Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1965 Apr;8(4):420–431.

The reaction with red cells of 7S rabbit antibody, its sub-units and their recombinants

C L Greenbury, D H Moore, L A C Nunn
PMCID: PMC1423495  PMID: 14279047

Abstract

Univalent 3.5S antibody fragments obtained from 7S rabbit antibody specific for human group A red cells, have a very much smaller association constant in their reaction with human group A1 red cells than have 7S or 5S antibody. Univalent 5S recombinants have a similarly low association constant, whereas bivalent 5S recombinants have an association constant similar to that of 7S antibody.

It is concluded that this supports the authors' hypothesis that rabbit anti-A 7S antibody is normally attached to red cells by both its specific antibody groups.

Full text

PDF
420

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALMEIDA J., CINADER B., HOWATSON A. THE STRUCTURE OF ANTIGEN-ANTIBODY COMPLEXES. A STUDY BY ELECTRON MICROSCOPY. J Exp Med. 1963 Sep 1;118:327–340. doi: 10.1084/jem.118.3.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fudenberg H., Mandy W. J., Nisonoff A. SEROLOGIC STUDIES OF PROTEOLYTIC FRAGMENTS OF RABBIT AGGLUTINATING ANTIBODIES. J Clin Invest. 1962 Dec;41(12):2123–2134. doi: 10.1172/JCI104670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GREENBURY C. L., MOORE D. H., NUNN L. A. COMBINATION WITH RED CELLS OF IMMUNE RABBIT 7S ANTIBODY AND ITS SUB-UNITS. Nature. 1964 Sep 12;203:1147–1148. doi: 10.1038/2031147a0. [DOI] [PubMed] [Google Scholar]
  4. McFarlane A. S. IN VIVO BEHAVIOR OF I-FIBRINOGEN. J Clin Invest. 1963 Mar;42(3):346–361. doi: 10.1172/JCI104721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. NISONOFF A., MANDY W. J. Quantitative estimation of the hybridization of rabbit antibodies. Nature. 1962 Apr 28;194:355–359. doi: 10.1038/194355a0. [DOI] [PubMed] [Google Scholar]
  6. NISONOFF A., WISSLER F. C., LIPMAN L. N., WOERNLEY D. L. Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulfide bonds. Arch Biochem Biophys. 1960 Aug;89:230–244. doi: 10.1016/0003-9861(60)90049-7. [DOI] [PubMed] [Google Scholar]
  7. NISONOFF A., WOERNLEY D. L. Effect of hydrolysis by papain on the combining sites of an antibody. Nature. 1959 May 9;183(4671):1325–1326. doi: 10.1038/1831325a0. [DOI] [PubMed] [Google Scholar]
  8. PORTER R. R. The hydrolysis of rabbit y-globulin and antibodies with crystalline papain. Biochem J. 1959 Sep;73:119–126. doi: 10.1042/bj0730119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. STELOS P., RADZIMSKI G., PRESSMAN D. Heterogeneity of rabbit antibody fragments. J Immunol. 1962 May;88:572–580. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES