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. 1974 Jun;26(6):1195–1206.

Immunofluorescence and elution studies in tetraparental NZB↔CFW chimaeras and graft-versus-host diseased NZB

R D Barnes, Jean Holliday, Maureen Tuffrey
PMCID: PMC1423358  PMID: 4368790

Abstract

Earlier evidence in these chimaeras suggested an acute interaction between the two parental strains in all of four ovum fusion-derived tetraparental NZB↔CFW chimaeras. In spite of this acute interaction indirect immunofluorescence upon unfixed tissue sections has here confirmed the presence of both H-2 defined parental strain cell populations in each mouse. This supports our earlier view that the acute reaction resulted from two separate events, and the evidence suggests that these events are the opposite of those in NZB injected with CFW spleen cells. In these graft-versus-host (GVH) diseased mice two separate events occurred. Firstly a reaction of the CFW spleen cells against histocompatibility antigens of the NZB, secondly the response of NZB host against the donor cells (viz. NZB—anti-CFW). In the chimaeras the primary reaction involved NZB autoantibody activity against the corresponding target. This was initiated and followed by a secondary CFW—anti-NZB response. This sequence of receptor→ determinant reactions is held responsible for the acute allogeneic activity in these chimaeras.

Marked erythrophagocytosis was seen in all of the NZB↔CFW chimaeras. Immunofluorescence has revealed immunoglobulin, both allotypes, complement (MuB1) and H-2 antigens in similar cells with red cell-like inclusions. Evidence suggests that erythrophagocytosis in this situation is perhaps also dependent and initiated by the same receptor→determinant reaction. This in turn argues the presence of the corresponding receptors upon the phagocytic macrophages.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison A. C., Denman A. M., Barnes R. D. Cooperating and controlling functions of thymus-derived lymphocytes in relation to autoimmunity. Lancet. 1971 Jul 17;2(7716):135–140. doi: 10.1016/s0140-6736(71)92306-3. [DOI] [PubMed] [Google Scholar]
  2. Barnes R. D., Tuffrey M., Kingman J., Risdon R. A. The disease of the NZB mouse. Examination of exchange ovum transplantation derived NZB and CFW mice. Clin Exp Immunol. 1972 Mar;10(3):493–514. [PMC free article] [PubMed] [Google Scholar]
  3. Barnes R. D., Tuffrey M., Kingman J., Thornton C., Turner M. W. The disease of the NZB mouse. I. Examination of ovum fusion derived tetraparental NZB:CFW chimaeras. Clin Exp Immunol. 1972 Aug;11(4):605–628. [PMC free article] [PubMed] [Google Scholar]
  4. CINADER B., DUBISKI S., WARDLAW A. C. DISTRIBUTION, INHERITANCE, AND PROPERTIES OF AN ANTIGEN, MUB1, AND ITS RELATION TO HEMOLYTIC COMPLEMENT. J Exp Med. 1964 Nov 1;120:897–924. doi: 10.1084/jem.120.5.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ceska M. Immuno-precipitates of some purified and unpurified human plasma proteins with monospecific and polyvalent human plasma protein antisera. J Immunol. 1969 Jul;103(1):100–106. [PubMed] [Google Scholar]
  6. Gengozian N., Batson J. S., Greene C. T., Gosslee D. G. Hemopoietic chimerism in imported and laboratory-bred marmosets. Transplantation. 1969 Nov;8(5):633–652. doi: 10.1097/00007890-196911000-00009. [DOI] [PubMed] [Google Scholar]
  7. Gervais A. G. Detection of mouse histocompatibility antigens by immunofluorescence. Transplantation. 1968 Mar;6(2):261–276. [PubMed] [Google Scholar]
  8. Lambert P. H., Dixon F. J. Pathogenesis of the glomerulonephritis of NZB/W mice. J Exp Med. 1968 Mar 1;127(3):507–522. doi: 10.1084/jem.127.3.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lewis R. M., Armstrong M. Y., André-Schwartz J., Muftuoglu A., Beldotti L., Schwartz R. S. Chronic allogeneic disease. I. Development of glomerulonephritis. J Exp Med. 1968 Oct 1;128(4):653–679. doi: 10.1084/jem.128.4.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mintz B., Palm J. Gene control of hematopoiesis. I. Erythrocyte mosaicism and permanent immunological tolerance in allophenic mice. J Exp Med. 1969 May 1;129(5):1013–1027. doi: 10.1084/jem.129.5.1013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mintz B., Silvers W. K. "Intrinsic" immunological tolerance in allophenic mice. Science. 1967 Dec 15;158(3807):1484–1486. doi: 10.1126/science.158.3807.1484. [DOI] [PubMed] [Google Scholar]
  12. STONE W. H., FRIEDMAN J., FREGIN A. POSSIBLE SOMATIC CELL MATING IN TWIN CATTLE WITH ERYTHROCYTE MOSAICISM. Proc Natl Acad Sci U S A. 1964 Jun;51:1036–1044. doi: 10.1073/pnas.51.6.1036. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. TAN E. M., KAPLAN M. H. IMMUNOLOGICAL RELATION OF BASEMENT MEMBRANE AND A SERUM BETA GLOBULIN IN THE MOUSE. DEMONSTRATION OF RENAL BASEMENT MEMBRANE ALTERATION IN MICE INJECTED WITH STREPTOLYSIN S. Immunology. 1963 Jul;6:331–344. [PMC free article] [PubMed] [Google Scholar]
  14. Taylor R. B. Cellular cooperation in the antibody response of mice to two serum albumins: specific function of thymus cells. Transplant Rev. 1969;1:114–149. doi: 10.1111/j.1600-065x.1969.tb00138.x. [DOI] [PubMed] [Google Scholar]

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