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. 1973 Apr;24(4):601–606.

Separation and characterization of cell subpopulations in the thymus

D Sabolović, F Dumont
PMCID: PMC1422914  PMID: 4574795

Abstract

Electrophoretic mobility and PHA responsiveness were studied in mouse thymus cells fractionated on albumin gradient. It was demonstrated that cells with the same electrophoretic mobility may have different buoyant densities.

Cortisone-sensitive cells were found to have slow electrophoretic mobility whereas cortisone-resistant PHA responsive cells had fast electrophoretic mobility. Both populations were recovered throughout the gradient but in different proportions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson B., Blomgren H. Evidence for a small pool of immunocompetent cells in the mouse thymus. Its role in the humoral antibody response against sheep erythrocytes, bovine serum albumin, ovalbumin and the NIP determinant. Cell Immunol. 1970 Oct;1(4):362–371. doi: 10.1016/0008-8749(70)90014-6. [DOI] [PubMed] [Google Scholar]
  2. BANGHAM A. D., HEARD D. H., FLEMANS R., SEAMAN G. V. An apparatus for microelectrophoresis of small particles. Nature. 1958 Sep 6;182(4636):642–644. doi: 10.1038/182642a0. [DOI] [PubMed] [Google Scholar]
  3. Blomgren H., Andersson B. Characteristics of the immunocompetent cells in the mouse thymus: cell population changes during cortisone-induced atrophy and subsequent regeneration. Cell Immunol. 1970 Nov;1(5):545–560. doi: 10.1016/0008-8749(70)90041-9. [DOI] [PubMed] [Google Scholar]
  4. Blomgren H., Andersson B. Evidence for a small pool of immunocompetent cells in the mouse thymus. Exp Cell Res. 1969 Oct;57(2):185–192. doi: 10.1016/0014-4827(69)90140-2. [DOI] [PubMed] [Google Scholar]
  5. Blomgren H., Svedmyr E. In vitro stimulation of mouse thymus cells by PHA and allogeneic cells. Cell Immunol. 1971 Aug;2(4):285–299. doi: 10.1016/0008-8749(71)90063-3. [DOI] [PubMed] [Google Scholar]
  6. Cohen J. J., Fschbach M., Claman H. N. Hydrocortisne resistance of graft vs host activity in mouse thymus, spleen and bone marrow. J Immunol. 1970 Nov;105(5):1146–1150. [PubMed] [Google Scholar]
  7. Colley D. G., Wu A. Y., Waksman B. H. Cellular differentiation in the thymus. 3. Surface properties of rat thymus and lymph node cells separated on density gradients. J Exp Med. 1970 Dec 1;132(6):1107–1121. doi: 10.1084/jem.132.6.1107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lance E. M., Cooper S., Boyse E. A. Antigenic change and cell maturation in murine thymocytes. Cell Immunol. 1970 Nov;1(5):536–544. doi: 10.1016/0008-8749(70)90040-7. [DOI] [PubMed] [Google Scholar]
  9. Leckband E., Boyse E. A. Immunocompetent cells among mouse thymocytes: a minor population. Science. 1971 Jun 18;172(3989):1258–1260. doi: 10.1126/science.172.3989.1258. [DOI] [PubMed] [Google Scholar]
  10. Raff M. Evidence for subpopulation of mature lymphocytes within mouse thymus. Nat New Biol. 1971 Feb 10;229(6):182–184. doi: 10.1038/newbio229182a0. [DOI] [PubMed] [Google Scholar]
  11. Ruhenstroth-Bauer G., Lücke-Huhle C. Two populations of small lymphocytes. J Cell Biol. 1968 Apr;37(1):196–199. doi: 10.1083/jcb.37.1.196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schlesinger M., Golakai V. K. Loss of thymus-distinctive serological characteristics in mice under certain conditions. Science. 1967 Mar 3;155(3766):1114–1116. doi: 10.1126/science.155.3766.1114. [DOI] [PubMed] [Google Scholar]
  13. Shortman K., Brunner K. T., Cerottini J. C. Separation of stages in the development of the "T" cells involved in cell-mediated immunity. J Exp Med. 1972 Jun 1;135(6):1375–1391. doi: 10.1084/jem.135.6.1375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shortman K., Cerottini J. C., Brunner K. T. The separation of sub-populations of T and B lymphocytes. Eur J Immunol. 1972 Aug;2(4):313–319. doi: 10.1002/eji.1830020404. [DOI] [PubMed] [Google Scholar]
  15. Wioland M., Sabolovic D., Burg C. Electrophoretic mobilities of T and B cells. Nat New Biol. 1972 Jun 28;237(78):274–276. doi: 10.1038/newbio237274a0. [DOI] [PubMed] [Google Scholar]

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