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. 1993 Jul;79(3):362–367.

Human T-helper cell responses to a synthetic peptide derived from the hepatitis B surface antigen.

A Mishra 1, K V Rao 1, H Durgapal 1, V Manivel 1, S K Panda 1
PMCID: PMC1421980  PMID: 7691722

Abstract

Hepatitis B virus surface antigen peptide OS (aa124-147) self oligomerizes to form conformational B-cell immunogen with several properties of a candidate peptide vaccine. It gives a T-cell blastogenic response in vaccinated as well as naturally infected individuals. To study the nature and localization of the T-helper cell epitopes, the T-lymphocyte proliferative responses in humans exposed to hepatitis B surface antigen (HBsAg) were examined with a synthetic peptide representing residues 124-147 of this antigen [peptide OS (aa24-147)]. Positive responses were obtained in most cases regardless of whether HBsAg exposure was due to vaccination or a hepatitis B viral infection. Epitope localization studies with truncated peptides indicated the presence of more than two HBsAg-relevant T-helper cell epitopes. This was also corroborated by our fine mapping studies which revealed that the amino acid residues crucial for eventual T-helper cell activation were diverse amongst the various individuals. Together these studies suggest that immunization with peptide OS (aa124-147) may result in an HBsAg cross-reactive T-helper cell response in a broad spectrum of the human population.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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