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. 1973 Aug;25(2):167–177.

Haemolysis in chicken serum

I. The ionic environment

Ann E Gabrielsen, R J Pickering, R A Good
PMCID: PMC1422845  PMID: 4739067

Abstract

Immune haemolysis in chicken serum was examined using rabbit cells, sensitized with chicken antibody. Salt concentration was a major influence on complement activity: titres were greatly increased at ionic strengths which sustain negligible activity of human, rabbit, or guinea-pig complements. Sensitivity of the assay was also increased if standard Mg+ + was lowered to 7.5 × 10-4 M and Ca+ + increased to 6–9 × 10-4 M. Cobalt ion and Ba+ + facilitated complement activity; Ni+ + did not. Cobalt ion seemed to act as an Mg+ + substitute, and barium as a replacement for Ca+ +. The kinetics of haemolysis in this system resembled those of human complement: a rapid initial rate, followed by a slower one, but no levelling off at 4 hours.

Natural antibody to rabbit cells may be excluded from the titration by absorption or by limiting Mg+ + in the buffer. The nature of this Mg+ + dependence is unknown.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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