Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1973 Jun;24(6):939–954.

Antigenic characterization of murine rosette and plaque-forming cells

R Laskov, R Rabinowitz, M Schlesinger
PMCID: PMC1422940  PMID: 4123963

Abstract

Specific antisera reacting with mouse B and T lymphocytes were raised by immunizing rabbits with: (1) mouse myeloma tumour cells; (2) bone-marrow cells; (3) thymus cells, and proper absorptions of the antisera obtained. These antisera together with anti-IgG and anti-θ were used to characterize surface antigens on antibody-producing cells. It was found that anti-bone marrow serum, absorbed with thymus cells in the presence of guinea-pig C, completely inhibited in vitro the action of all types of antibody-producing cells, i.e. direct and indirect PFC, and normal and immune-RFC. Anti-myeloma serum absorbed with thymus cells inhibited direct and indirect PFC, most of the immune-RFC, but did not inhibit normal RFC. Absorption with both thymus and bone-marrow cells rendered the anti-myeloma serum specific for an antigen present on PFC and myeloma cells (My antigen). Anti-IgG completely inhibited both normal and immune RFC but had only a minor inhibitory effect on PFC. On the other hand, antithymus absorbed with bone marrow cells, like anti-θ, did not inhibit PFC, and occasionally inhibited a small percentage of RFC. With the aid of these heterologous antisera at least three antigenic determinants could be defined on the surface of antibody-producing B lymphocytes: B1, B2 and My. Thus, normal-RFC were shown to be antigenically different from immune-RFC and both lack an antigen present in PFC. In addition, all these cells contained the B1 antigen, which was partially or completely missing from myeloma tumour cells.

