Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1973 Jan;24(1):177–189.

The interaction of normal lymphocytes and cells from lymphoid cell lines

III. Studies on activation in an autochthonous system

C M Steel, D A Hardy, N R Ling, H M Dick, P Mackintosh, W B Crichton
PMCID: PMC1422866  PMID: 4119542

Abstract

Activation of fresh blood lymphocytes has been measured by uptake of tritiated thymidine, following exposure to irradiated cells from autochthonous or allogeneic cell lines (LCL cells). Nine autochthonous combinations were studied. In every case activation was observed and in one the rate and peak level of activation were comparable to those found in control allogeneic mixtures. Neither foetal calf serum nor antibiotics appeared to be important factors in the activation process. There was no correlation between the number of identified HL-A histoincompatibilities in a given allogeneic mixture and the rate or peak level of activation achieved. Activation appears to be influenced by the metabolic state of the LCL cells and the rate of the reaction is sensitive to the precise culture conditions under which it proceeds. Some distinction can be drawn between the antigens responsible for activation in autochthonous and in allogeneic mixtures but the former may prove to be modified histocompatibility determinants.

Full text

PDF
177

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amos D. B., Bach F. H. Phenotypic expressions of the major histocompatibility locus in man (HL-A): leukocyte antigens and mixed leukocyte culture reactivity. J Exp Med. 1968 Oct 1;128(4):623–637. doi: 10.1084/jem.128.4.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bernoco D., Glade P. R., Broder S., Miggiano V. C., Hirschhorn K., Ceppellini R. Stability of HL-A and appearance of other antigens (LIVA) at the surface of lymphoblasts grown in vitro. Haematologica. 1969;54(11):795–812. [PubMed] [Google Scholar]
  3. Burnet F. M. A certain symmetry: histocompatibility antigens compared with immunocyte receptors. Nature. 1970 Apr 11;226(5241):123–126. doi: 10.1038/226123a0. [DOI] [PubMed] [Google Scholar]
  4. Flier J. S., Glade P. R., Broder S. W., Hirschhorn K. Lymphocyte stimulation by allogeneic and autochthonous cultured lymphoid cells. Cell Immunol. 1970 Dec;1(6):596–602. doi: 10.1016/0008-8749(70)90025-0. [DOI] [PubMed] [Google Scholar]
  5. Green S. S., Sell K. W. Mixed leukocyte stimulation of normal peripheral leukocytes by autologous lymphoblastoid cells. Science. 1970 Nov 27;170(3961):989–990. doi: 10.1126/science.170.3961.989. [DOI] [PubMed] [Google Scholar]
  6. HAMBURGER R. N., PIOUS D. A., MILLS S. E. ANTIGENIC SPECIFICITIES ACQUIRED FROM THE GROWTH MEDIUM BY CELLS IN TISSUE CULTURE. Immunology. 1963 Sep;6:439–449. [PMC free article] [PubMed] [Google Scholar]
  7. Habel K. Antigens of virus-induced tumors. Adv Immunol. 1969;10:229–250. doi: 10.1016/s0065-2776(08)60418-6. [DOI] [PubMed] [Google Scholar]
  8. Han T., Moore G. E., Sokal J. E. In vitro lymphocyte response to autologous cultured lymphoid cells. Proc Soc Exp Biol Med. 1971 Mar;136(3):976–979. doi: 10.3181/00379727-136-35409. [DOI] [PubMed] [Google Scholar]
  9. Hardy D. A., Knight S., Ling N. R. The interaction of normal lymphocytes and cells from lymphoid cell lines. I. The nature of the activation process. Immunology. 1970 Aug;19(2):329–342. [PMC free article] [PubMed] [Google Scholar]
  10. Hardy D. A., Ling N. R. Effects of some cellular antigens on lymphocytes and the nature of the mixed lymphocyte reaction. Nature. 1969 Feb 8;221(5180):545–548. doi: 10.1038/221545a0. [DOI] [PubMed] [Google Scholar]
  11. Hardy D. A., Ling N. R., Knight S. C. Exceptional lymphocyte stimulating capacity of cells from lymphoid cell lines. Nature. 1969 Aug 2;223(5205):511–512. doi: 10.1038/223511a0. [DOI] [PubMed] [Google Scholar]
  12. Hardy D. A., Ling N. R., Wallin J., Aviet T. Destruction of lymphoid cells by activated human lymphocytes. Nature. 1970 Aug 15;227(5259):723–725. doi: 10.1038/227723a0. [DOI] [PubMed] [Google Scholar]
  13. Hardy D. A., Steel C. M. Cytotoxic potential of lymphocytes stimulated with autochthonous lymphoid cell line cells. Experientia. 1971;27(11):1336–1338. doi: 10.1007/BF02136724. [DOI] [PubMed] [Google Scholar]
  14. Johnston J. M., Bashir H. V. Response of HL-A identical unrelated individuals in the mixed lymphocyte culture test. Br Med J. 1971 Dec 4;4(5787):581–584. doi: 10.1136/bmj.4.5787.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Junge U., Hoekstra J., Deinhardt F. Altered antigenicity of cultured mononucleosis lymphocytes. Lancet. 1970 Jul 25;2(7665):217–217. doi: 10.1016/s0140-6736(70)92574-2. [DOI] [PubMed] [Google Scholar]
  16. Junge U., Hoekstra J., Deinhardt F. Stimulation of peripheral lymphocytes by allogeneic and autochthonous mononucleosis lymphocyte cell lines. J Immunol. 1971 May;106(5):1306–1315. [PubMed] [Google Scholar]
  17. Kissmeyer-Nielsen F., Svejgaard A., Sorensen S. F., Nielsen L. S., Thorsby E. Mixed lymphocyte cultures and HL-A identity in unrelated subjects. Nature. 1970 Oct 3;228(5266):63–65. doi: 10.1038/228063a0. [DOI] [PubMed] [Google Scholar]
  18. Knight S. C., Moore G. E., Clarkson B. D. Stimulation of autochthonous lymphocytes by cells from normal and leukaemic lines. Nat New Biol. 1971 Feb 10;229(6):185–187. doi: 10.1038/newbio229185a0. [DOI] [PubMed] [Google Scholar]
  19. Knight S., Hardy D. A., Ling N. R. The interaction of normal lymphocytes and cells from lymphoid cell lines. II. Variations relating to cell line source and lymphocyte donor. Immunology. 1970 Aug;19(2):343–351. [PMC free article] [PubMed] [Google Scholar]
  20. Rogentine G. N., Jr, Gerber P. HL-A antigens of human lymphoid cells in long-term tissue culture. Transplantation. 1969 Jul;8(1):28–37. doi: 10.1097/00007890-196907000-00004. [DOI] [PubMed] [Google Scholar]
  21. Schellekens P. T., Vriesendorp B., Eijsvoogel V. P., Van Leeuwen A., Van Rood J. J., Miggiano V., Ceppellini R. Lymphocyte transformation in vitro. II. Mixed lymphocyte culture in relation to leucocyte antigens. Clin Exp Immunol. 1970 Feb;6(2):241–254. [PMC free article] [PubMed] [Google Scholar]
  22. Steel C. M., Edmond E. Human lymphoblastoid cell lines. I. Culture methods and examination for Epstein-Barr virus. J Natl Cancer Inst. 1971 Dec;47(6):1193–1201. [PubMed] [Google Scholar]
  23. Steel C. M., Hardy D. A. Evidence of altered antigenicity in cultured lymphoid cells from patients with infectious mononucleosis. Lancet. 1970 Jun 20;760(1):1322–1323. doi: 10.1016/s0140-6736(70)91913-6. [DOI] [PubMed] [Google Scholar]
  24. Steel C. M. Human lymphoblastoid cell lines. 3. Co-cultivation technique for establishment of new lines. J Natl Cancer Inst. 1972 Mar;48(3):623–628. [PubMed] [Google Scholar]
  25. van Rood J. J., Eijsvoogel V. P. HL-A identical phenotypes and genotypes in unrelated individuals. Lancet. 1970 Apr 4;1(7649):698–700. doi: 10.1016/s0140-6736(70)90927-x. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES