Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1974 Mar;26(3):455–463.

Non-precipitating guinea-pig antibodies produced by administration of excessive doses of ovalbumin

K Tamoto, T Nakamura, J Koyama
PMCID: PMC1423218  PMID: 4853362

Abstract

Partial paralysis of the antibody response in guinea-pigs produced by administration of an excessive dose of ovalbumin was accompanied by the formation of non-precipitating IgG1 and IgG2 antibodies. Immunochemical properties of these non-precipitating antibodies were compared with those of the precipitating antibodies obtained from animals given a normal dose of ovalbumin. Non-precipitating IgG2 antibodies did not fix complement and non-precipitating IgG1 antibodies elicited PCA but with less efficiency than precipitating IgG1 antibodies. On the other hand, the ammonium sulphate globulin precipitation technique for the determination of antibody affinity revealed that precipitating and non-precipitating antibodies were similar with regard to valency and average affinity for the antigen. These results are compatible with the hypothesis that non-precipitating antibodies result from a limited recognition of multiple antigenic determinants on ovalbumin molecules.

Full text

PDF
455

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTIN P. Coexistence de complexes précipitants et non-précipitants dans la réaction entre la sérum-albumine humaine et les immunsérums spécifiques de chevaux; déductions quantitatives et conséquences théoriques. Bull Soc Chim Biol (Paris) 1955;37(9-10):977–994. [PubMed] [Google Scholar]
  2. Carter B. G., Harris T. N. Non-precipitating rabbit antibody to hapten: purification and properties. Immunology. 1967 Jan;12(1):75–88. [PMC free article] [PubMed] [Google Scholar]
  3. Christian C. L. Character of non-precipitating antibodies. Immunology. 1970 Apr;18(4):457–466. [PMC free article] [PubMed] [Google Scholar]
  4. FARR R. S. A quantitative immunochemical measure of the primary interaction between I BSA and antibody. J Infect Dis. 1958 Nov-Dec;103(3):239–262. doi: 10.1093/infdis/103.3.239. [DOI] [PubMed] [Google Scholar]
  5. FISET L. G. Non-precipitating rabbit antibodies: a study of their reactivity in vitro by means of the quantitative precipitin and tannic acid haemagglutination techniques. Immunology. 1962 Sep;5:580–594. [PMC free article] [PubMed] [Google Scholar]
  6. GROSSBERG A. L., RADZIMSKI G., PRESSMAN D. Effect of iodination on the active site of several antihapten antibodies. Biochemistry. 1962 May 25;1:391–401. doi: 10.1021/bi00909a004. [DOI] [PubMed] [Google Scholar]
  7. Herbert W. J. The preparation of antigenically defined conalbumin-free ovalbumin, by ion-exchange chromatography. Immunochemistry. 1967 May;4(3):143–150. doi: 10.1016/0019-2791(67)90123-1. [DOI] [PubMed] [Google Scholar]
  8. Kekwick R. A., Cannan R. K. The hydrogen ion dissociation curve of the crystalline albumin of the hen's egg. Biochem J. 1936 Feb;30(2):227–234. doi: 10.1042/bj0300227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mancino D., Tigelaar R. E., Ovary Z. Passive cutaneous anaphylactic and Schultz-Dale reactions elicited in guinea pigs by chemically univalent dinitrophenyl-derivatives of bacitracin A and papain. J Immunol. 1969 Oct;103(4):750–756. [PubMed] [Google Scholar]
  10. Margni R., Binaghi R. Purification and properties of non-precipitating rabbit antibodies. Immunology. 1972 Apr;22(4):557–563. [PMC free article] [PubMed] [Google Scholar]
  11. Nakamura T., Ofune K., Tamoto K., Koyama J. Immunochemical studies on guinea pig non-precipitating antibodies to ovalbumin. J Biochem. 1972 Feb;71(2):245–256. doi: 10.1093/oxfordjournals.jbchem.a129761. [DOI] [PubMed] [Google Scholar]
  12. Nakamura T., Okawa T., Koyama J. Effect of varying the immunizing dose of ovalbumin on the production of non-precipitating IgG1 and IgG2 antibodies in guinea pigs. J Biochem. 1973 Jul;74(1):23–32. doi: 10.1093/oxfordjournals.jbchem.a130227. [DOI] [PubMed] [Google Scholar]
  13. OVARY Z., BIER O. G. Quantitative studies on passive cutaneous anaphylaxis in the guinea pig and its relationship to the Arthus phenomenon. J Immunol. 1953 Jul;71(1):6–11. [PubMed] [Google Scholar]
  14. Tamoto K., Nakamura T., Koyama J. Further studies on guinea pig non-precipitating 7S 2 -antibodies to ovalbumin. J Biochem. 1972 Aug;72(2):261–268. doi: 10.1093/oxfordjournals.jbchem.a129905. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES