge. Human antibodies from Sjögren's syndrome patients did not recognize C1qR, but showed positive reaction with the purified 53,000 MW component from spleen. Rabbit antibodies against denatured C1qR, in contrast, recognized both C1qR and the purified 53,000 MW component. The 53,000 MW spleen component thus has an identical N-terminal sequence to calreticulin, and to the reported RoSSA component, and is recognized by antibodies in Sjögren's syndrome sera. The data obtained indicate that C1qR and the reported calreticulin/RoSSA component are similar but not identical molecules, which belong to the same protein superfamily.

Full text

PDF
341

Images in this article

343Figure 1
on p.343
344Figure 2
on p.344
346Figure 5
on p.346

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dickler H. B., Kunkel H. G. Interaction of aggregated -globulin with B lymphocytes. J Exp Med. 1972 Jul 1;136(1):191–196. doi: 10.1084/jem.136.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Erdei A., Reid K. B. Characterization of C1q-binding material released from the membranes of Raji and U937 cells by limited proteolysis with trypsin. Biochem J. 1988 Oct 15;255(2):493–499. [PMC free article] [PubMed] [Google Scholar]
  3. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  4. Fraker P. J., Speck J. C., Jr Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril. Biochem Biophys Res Commun. 1978 Feb 28;80(4):849–857. doi: 10.1016/0006-291x(78)91322-0. [DOI] [PubMed] [Google Scholar]
  5. Gersten D. M., Bijwaard K. E., Law L. W., Hearing V. J. Homology of the B50 murine melanoma antigen to the Ro/SS-A antigen of human systemic lupus erythematosus and to calcium-binding proteins. Biochim Biophys Acta. 1990 Nov 14;1096(1):20–25. doi: 10.1016/0925-4439(90)90007-c. [DOI] [PubMed] [Google Scholar]
  6. Ghebrehiwet B. Functions associated with the C1q receptor. Behring Inst Mitt. 1989 Jul;(84):204–215. [PubMed] [Google Scholar]
  7. Kennedy T. E., Gawinowicz M. A., Barzilai A., Kandel E. R., Sweatt J. D. Sequencing of proteins from two-dimensional gels by using in situ digestion and transfer of peptides to polyvinylidene difluoride membranes: application to proteins associated with sensitization in Aplysia. Proc Natl Acad Sci U S A. 1988 Sep;85(18):7008–7012. doi: 10.1073/pnas.85.18.7008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kölble K., Rukavina V., Kalden J. R. High resolution electrophoresis for the allotyping of human C4. Proposal of a relational nomenclature. J Immunol Methods. 1987 Jan 26;96(1):69–76. doi: 10.1016/0022-1759(87)90369-3. [DOI] [PubMed] [Google Scholar]
  9. Lieu T. S., Newkirk M. M., Capra J. D., Sontheimer R. D. Molecular characterization of human Ro/SS-A antigen. Amino terminal sequence of the protein moiety of human Ro/SS-A antigen and immunological activity of a corresponding synthetic peptide. J Clin Invest. 1988 Jul;82(1):96–101. doi: 10.1172/JCI113607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Malhotra R., Haurum J., Thiel S., Sim R. B. Interaction of C1q receptor with lung surfactant protein A. Eur J Immunol. 1992 Jun;22(6):1437–1445. doi: 10.1002/eji.1830220616. [DOI] [PubMed] [Google Scholar]
  11. Malhotra R., Sim R. B. Chemical and hydrodynamic characterization of the human leucocyte receptor for complement subcomponent C1q. Biochem J. 1989 Sep 1;262(2):625–631. doi: 10.1042/bj2620625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Malhotra R., Thiel S., Reid K. B., Sim R. B. Human leukocyte C1q receptor binds other soluble proteins with collagen domains. J Exp Med. 1990 Sep 1;172(3):955–959. doi: 10.1084/jem.172.3.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Malhotra V., Sim R. B. Expression of complement factor H on the cell surface of the human monocytic cell line U937. Eur J Immunol. 1985 Sep;15(9):935–941. doi: 10.1002/eji.1830150913. [DOI] [PubMed] [Google Scholar]
  14. McCauliffe D. P., Lux F. A., Lieu T. S., Sanz I., Hanke J., Newkirk M. M., Bachinski L. L., Itoh Y., Siciliano M. J., Reichlin M. Molecular cloning, expression, and chromosome 19 localization of a human Ro/SS-A autoantigen. J Clin Invest. 1990 May;85(5):1379–1391. doi: 10.1172/JCI114582. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Prahl J. W., Porter R. R. Allotype-related sequence variation of the heavy chain of rabbit immunoglobulin G. Biochem J. 1968 May;107(6):753–763. doi: 10.1042/bj1070753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rokeach L. A., Haselby J. A., Meilof J. F., Smeenk R. J., Unnasch T. R., Greene B. M., Hoch S. O. Characterization of the autoantigen calreticulin. J Immunol. 1991 Nov 1;147(9):3031–3039. [PubMed] [Google Scholar]
  17. Smith M. J., Koch G. L. Multiple zones in the sequence of calreticulin (CRP55, calregulin, HACBP), a major calcium binding ER/SR protein. EMBO J. 1989 Dec 1;8(12):3581–3586. doi: 10.1002/j.1460-2075.1989.tb08530.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tenner A. J. C1q interactions with cell surface receptors. Behring Inst Mitt. 1989 Jul;(84):220–229. [PubMed] [Google Scholar]
  19. Thiel S., Reid K. B. Structures and functions associated with the group of mammalian lectins containing collagen-like sequences. FEBS Lett. 1989 Jun 19;250(1):78–84. doi: 10.1016/0014-5793(89)80689-1. [DOI] [PubMed] [Google Scholar]
  20. Unnasch T. R., Gallin M. Y., Soboslay P. T., Erttmann K. D., Greene B. M. Isolation and characterization of expression cDNA clones encoding antigens of Onchocerca volvulus infective larvae. J Clin Invest. 1988 Jul;82(1):262–269. doi: 10.1172/JCI113581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Venables P. J., Smith P. R., Maini R. N. Purification and characterization of the Sjögren's syndrome A and B antigens. Clin Exp Immunol. 1983 Dec;54(3):731–738. [PMC free article] [PubMed] [Google Scholar]
  22. Wu D. H., Tavoni A., Garzelli C., Neri R., Vitali C., Bombardieri S. A simple method for the biochemical purification of Ro/SS-A antigen. J Immunol Methods. 1989 Jul 26;121(2):219–224. doi: 10.1016/0022-1759(89)90163-4. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES