Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1965 Aug;9(2):139–150.

The reactivity of rheumatoid factor with human gamma G globulin

C S Henney, D R Stanworth
PMCID: PMC1423699  PMID: 4158318

Abstract

The precipitation reaction between denatured human γG globulin and rheumatoid arthritic serum has been employed in quantitative form to provide further indication of the antibody nature of rheumatoid factor.

In addition, ultracentrifugal and optical rotation studies have revealed that the degree of reactivity of the γG globulin in such a system depends on its extent of denaturation irrespective of the type of denaturing agent (heat, alkali, detergent) employed.

Evidence is presented to suggest that denaturation processes responsible for effecting the precipitation reactivity of human γG globulin (with rheumatoid factor) bring about the rupture of inter-chain disulphide bonds in the `fast' papain digestion (i.e. Fc) parts of the molecules and these take part in intermolecular bridging, leading to aggregate formation and probably to the exposure of new chemical groupings.

Full text

PDF

Images in this article

143FIG. 4
on p.143
146FIG. 7
on p.146
146FIG. 8
on p.146

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHRISTIAN C. L. Characterization of the reactant (gamma globulin factor) in the F II precipitin reaction and the F II tanned sheep cell agglutination test. J Exp Med. 1958 Jul 1;108(1):139–157. doi: 10.1084/jem.108.1.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. EDELMAN G. M., KUNKEL H. G., FRANKLIN E. C. Interaction of the rheumatoid factor with antigen-antibody complexes and aggregated gamma globulin. J Exp Med. 1958 Jul 1;108(1):105–120. doi: 10.1084/jem.108.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GELL P. G. The estimation of the individual human serum proteins by an immunological method. J Clin Pathol. 1957 Feb;10(1):67–71. doi: 10.1136/jcp.10.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GLYNN L. E., HOLBOROW E. J., JOHNSON G. D. The nature of the Rose-Waaler factor. Proc R Soc Med. 1957 Jul;50(7):469–471. [PubMed] [Google Scholar]
  5. HENNEY C. S., STANWORTH D. R. REACTION OF RHEUMATOID FACTOR WITH THE ISOLATED POLYPEPTIDE CHAINS OF HUMAN 7S GAMMA-GLOBULIN. Nature. 1964 Feb 1;201:511–512. doi: 10.1038/201511a0. [DOI] [PubMed] [Google Scholar]
  6. Henney C. S., Stanworth D. R., Gell P. G. Demonstration of the exposure of new antigenic determinants following antigen-antibody combination. Nature. 1965 Mar 13;205(976):1079–1081. doi: 10.1038/2051079a0. [DOI] [PubMed] [Google Scholar]
  7. ISHIZAKA K., CAMPBELL D. H. Biologic activity of soluble antigen-antibody complexes. V. Change of optical rotation by the formation of skin reactive complexes. J Immunol. 1959 Sep;83:318–326. [PubMed] [Google Scholar]
  8. ISHIZAKA K., ISHIZAKA T. MOLECULAR BASIS OF PASSIVE SENSITIZATION. II. THE ROLE OF FRAGMENT 3 OF GAMMA-GLOBULIN AND ITS DISULFIDE BONDS IN PASSIVE SENSITIZATION AND COMPLEMENT FIXATION. J Immunol. 1964 Jul;93:59–68. [PubMed] [Google Scholar]
  9. JAMES K., FELIX-DAVIES D., STANWORTH D. R. Studies on the isolation of rheumatoid factor. Ann Rheum Dis. 1961 Dec;20:369–385. doi: 10.1136/ard.20.4.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. JAMES K., HENNEY C. S., STANWORTH D. R. STRUCTURAL CHANGES OCCURRING IN 7S GAMMA-GLOBULINS. Nature. 1964 May 9;202:563–566. doi: 10.1038/202563a0. [DOI] [PubMed] [Google Scholar]
  11. JOCELYN P. C. The effect of glutathione on protein sulphydryl groups in rat-liver homogenates. Biochem J. 1962 Dec;85:480–485. doi: 10.1042/bj0850480. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. MILGROM F., WITEBSKY E. Studies on the rheumatoid and related serum factors. I. Autoimmunization of rabbits with gamma globulin. JAMA. 1960 Sep 3;174:56–63. doi: 10.1001/jama.1960.63030010013013. [DOI] [PubMed] [Google Scholar]
  14. ORESKES I., SINGER J. M., PLOTZ C. M. Reactivity of rheumatoid arthritis sera with altered and aggregated human gamma-globulin. J Immunol. 1963 Jan;90:107–115. [PubMed] [Google Scholar]
  15. PALMER J. L., NISONOFF A. DISSOCIATION OF RABBIT GAMMA-GLOBULIN INTO HALF-MOLECULES AFTER REDUCTION OF ONE LABILE DISULFIDE BOND. Biochemistry. 1964 Jun;3:863–869. doi: 10.1021/bi00894a024. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES