Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1973 Sep;25(3):459–469.

Effector activating determinants on IgG

I. The distribution and factors influencing the display of complement, neutrophil and cytotoxic B-cell determinants on human IgG sub-classes

I C M MacLennan, A Howard, Frances M Gotch, P G Quie
PMCID: PMC1423071  PMID: 4126353

Abstract

It was possible to demonstrate complement fixation by IgG1 and IgG3 sub-class myeloma proteins which had been heat aggregated under standard conditions. Phagocytosis of bacteria by neutrophils was inhibited by IgG1, IgG2 and IgG3. Lysis of antibody-sensitized target cells by cytotoxic B lymphocytes was inhibited by all four sub-classes of IgG.

IgG interacted with cytotoxic B lymphocytes, neutrophils and complement only after physical alteration. Cytotoxic B cell reacting activity was acquired during protein purification procedures and was only slightly increased by heating. Neutrophil inhibition activity appeared only after the IgG sub-classes were heated and aggregation had occurred. Very large aggregates, however, were inefficient inhibitory units. Complement fixation by IgG1 and IgG3 was markedly increased by heating and the largest aggregates showed greatest activity.

Full text

PDF
459

Images in this article

462FIG. 1
on p.462

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnett I. G., MacLennan I. C. Inhibitory effect of rheumatoid sera on cell damage by lymphocytes. Ann Rheum Dis. 1972 Sep;31(5):425–425. doi: 10.1136/ard.31.5.425-a. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Basten A., Sprent J., Miller J. F. Receptor for antibody-antigen complexes used to separate T cells from B cells. Nat New Biol. 1972 Feb 9;235(58):178–180. doi: 10.1038/newbio235178a0. [DOI] [PubMed] [Google Scholar]
  3. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  4. Frangione B., Milstein C., Pink J. R. Structural studies of immunoglobulin G. Nature. 1969 Jan 11;221(5176):145–148. doi: 10.1038/221145a0. [DOI] [PubMed] [Google Scholar]
  5. Greaves M. F. Biological effects of anti-immunoglobulins: evidence for immunoglobulin receptors on 'T' and 'B' lymphocytes. Transplant Rev. 1970;5:45–75. doi: 10.1111/j.1600-065x.1970.tb00356.x. [DOI] [PubMed] [Google Scholar]
  6. Humphrey J. H. Haemolytic efficiency of rabbit IgG anti-Forssman antibody and its augmentation by anti-rabbit IgG. Nature. 1967 Dec 30;216(5122):1295–1296. doi: 10.1038/2161295a0. [DOI] [PubMed] [Google Scholar]
  7. Hyslop N. E., Jr, Dourmashkin R. R., Green N. M., Porter R. R. The fixation of complement and the activated first component (C1) of complement by complexes formed between antibody and divalent hapten. J Exp Med. 1970 Apr 1;131(4):783–802. doi: 10.1084/jem.131.4.783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ishizaka T., Ishizaka K., Salmon S., Fudenberg H. Biologic activities of aggregated gamma-globulin. 8. Aggregated immunoglobulins of different classes. J Immunol. 1967 Jul;99(1):82–91. [PubMed] [Google Scholar]
  9. MacLennan I. C. Antibody in the induction and inhibition of lymphocyte cytotoxicity. Transplant Rev. 1972;13:67–90. doi: 10.1111/j.1600-065x.1972.tb00060.x. [DOI] [PubMed] [Google Scholar]
  10. MacLennan I. C., Howard A. Evidence for correlation between the antigenic specificity and charge of human IgG. A study of antibody-inducing lymphocyte mediated cell damage. Immunology. 1972 Jun;22(6):1043–1049. [PMC free article] [PubMed] [Google Scholar]
  11. MacLennan I. C., Loewi G., Harding B. The role of immunoglobulins in lymphocyte-mediated cell damage, in vitro. I. Comparison of the effects of target cell specific antibody and normal serum factors on cellular damage by immune and non-immune lymphocytes. Immunology. 1970 Mar;18(3):397–404. [PMC free article] [PubMed] [Google Scholar]
  12. Messner R. P., Jelinek J. Receptors for human gamma G globulin on human neutrophils. J Clin Invest. 1970 Dec;49(12):2165–2171. doi: 10.1172/JCI106435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Plaut A. G., Cohen S., Tomasi T. B., Jr Immunoglobulin M: fixation of human complement by the Fc fragment. Science. 1972 Apr 7;176(4030):55–56. doi: 10.1126/science.176.4030.55. [DOI] [PubMed] [Google Scholar]
  14. Reid K. B. Complement fixation by the F(ab')2-fragment of pepsin-treated rabbit antibody. Immunology. 1971 May;20(5):649–658. [PMC free article] [PubMed] [Google Scholar]
  15. Terry W. D. Skin-sensitizing activity related to gamma- polypeptide chain characteristics of human IgG. J Immunol. 1965 Dec;95(6):1041–1047. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES