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. 1993 Jun;79(2):196–202.

Increase of Thy-1 antigen on the thymocytes accompanied with their augmented adhesion capacity to thymic epithelial cells in the mice infected with Listeria monocytogenes.

Y Maeda 1, Y Koga 1, K Tanaka 1, X Y Zhang 1, K Nomoto 1
PMCID: PMC1421865  PMID: 8102117

Abstract

It becomes increasingly clear that adhesion systems such as CD2/LFA-3 (lymphocyte function-associated antigen-3), LFA-1/ICAM-1 (intercellular adhesion molecule-1) and Thy-1/putative Thy-1 ligand participate in the association between murine thymocytes and thymic epithelial cells. In the present study, thymocytes showed an increase in surface Thy-1 levels in mice infected with Listeria monocytogenes, but no significant changes in the levels of CD2 or LFA-1. No alteration was found either in the ratio of CD3high/CD3low/CD3- or in that of CD4/CD8 subsets in these thymocytes compared with uninfected control thymocytes which excluded the possibility of enrichment of 'cortical thymocytes' with Thy-1high/CD3low/CD4+ CD8+ in the thymocyte population of infected mice. Moreover such Thy-1high thymocytes exhibited a highly augmented ability of adhesion to a thymic epithelial cell line due to the increase of surface Thy-1 antigens as an adhesion molecule. At such intervals after infection, the total number of thymocytes was found to be reduced. These results suggest that the expression level of surface Thy-1 on thymocytes is regulated in response to in vivo stimulation and may play a role in the intrathymic development of thymocytes by affecting the adhesion of thymocytes with thymic stromal cells. The implication of the enhanced ability of adhesion in the decrease in the number of thymocytes is discussed.

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Selected References

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  1. Gunter K. C., Malek T. R., Shevach E. M. T cell-activating properties of an anti-Thy-1 monoclonal antibody. Possible analogy to OKT3/Leu-4. J Exp Med. 1984 Mar 1;159(3):716–730. doi: 10.1084/jem.159.3.716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. He H. T., Naquet P., Caillol D., Pierres M. Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca2(+)-independent mechanism. J Exp Med. 1991 Feb 1;173(2):515–518. doi: 10.1084/jem.173.2.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hoessli D., Bron C., Pink J. R. T-lymphocyte differentiation is accompanied by increase in sialic acid content of Thy-1 antigen. Nature. 1980 Feb 7;283(5747):576–578. doi: 10.1038/283576a0. [DOI] [PubMed] [Google Scholar]
  4. Killar L. M., Eisenstein T. K. Delayed-type hypersensitivity and immunity to Salmonella typhimurium. Infect Immun. 1986 May;52(2):504–508. doi: 10.1128/iai.52.2.504-508.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Koga Y., Tanaka K., Yokoyama M., Taniguchi K., Nomoto K. Thymus cell migration in a prostaglandin-mediated system. Immunobiology. 1985 Jul;169(5):486–502. doi: 10.1016/S0171-2985(85)80004-8. [DOI] [PubMed] [Google Scholar]
  6. Koga Y., Taniguchi K., Nomoto K. T-cell recruitment regulated by prostaglandin-mediated system and its role in immune response. Immunobiology. 1984 May;166(4-5):382–396. doi: 10.1016/s0171-2985(84)80016-9. [DOI] [PubMed] [Google Scholar]
  7. Kroczek R. A., Gunter K. C., Germain R. N., Shevach E. M. Thy-1 functions as a signal transduction molecule in T lymphocytes and transfected B lymphocytes. Nature. 1986 Jul 10;322(6075):181–184. doi: 10.1038/322181a0. [DOI] [PubMed] [Google Scholar]
  8. Lane F. C., Unanue E. R. Requirement of thymus (T) lymphocytes for resistance to listeriosis. J Exp Med. 1972 May 1;135(5):1104–1112. doi: 10.1084/jem.135.5.1104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lepesant H., Reggio H., Pierres M., Naquet P. Mouse thymic epithelial cell lines interact with and select a CD3lowCD4+CD8+ thymocyte subset through an LFA-1-dependent adhesion--de-adhesion mechanism. Int Immunol. 1990;2(11):1021–1032. doi: 10.1093/intimm/2.11.1021. [DOI] [PubMed] [Google Scholar]
  10. Maeda Y., Tanaka K., Koga Y., Zhang X. Y., Sasaki M., Kimura G., Nomoto K. A simple quantitative in vitro assay for thymocyte adhesion to thymic epithelial cells using a fluorescein diacetate. J Immunol Methods. 1993 Jan 4;157(1-2):117–123. doi: 10.1016/0022-1759(93)90077-k. [DOI] [PubMed] [Google Scholar]
  11. McClay D. R., Wessel G. M., Marchase R. B. Intercellular recognition: quantitation of initial binding events. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4975–4979. doi: 10.1073/pnas.78.8.4975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mitsuyama M., Takeya K., Nomoto K., Shimotori S. Three phases of phagocyte contribution to resistance against Listeria monocytogenes. J Gen Microbiol. 1978 May;106(1):165–171. doi: 10.1099/00221287-106-1-165. [DOI] [PubMed] [Google Scholar]
  13. Moroi Y., Koga Y., Nakamura K., Ohtsu M., Kimura G., Nomoto K. Accumulation of p60lck in HTLV-I-transformed T cell lines detected by an anti-lck monoclonal antibody, MOL 171. Jpn J Cancer Res. 1991 Aug;82(8):909–915. doi: 10.1111/j.1349-7006.1991.tb01920.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nakashima M., Mori K., Maeda K., Kishi H., Hirata K., Kawabuchi M., Watanabe T. Selective elimination of double-positive immature thymocytes by a thymic epithelial cell line. Eur J Immunol. 1990 Jan;20(1):47–53. doi: 10.1002/eji.1830200108. [DOI] [PubMed] [Google Scholar]
  15. Nomoto K., Shimamoto Y., Taniguchi K., Kubo C., Kawauchi H., Mitsuyama M., Takeya K. Development of immunity against Listeria monocytogenes in athymic nude versus neonatally thymectomized mice. Cell Immunol. 1983 Jan;75(1):134–143. doi: 10.1016/0008-8749(83)90312-x. [DOI] [PubMed] [Google Scholar]
  16. Orme I. M., Collins F. M. Protection against Mycobacterium tuberculosis infection by adoptive immunotherapy. Requirement for T cell-deficient recipients. J Exp Med. 1983 Jul 1;158(1):74–83. doi: 10.1084/jem.158.1.74. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Papiernik M., Penit C., el Rouby S. Control of prothymocyte proliferation by thymic accessory cells. Eur J Immunol. 1987 Sep;17(9):1303–1310. doi: 10.1002/eji.1830170913. [DOI] [PubMed] [Google Scholar]
  18. Rotman B., Papermaster B. W. Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters. Proc Natl Acad Sci U S A. 1966 Jan;55(1):134–141. doi: 10.1073/pnas.55.1.134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Singer K. H., Denning S. M., Whichard L. P., Haynes B. F. Thymocyte LFA-1 and thymic epithelial cell ICAM-1 molecules mediate binding of activated human thymocytes to thymic epithelial cells. J Immunol. 1990 Apr 15;144(8):2931–2939. [PubMed] [Google Scholar]
  20. Sprent J., Lo D., Gao E. K., Ron Y. T cell selection in the thymus. Immunol Rev. 1988 Jan;101:173–190. doi: 10.1111/j.1600-065x.1988.tb00737.x. [DOI] [PubMed] [Google Scholar]
  21. Stutman O. Intrathymic and extrathymic T cell maturation. Immunol Rev. 1978;42:138–184. doi: 10.1111/j.1600-065x.1978.tb00261.x. [DOI] [PubMed] [Google Scholar]
  22. Tanaka K., Koga Y., Taniguchi K., Kamikaseda K., Nomoto K. T cell recruitment from the thymus to the spleen in tumor-bearing mice. I. Analysis of recruited cells by surface markers. Cancer Immunol Immunother. 1986;22(1):37–42. doi: 10.1007/BF00205714. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tanaka K., Koga Y., Taniguchi K., Nomoto K. T-cell recruitment from the thymus to the spleen in tumor bearing mice: phenotypical alteration and recruitment of thymocytes raised in a tumor bearing state. Cancer Res. 1987 Apr 15;47(8):2136–2141. [PubMed] [Google Scholar]
  24. Vollger L. W., Tuck D. T., Springer T. A., Haynes B. F., Singer K. H. Thymocyte binding to human thymic epithelial cells is inhibited by monoclonal antibodies to CD-2 and LFA-3 antigens. J Immunol. 1987 Jan 15;138(2):358–363. [PubMed] [Google Scholar]
  25. Weissman I. L. Thymus cell maturation. Studies on the origin of cortisone-resistant thymic lymphocytes. J Exp Med. 1973 Feb 1;137(2):504–510. doi: 10.1084/jem.137.2.504. [DOI] [PMC free article] [PubMed] [Google Scholar]

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