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. 1965 Oct;9(4):391–401.

Formation of specific antibody by circulating cells

Lotte Hulliger, E Sorkin
PMCID: PMC1423591  PMID: 5844811

Abstract

The rate of antibody synthesis as measured by 14C-amino acid incorporation was estimated in thoracic duct lymph, buffy coat and spleen cells from rabbits hyperimmunized and boosted with human serum albumin (HSA). Cells from thoracic duct and buffy coat synthesized antibody in most experiments in small amounts, in two experiments in larger amounts. Spleen cells gave the highest values but also showed considerable variation from animal to animal.

The comparison of antibody synthesis with the cellular composition of the different cell preparations led to the conclusion that the small percentage of pyroninophilic cells in lymph and buffy coat are responsible for the antibody synthesis and not the bulk of lymphocytes.

Cell-bound antibody measured by fixation of radioactive HSA was found in spleen lymph and buffy coat cells. The major part of the detected cell-fixed antibody is presumably present on the surface of and within antibody-producing cells and not due to passively adsorbed cytophilic antibody on non-antibody-producing cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASKONAS B. A., HUMPHREY J. H. Formation of specific antibodies and gamma-globulin in vitro; a study of the synthetic ability of various tissues from rabbits immunized by different methods. Biochem J. 1958 Feb;68(2):252–261. doi: 10.1042/bj0680252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BOYDEN S. V., SORKIN E. The adsorption of antigen by spleen cells previously treated with antiserum in vitro. Immunology. 1960 Jul;3:272–283. [PMC free article] [PubMed] [Google Scholar]
  3. FORBES I. J. SPECIFIC AND NON-SPECIFIC STIMULATION OF ANTIBODY SYNTHESIS BY HUMAN LEUCOCYTES IN VITRO. Lancet. 1965 Jan 23;1(7378):198–199. doi: 10.1016/s0140-6736(65)90979-7. [DOI] [PubMed] [Google Scholar]
  4. GOWANS J. L., McGREGOR D. D., COWEN D. M. Initiation of immune responses by small lymphocytes. Nature. 1962 Nov 17;196:651–655. doi: 10.1038/196651a0. [DOI] [PubMed] [Google Scholar]
  5. HIRSCHHORN K., BACH F., KOLODNY R. L., FIRSCHEIN I. L., HASHEM N. IMMUNE RESPONSE AND MITOSIS OF HUMAN PERIPHERAL BLOOD LYMPHOCYTES IN VITRO. Science. 1963 Nov 29;142(3596):1185–1187. doi: 10.1126/science.142.3596.1185. [DOI] [PubMed] [Google Scholar]
  6. HULLIGER L., SORKIN E. Synthesis of antibodies by blood leucocytes of the rabbit. Nature. 1963 Apr 20;198:299–299. doi: 10.1038/198299a0. [DOI] [PubMed] [Google Scholar]
  7. HULLIGER L. Uber die unterschiedlichen Entwicklungsfähigkeiten der Zellen des Blutes und der Lymphe in vitro. Virchows Arch Pathol Anat Physiol Klin Med. 1956;329(3):289–318. doi: 10.1007/BF00955132. [DOI] [PubMed] [Google Scholar]
  8. LANDY M., SANDERSON R. P., BERNSTEIN M. T., JACKSON A. L. ANTIBODY PRODUCTION BY LEUCOCYTES IN PERIPHERAL BLOOD. Nature. 1964 Dec 26;204:1320–1321. doi: 10.1038/2041320a0. [DOI] [PubMed] [Google Scholar]
  10. SORKIN E. CELL-BOUND AND CELL-BINDING ANTIBODY IN THE PERIPHERAL BLOOD OF IMMUNIZED RABBITS. Nature. 1964 Nov 21;204:794–795. doi: 10.1038/204794a0. [DOI] [PubMed] [Google Scholar]

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