Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1973 Mar;24(3):467–475.

Expulsion of Nippostrongylus brasiliensis from the intestine of rats

Collaboration between humoral and cellular components of the immune response

J K Dineen, Bridget M Ogilvie, J D Kelly
PMCID: PMC1422980  PMID: 4705616

Abstract

The role played by immune lymph node cells in expulsion of normal, damaged and adapted adult worms (Ogilvie and Hockley, 1968) from the small intestine of the rat was studied. Sensitized mesenteric lymph node cells caused the rapid and complete expulsion of worms which had previously been damaged by the action of antibodies. Over the same time, about 50 per cent of normal worms were expelled from cell recipients, and there was a ten-fold reduction in egg production by these worms. Adapted worms were markedly less susceptible than normal worms to the immunological attack mounted by the transferred cells.

These results confirm and extend those of Jones and Ogilvie (1971) and Keller and Keist (1972) and show that worm expulsion occurs in two steps (1) antibody action followed by (2) cellular action and that cells are not effective until step (1) has occurred. It seems that worm expulsion is caused by a rate limiting factor which is triggered by interaction of antigen with sensitized lymphocytes. These results are related to previous studies of immunity to this and other nematodes.

Full text

PDF
467

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AGLAND B. M., DINEEN J. K. THE CELLULAR TRANSFER OF IMMUNITY TO TRICHOSTRONGYLUS COLUBRIFORMIS IN AN ISOGENIC STRAIN OF GUINES-PIG. Aust J Exp Biol Med Sci. 1965 Jul;43:429–438. doi: 10.1038/icb.1965.77. [DOI] [PubMed] [Google Scholar]
  2. Dineen J. K., Adams D. B. The role of the recirculating thymus-dependent lymphocyte in resistance to Trichostrongylus colubriformis in the guinea-pig. Immunology. 1971 Jan;20(1):109–113. [PMC free article] [PubMed] [Google Scholar]
  3. Dineen J. K., Wagland B. M. The cellular transfer of immunity to Trichostrongylus colubriformis in an isogenic strain of guinea-pig. II. The relative susceptibility of the larval and adult stages of the parasite to immunological attack. Immunology. 1966 Jul;11(1):47–57. [PMC free article] [PubMed] [Google Scholar]
  4. Dumonde D. C., Wolstencroft R. A., Panayi G. S., Matthew M., Morley J., Howson W. T. "Lymphokines": non-antibody mediators of cellular immunity generated by lymphocyte activation. Nature. 1969 Oct 4;224(5214):38–42. doi: 10.1038/224038a0. [DOI] [PubMed] [Google Scholar]
  5. HUNTER G. C., LEIGH L. C. Studies on the resistance of rats to the nematode Nippostrongylus muris (Yokogawa, 1920). III. Attempt to transfer immunity adoptively. Parasitology. 1961 Nov;51:357–366. doi: 10.1017/s0031182000070621. [DOI] [PubMed] [Google Scholar]
  6. Jones V. E., Ogilvie B. M. Protective immunity to Nippostrongylus brasiliensis: the sequence of events which expels worms from the rat intestine. Immunology. 1971 Apr;20(4):549–561. [PMC free article] [PubMed] [Google Scholar]
  7. Kassai T., Szepes G. Experiments on adoptive transfer of immunological tolerance and of immunity to Nippostrongylus brasiliensis in rats. Acta Vet Acad Sci Hung. 1970;20(2):207–217. [PubMed] [Google Scholar]
  8. Keller R., Keist R. Protective immunity to Nippostrongylus brasiliensis in the rat. Central role of the lymphocyte in worm expulsion. Immunology. 1972 May;22(5):767–773. [PMC free article] [PubMed] [Google Scholar]
  9. Keller R. Nippostrongylus brasiliensis in the rat: failure to relate intestinal histamine and mast cell levels with worm expulsion. Parasitology. 1971 Dec;63(3):473–481. doi: 10.1017/s0031182000079993. [DOI] [PubMed] [Google Scholar]
  10. Keller R., Ogilvie B. M. The effects of drugs on worm expulsion in the Nippostrongylus brasiliensis infected rat: a discussion of the interpretation of drug action. Parasitology. 1972 Apr;64(2):217–227. doi: 10.1017/s0031182000029644. [DOI] [PubMed] [Google Scholar]
  11. Kelly J. D., Dineen J. K. The cellular transfer to immunity to Nippostrongylus brasiliensis in inbred rats (Lewis strain). Immunology. 1972 Feb;22(2):199–210. [PMC free article] [PubMed] [Google Scholar]
  12. Kelly J. D., Ogilvie B. M. Intestinal mast cell and eosinophil numbers during worm expulsion in nulliparous and lactating rats infected with Nippostrongylus brasiliensis. Int Arch Allergy Appl Immunol. 1972;43(4):497–509. doi: 10.1159/000230865. [DOI] [PubMed] [Google Scholar]
  13. LARSH J. E., Jr, GOULSON H. T., WEATHERLY N. F. STUDIES ON DELAYED (CELLULAR) HYPERSENSITIVITY IN MICE INFECTED WITH TRICHINELLA SPIRALIS. II. TRANSFER OF PERITONEAL EXUDATE CELLS. J Parasitol. 1964 Aug;50:496–498. [PubMed] [Google Scholar]
  14. MULLIGAN W., URQUHART G. M., JENNINGS F. W., NEILSON J. T. IMMUNOLOGICAL STUDIES ON NIPPOSTRONGYLUS BRASILIENSIS INFECTION IN THE RAT: "SELF-CURE" PHENOMENON. Exp Parasitol. 1965 Jun;16:341–347. doi: 10.1016/0014-4894(65)90056-1. [DOI] [PubMed] [Google Scholar]
  15. Murray M., Jarrett W. F., Jennings F. W. Mast cells and macromolecular leak in intestinal immunological reactions. The influence of sex of rats infected with Nippostrongylus brasiliensis. Immunology. 1971 Jul;21(1):17–31. [PMC free article] [PubMed] [Google Scholar]
  16. Ogilvie B. M., Hockley D. J. Effects of immunity of Nippostrongylus brasiliensis adult worms: reversible and irreversible changes in infectivity, reproduction, and morphology. J Parasitol. 1968 Dec;54(6):1073–1084. [PubMed] [Google Scholar]
  17. Ogilvie B. M., Jones V. E. Passive protection with cells or antiserum against Nippostronglylus brasiliensis in the rat. Parasitology. 1968 Nov;58(4):939–949. doi: 10.1017/s0031182000069705. [DOI] [PubMed] [Google Scholar]
  19. Urquhart G. M., Mulligan W., Eadie R. M., Jennings F. W. Immunological studies on Nippostrongylus brasiliensis infection in the rat: the role of local anaphylaxis. Exp Parasitol. 1965 Oct;17(2):210–217. doi: 10.1016/0014-4894(65)90023-8. [DOI] [PubMed] [Google Scholar]
  20. WELLS P. D. Mast cell, eosinophil and histamine levels in Nippostrongylus brasiliensis infected rats. Exp Parasitol. 1962 Apr;12:82–101. doi: 10.1016/s0014-4894(62)80002-2. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES