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. 1973 Mar;24(3):445–453.

A physicochemical approach to the characterization of red cell antigen—antibody systems*

B J Oberhardt, P Lalezari, A F Jiang
PMCID: PMC1422967  PMID: 4705615

Abstract

Physicochemical parameters of antigen—antibody reactions in various blood group systems were studied by means of a modified Polybrene technique, performed in a continuous flow system. Antibody-dependent aggregates were deaggregated by exposure to progressively increasing temperature. The temperature at which one-half of the aggregates dissociate (T 50 per cent) has been found to depend upon thermal characteristics as well as the concentration of the antibodies involved. In accordance with the results of a kinetic study of this deaggregation phenomenon, a plot of the logarithm of the antibody concentration versus the reciprocal of T 50 per cent (in absolute temperature units) was found to produce a straight line. Such plots were obtained for different examples of anti-Rh0, -M, -I, and single examples of anti-rh′, -hr′, -A, -S, -Fya, -Jka and -Kell antibodies. The results indicate that each system possesses a characteristic slope, and intercept with a position in a predictable thermal area. The slopes were interpreted in terms of the activation energy required for breaking the antigen—antibody bonds. This approach provides a means by which thermal characteristics of antigen—antibody systems can be determined independently of antibody concentration. The approach allows the differentiation of serologically distinct antigen—antibody systems based upon information extracted from the dissociation behaviour of aggregated red cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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