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. 1964 Jan;7(1):40–53.

An in vitro system for the study of the mechanism of antigenic stimulation in the secondary response

R W Dutton, Jennifer D Eady
PMCID: PMC1423299  PMID: 14113075

Abstract

The uptake of [14C]thymidine by spleen cell suspensions from rabbits previously immunized to heterologous serum proteins is stimulated by the addition of antigen in vitro. This stimulation is specific and dependent upon concentration of antigen. It represents an increased rate of DNA synthesis and cell division by certain cells of the suspension. These cells are large undifferentiated mononuclear cells comprising approximately 1 per cent of the total population. It is suggested that this response is the in vitro counterpart of the cellular proliferation seen in whole animals following the re-injection of antigen. The procedure described provides a quantitative system for the study of the mechanism of antigenic stimulation. Some preliminary experiments are reported.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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