Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1965 Nov;9(5):483–490.

The short-term culture of lymphoid tissue from immunized guinea-pigs

Ann M Dresser
PMCID: PMC1423717  PMID: 5847427

Abstract

Lymph node tissue from guinea-pigs immunized against one of several antigens was cultured in different media in order to determine conditions under which maximal amounts of antibody are formed in vitro. The amounts of antibody formed were variable and bore no relation to the level of serum antibody in the tissue donor. Heterologous sera of several origins, when included in the culture medium, varied very markedly in their capacity to support antibody production in vitro.

Full text

PDF
483

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMBROSE C. T. THE REQUIREMENT FOR HYDROCORTISONE IN ANTIBODY-FORMING TISSUE CULTIVATED IN SERUM-FREE MEDIUM. J Exp Med. 1964 Jan 1;119:1027–1049. doi: 10.1084/jem.119.6.1027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ASKONAS B. A., WHITE R. G. Sites of antibody production in the guinea-pig; the relation between in vitro synthesis of anti-ovalbumin and gamma-globulin and distribution of antibody-containing plasma cells. Br J Exp Pathol. 1956 Feb;37(1):61–74. [PMC free article] [PubMed] [Google Scholar]
  3. ATTARDI G., COHN M., HORIBATA K., LENNOX E. S. ANTIBODY FORMATION BY RABBIT LYMPH NODE CELLS. IV. THE DETAILED METHODS FOR MEASURING ANTIBODY SYNTHESIS BY INDIVIDUAL CELLS, THE KINETICS OF ANTIBODY FORMATION BY RABBITS AND THE PROPERTIES OF CELL SUSPENSIONS. J Immunol. 1964 Mar;92:372–390. [PubMed] [Google Scholar]
  4. CAILLEAU R., KIRK P. L. Some factors affecting the growth-promoting properties of serum in tissue culture. Tex Rep Biol Med. 1957;15(2):237–249. [PubMed] [Google Scholar]
  5. DINEEN J. K. SOURCES OF IMMUNOLOGICAL VARIATION. Nature. 1964 Apr 4;202:101–102. doi: 10.1038/202101a0. [DOI] [PubMed] [Google Scholar]
  6. IPSEN J., Jr Inherent immunizability to tetanus toxoid, based on studies in pure inbred mice. J Immunol. 1954 Apr;72(4):243–247. [PubMed] [Google Scholar]
  7. KESTON A. S., KATCHEN B. Incorporation of glycine-2-C14 into homologous antibody by rabbit tissue slices. J Immunol. 1956 Apr;76(4):253–258. [PubMed] [Google Scholar]
  8. MANS R. J., NOVELLI G. D. A convenient, rapid and sensitive method for measuring the incorporation of radioactive amino acids into protein. Biochem Biophys Res Commun. 1960 Nov;3:540–543. doi: 10.1016/0006-291x(60)90171-6. [DOI] [PubMed] [Google Scholar]
  9. MCKENNA J. M., STEVENS K. M. The early phase of the antibody response. J Immunol. 1957 May;78(5):311–317. [PubMed] [Google Scholar]
  10. MICHL J. Metabolism of cells in tissue culture in vitro. I. The influence of serum protein fractions on the growth of normal and neoplastic cells. Exp Cell Res. 1961 Mar;23:324–334. doi: 10.1016/0014-4827(61)90042-8. [DOI] [PubMed] [Google Scholar]
  11. PHILLIPS H. J., ANDREWS R. V. Some protective solutions for tissue, cultured cells. Exp Cell Res. 1959 Mar;16(3):678–682. doi: 10.1016/0014-4827(59)90136-3. [DOI] [PubMed] [Google Scholar]
  12. RANNEY H. M., LONDON I. M. Antibody formation in surviving tissues. Fed Proc. 1951 Jun;10(2):562–563. [PubMed] [Google Scholar]
  13. STAVITSKY A. B. In vitro production of diphtheria antitoxin by tissues of immunized animals. I. Procedure and evidence for general nature of phenomenon. J Immunol. 1955 Sep;75(3):214–224. [PubMed] [Google Scholar]
  14. Steiner D. F., Anker H. S. ON THE SYNTHESIS OF ANTIBODY PROTEIN IN VITRO. Proc Natl Acad Sci U S A. 1956 Sep;42(9):580–586. doi: 10.1073/pnas.42.9.580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. TOZER B. T., PIRT S. J. SUSPENSION CULTURE OF MAMMALIAN CELLS AND MACROMOLECULAR GROWTH-PROMOTING FRACTIONS OF CALF SERUM. Nature. 1964 Jan 25;201:375–378. doi: 10.1038/201375a0. [DOI] [PubMed] [Google Scholar]
  16. TROWELL O. A. The culture of lymph nodes in synthetic media. Exp Cell Res. 1955 Oct;9(2):258–276. doi: 10.1016/0014-4827(55)90099-9. [DOI] [PubMed] [Google Scholar]
  17. VAUGHAN J. H., DUTTON A. H., DUTTON R. W., GEORGE M., MARSTON R. Q. A study of antibody production in vitro. J Immunol. 1960 Mar;84:258–267. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES