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. 1973 Apr;24(4):759–770.

Developed γM haemolytic plaque-forming cells in chickens

F L Shand, J Ivanyi
PMCID: PMC1422907  PMID: 4574801

Abstract

Incorporation of rabbit anti-μ serum into agarose gels inhibited direct anti-SRBC PFCs using chicken complement, but developed equal numbers of indirect PFCs binding guinea-pig complement. The majority of attempts to demonstrate direct PFCs against HSA were unsuccessful, although low numbers of barely discernible PFCs were obtained by using HSA-SRBC sensitized with carbodiimide at room temperature; indirect PFCs were developed, however, by the anti-μ serum. During the primary response, developed anti-HSA PFCs could not be assigned to IgM or IgG classes using reduction and alkylation. Concanavalin A completely inhibited direct anti-SRBC and SIII PFCs, although developed γM PFCs were only inhibited partially. The identity of direct and anti-μ developed PFCs against SIII was demonstrated by the replica technique. None of the approaches applied provided any support for the existence of structural heterogeneity of IgM within the PFC population.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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