Abstract
HgA-negative rabbits were given an intravenous injection of HgA-positive red cells followed within 30 minutes by an intravenous injection of IgG anti-HgA. Several weeks later a second injection of HgA-positive red cells, without antibody, was given; the survival of these red cells was compared with the survival of red cells given to animals which had received no passive antibody. As judged by a relatively good survival of the second injection of red cells, primary immunization to HgA was at least partially suppressed when the dose of anti-HgA given with the first injection of red cells was such that 260–750 μg antibody was bound per m1 red cells. In three groups of animals given HgA-positive cells and anti-HgA in doses such that 20–40 μg antibody was bound per m1 red cells there was apparently no suppression of the antibody response.
It is concluded that the amount of specific IgG antibody required for the suppression of primary immunization is at least ten times greater, in terms of μg antibody per m1 red cells, for the rabbit antigen HgA than for the human antigen Rh; the difference may be due partly to the greater number of HgA antigen sites on the rabbit red cell compared with the number of Rh(D) sites on the human red cell.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Burton M. S., Mollison P. L. Effect of IgM and IgG iso-antibody on red cell clearance. Immunology. 1968 Jun;14(6):861–878. [PMC free article] [PubMed] [Google Scholar]
- EISEN H. N., SISKIND G. W. VARIATIONS IN AFFINITIES OF ANTIBODIES DURING THE IMMUNE RESPONSE. Biochemistry. 1964 Jul;3:996–1008. doi: 10.1021/bi00895a027. [DOI] [PubMed] [Google Scholar]
- Elson C. J., Woodrow J. C., Donohoe W. T. Clearance of erythrocytes and the immune response. An experimental study. Vox Sang. 1971 Mar;20(3):201–208. doi: 10.1111/j.1423-0410.1971.tb00431.x. [DOI] [PubMed] [Google Scholar]
- Frame M., Mollison P. L. The rabbit red cell antigen Hg A and anti-Hg A . Immunology. 1972 Jun;22(6):1037–1042. [PMC free article] [PubMed] [Google Scholar]
- Mollison P. L. The effect of isoantibodies on red-cell survival. Ann N Y Acad Sci. 1970 Feb 13;169(1):199–216. doi: 10.1111/j.1749-6632.1970.tb55987.x. [DOI] [PubMed] [Google Scholar]
- Pollack W., Gorman J. G., Hager H. J., Freda V. J., Tripodi D. Antibody-mediated immune suppression to the Rh factor: animal models suggesting mechanism of action. Transfusion. 1968 May-Jun;8(3):134–145. doi: 10.1111/j.1537-2995.1968.tb04891.x. [DOI] [PubMed] [Google Scholar]
- Smith G. N., Mollison P. L. Responses in rabbits to the red cell alloantigen HgA. Immunology. 1974 May;26(5):865–875. [PMC free article] [PubMed] [Google Scholar]
- Smith G. N., Mollison P. L. The immune response of HgA-negative rabbits to HgA-positive red cells treated with N-ethylmaleimide. Immunology. 1974 May;26(5):877–883. [PMC free article] [PubMed] [Google Scholar]