Abstract
A method for the quantitation of cytotoxic action of antiserum and complement is described, measuring the incorporation of trypan blue into Ehrlich ascites tumour cells.
The incorporation of the dye into the cells in the presence of antiserum and complement was maximum at ionic strength between 0.10 and 0.15, and decreased with increasing ionic strength higher than 0.15. The permeability barrier of the cell against the dye was stable at pH between 7.2 and 7.4. The cytotoxic reaction required Mg++ and Ca++ ion, but was inhibited with concentrations higher than 5 mM Mg++ and 1 MNCa. The incorporation of the dye in the presence of antiserum and complement was more rapid with increasing concentrations of the dye, and depended on temperature.
From these findings we obtained optimal condition for the assay of cytotoxic antiserum.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BECKER E. L. Concerning the mechanism of complement action. IV. The properties of activated first component of guinea pig complement. J Immunol. 1959 Jan;82(1):43–51. [PubMed] [Google Scholar]
- REIF A. E. Immune cytolysis of three mouse ascites tumors. J Immunol. 1962 Dec;89:849–860. [PubMed] [Google Scholar]