Full text

PDF
939

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ada G. L. Antigen binding cells in tolerance and immunity. Transplant Rev. 1970;5:105–129. doi: 10.1111/j.1600-065x.1970.tb00358.x. [DOI] [PubMed] [Google Scholar]
  2. Bach J. F., Muller J. Y., Dardenne M. In vivo specific antigen recognition by rosette forming cells. Nature. 1970 Sep 19;227(5264):1251–1252. doi: 10.1038/2271251a0. [DOI] [PubMed] [Google Scholar]
  3. Baird S., Santa J., Weissman I. Anti-theta antisera may contain anti-allotype contamination. Nat New Biol. 1971 Jul 14;232(28):56–56. doi: 10.1038/newbio232056a0. [DOI] [PubMed] [Google Scholar]
  4. Cohen A., Schlesinger M. Absorption of guinea pig serum with agar. A method for elimination of itscytotoxicity for murine thymus cells. Transplantation. 1970 Jul;10(1):130–132. doi: 10.1097/00007890-197007000-00027. [DOI] [PubMed] [Google Scholar]
  5. Cunningham A. J., Smith J. B., Mercer E. H. Antibody formation by single cells from lymph nodes and efferent lymph of sheep. J Exp Med. 1966 Oct 1;124(4):701–714. doi: 10.1084/jem.124.4.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dresser D. W., Wortis D. H. Use of an antiglobulin serum to detect cells producing antibody with low haemolytic efficiency. Nature. 1965 Nov 27;208(5013):859–861. doi: 10.1038/208859a0. [DOI] [PubMed] [Google Scholar]
  7. Golub E. S. Brain-associated theta antigen: reactivity of rabbit anti-mouse brain with mouse lymphoid cells. Cell Immunol. 1971 Aug;2(4):353–361. doi: 10.1016/0008-8749(71)90070-0. [DOI] [PubMed] [Google Scholar]
  8. Greaves M. F., Möller E. Studies on antigen-binding cells. I. The origin of reactive cells. Cell Immunol. 1970 Oct;1(4):372–385. doi: 10.1016/0008-8749(70)90015-8. [DOI] [PubMed] [Google Scholar]
  9. HATTLER B. G., Jr, SCHLESINGER M., AMOS D. B. THE DIFFERING SURVIVAL OF NORMAL AND SENSITIZED SPLEEN CELLS TRANSFERRED TO ALLOGENEIC HOSTS. J Exp Med. 1964 Nov 1;120:783–793. doi: 10.1084/jem.120.5.783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Laskov R. Rosette forming cells in non-immunized mice. Nature. 1968 Aug 31;219(5157):973–975. doi: 10.1038/219973a0. [DOI] [PubMed] [Google Scholar]
  11. Laskov R., Scharff M. D. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits. J Exp Med. 1970 Mar 1;131(3):515–541. doi: 10.1084/jem.131.3.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McConnell I. Antigen receptors on the surface of antibody-secreting cells. Nat New Biol. 1971 Oct 6;233(40):177–179. doi: 10.1038/newbio233177a0. [DOI] [PubMed] [Google Scholar]
  13. Miller J. F., Basten A., Sprent J., Cheers C. Interaction between lymphocytes in immune responses. Cell Immunol. 1971 Oct;2(5):469–495. doi: 10.1016/0008-8749(71)90057-8. [DOI] [PubMed] [Google Scholar]
  14. Moav N., Harris T. N. Rosette formation in relation to active synthesis of antibody. J Immunol. 1970 Dec;105(6):1501–1511. [PubMed] [Google Scholar]
  15. Osoba D. Some physical and radiobiological properties of immunologically reactive mouse spleen cells. J Exp Med. 1970 Aug 1;132(2):368–383. doi: 10.1084/jem.132.2.368. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Paraskevas F., Lee S. T., Israels L. G. Absence of gamma-globulin receptors on mouse plasmacytoma cells. Nature. 1970 Jul 25;227(5256):395–397. doi: 10.1038/227395a0. [DOI] [PubMed] [Google Scholar]
  17. Playfair J. H., Purves E. C. Separate thymus dependent and thymus independent antibody forming cell precursors. Nat New Biol. 1971 Jun 2;231(22):149–151. doi: 10.1038/newbio231149a0. [DOI] [PubMed] [Google Scholar]
  18. REIF A. E., ALLEN J. M. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med. 1964 Sep 1;120:413–433. doi: 10.1084/jem.120.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Raff M. C. Surface antigenic markers for distinguishing T and B lymphocytes in mice. Transplant Rev. 1971;6:52–80. doi: 10.1111/j.1600-065x.1971.tb00459.x. [DOI] [PubMed] [Google Scholar]
  20. Raff M. Theta isoantigen as a marker of thymus-derived lymphocytes in mice. Nature. 1969 Oct 25;224(5217):378–379. doi: 10.1038/224378a0. [DOI] [PubMed] [Google Scholar]
  21. Schlesinger M. Anti-theta antibodies for detecting thymus-dependent lymphocytes in the immune response of mice to SRBC. Nature. 1970 Jun 27;226(5252):1254–1256. doi: 10.1038/2261254a0. [DOI] [PubMed] [Google Scholar]
  22. Schlesinger M., Cohen A., Hurvitz D. Characterization of cytotoxic isoantisera produced in RIII mice. II. Serological properties of antiserum fractions. Isr J Med Sci. 1969 Mar-Apr;5(2):235–244. [PubMed] [Google Scholar]
  23. Segal S., Cohen I. R., Feldman M. Thymus-derived lymphocytes: humoral and cellular reactions distinguished by hydrocortisone. Science. 1972 Mar 10;175(4026):1126–1128. doi: 10.1126/science.175.4026.1126. [DOI] [PubMed] [Google Scholar]
  24. Shigeno N., Hämmerling U., Arpels C., Boyse E. A., Old L. J. Preparation of lymphocyte-specific antibody from anti-lymphocyte serum. Lancet. 1968 Aug 10;2(7563):320–323. doi: 10.1016/s0140-6736(68)90530-8. [DOI] [PubMed] [Google Scholar]
  25. Takahashi T., Carswell E. A., Thorbecke G. J. Surface antigens of immunocompetent cells. I. Effect of theta and PC.1 alloantisera on the ability of spleen cells to transfer immune responses. J Exp Med. 1970 Dec 1;132(6):1181–1190. doi: 10.1084/jem.132.6.1181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Takahashi T., Old L. J., Boyse E. A. Surface alloantigens of plasma cells. J Exp Med. 1970 Jun 1;131(6):1325–1341. doi: 10.1084/jem.131.6.1325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Takahashi T., Old L. J., McIntire K. R., Boyse E. A. Immunoglobulin and other surface antigens of cells of the immune system. J Exp Med. 1971 Oct 1;134(4):815–832. doi: 10.1084/jem.134.4.815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Watanabe T., Yagi Y., Pressman D. Antibody against neoplastic plasma cells. I. Specific surface antigens on mouse myeloma cells. J Immunol. 1971 May;106(5):1213–1221. [PubMed] [Google Scholar]
  29. Wigzell H. Specific fractionation of immunocompetent cells. Transplant Rev. 1970;5:76–104. doi: 10.1111/j.1600-065x.1970.tb00357.x. [DOI] [PubMed] [Google Scholar]
  30. Wilson J. D., Miller J. F. T and B rosette-forming cells. Eur J Immunol. 1971 Dec;1(6):501–503. doi: 10.1002/eji.1830010622. [DOI] [PubMed] [Google Scholar]
  31. Zaalberg O. B., van der Meul V. A., van Twisk M. J. Antibody production by isolated spleen cells: a study of the cluster and the plaque techniques. J Immunol. 1968 Feb;100(2):451–458. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES