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. 1966 Jun;10(6):549–557.

Recombination of heavy and light chains from human immunoglobulins

S Gordon, S Cohen
PMCID: PMC1423733  PMID: 4161807

Abstract

The recombination of reduced, alkylated heavy and light chains from three IgG, two IgM and one IgA pathological protein and from normal IgG has been quantitatively studied.

All γ, α and μ chains showed some recombination with all κ and λ chains tested. One of the IgG and two of the IgM proteins showed more than 50 per cent recombination and in these there was evidence of a selective reconstitution of autologous chain pairs; the structural features which determine re-association appear to be independent of the electrophoretic mobility, or antigenic and allotypic specificity of the interacting chains. The chains of normal IgG and of three myeloma proteins showed a low percentage recombination and these autologous chain pairs did not combine preferentially. It appears that immunoglobulin chains show a variable ability to regain their native configuration after isolation.

Two pathological κ chains which were identical in electrophoretic mobility and Inv specificity but not equivalent in re-association behaviour showed well marked differences in tryptic peptide patterns. This indicates that the number of chemically distinct human light chains exceeds the forty variants previously postulated on the basis of antigenic, allotypic and electrophoretic differences.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ATFIELD G. N., MORRIS C. J. Analytical separations by highvoltage paper electrophoresis. Amino acids in protein hydrolysates. Biochem J. 1961 Dec;81:606–614. doi: 10.1042/bj0810606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CHAPLIN H., COHEN S., PRESS E. M. PREPARATION AND PROPERTIES OF THE PEPTIDE CHAINS OF NORMAL HUMAN 19 S GAMMA-GLOBULIN (IGM). Biochem J. 1965 Apr;95:256–261. doi: 10.1042/bj0950256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. COHEN S., FREEMAN T. Metabolic heterogeneity of human gamma-globulin. Biochem J. 1960 Sep;76:475–487. doi: 10.1042/bj0760475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cohen S., Gordon S. Dissociation of kappa- and lambda-chains from reduced human immunoglobulins. Biochem J. 1965 Nov;97(2):460–465. doi: 10.1042/bj0970460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cohen S., Porter R. R. Heterogeneity of the peptide chains of gamma-globulin. Biochem J. 1964 Feb;90(2):278–284. doi: 10.1042/bj0900278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. EDELMAN G. M., OLINS D. E., GALLY J. A., ZINDER N. D. RECONSTITUTION OF IMMUNOLOGIC ACTIVITY BY INTERACTION OF POLYPEPTIDE CHAINS OF ANTIBODIES. Proc Natl Acad Sci U S A. 1963 Oct;50:753–761. doi: 10.1073/pnas.50.4.753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FLEISCHMAN J. B., PAIN R. H., PORTER R. R. Reduction of gamma-globulins. Arch Biochem Biophys. 1962 Sep;Suppl 1:174–180. [PubMed] [Google Scholar]
  8. FOUGEREAU M., EDELMAN G. M. RESEMBLANCE OF THE GROSS ARRANGEMENT OF POLYPEPTIDE CHAINS IN RECONSTITUTED AND NATIVE GAMMA-GLOBULINS. Biochemistry. 1964 Aug;3:1120–1126. doi: 10.1021/bi00896a019. [DOI] [PubMed] [Google Scholar]
  9. FOUGEREAU M., OLINS D. E., EDELMAN G. M. RECONSTITUTION OF ANTIPHAGE ANTIBODIES FROM L AND H POLYPEPTIDE CHAINS AND THE FORMATION OF INTERSPECIES MOLECULAR HYBRIDS. J Exp Med. 1964 Sep 1;120:349–358. doi: 10.1084/jem.120.3.349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GALLY J. A., EDELMAN G. M. PROTEIN-PROTEIN INTERACTIONS AMONG L POLYPEPTIDE CHAINS OF BENCE-JONES PROTEINS AND HUMAN GAMMA-GLOBULINS. J Exp Med. 1964 May 1;119:817–836. doi: 10.1084/jem.119.5.817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Grey H. M., Mannik M. Specificity of recombination of H and L chains from human gamma-G-myeloma proteins. J Exp Med. 1965 Sep 1;122(3):619–632. doi: 10.1084/jem.122.3.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hilschmann N., Craig L. C. Amino acid sequence studies with Bence-Jones proteins. Proc Natl Acad Sci U S A. 1965 Jun;53(6):1403–1409. doi: 10.1073/pnas.53.6.1403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KOSTKA V., CARPENTER F. H. INHIBITION OF CHYMOTRYPSIN ACTIVITY IN CRYSTALLINE TRYPSIN PREPARATIONS. J Biol Chem. 1964 Jun;239:1799–1803. [PubMed] [Google Scholar]
  14. KUNKEL H. G., ALLEN J. C., GREY H. M., MARTENSSON L., GRUBB R. A RELATIONSHIP BETWEEN THE H CHAIN GROUPS OF 7S GAMMA-GLOBULIN AND THE GM SYSTEM. Nature. 1964 Jul 25;203:413–414. doi: 10.1038/203413a0. [DOI] [PubMed] [Google Scholar]
  15. LAWLER S. D., COHEN S. DISTRIBUTION OF ALLOTYPIC SPECIFICITIES ON THE PEPTIDE CHAINS OF HUMAN GAMMA-GLOBULIN. Immunology. 1965 Feb;8:206–212. [PMC free article] [PubMed] [Google Scholar]
  16. METZGER H., MANNIK M. RECOMBINATION OF ANTIBODY POLYPEPTIDE CHAINS IN THE PRESENCE OF ANTIGEN. J Exp Med. 1964 Nov 1;120:765–782. doi: 10.1084/jem.120.5.765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. MILLER F., METZGER H. CHARACTERIZATION OF A HUMAN MACROGLOBULIN. I. THE MOLECULAR WEIGHT OF ITS SUBUNIT. J Biol Chem. 1965 Aug;240:3325–3333. [PubMed] [Google Scholar]
  18. MILSTEIN C. DISULPHIDE BRIDGES AND DIMERS OF BENCE-JONES PROTEIN. J Mol Biol. 1964 Sep;9:836–838. doi: 10.1016/s0022-2836(64)80192-3. [DOI] [PubMed] [Google Scholar]
  19. OLINS D. E., EDELMAN G. M. RECONSTITUTION OF 7S MOLECULES FROM L AND H POLYPEPTIDE CHAINS OF ANTIBODIES AND GAMMA-GLOBULINS. J Exp Med. 1964 May 1;119:789–815. doi: 10.1084/jem.119.5.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. PUTNAM F. W., EASLEY C. W., HELLING J. W. STRUCTURAL STUDY OF HUMAN GAMMA-GLOBULIN THROUGH THE ANALYSIS OF THE TRYPTIC PEPTIDES OF BENCE-JONES PROTEINS. Biochim Biophys Acta. 1963 Oct 8;78:231–233. doi: 10.1016/0006-3002(63)91632-9. [DOI] [PubMed] [Google Scholar]
  21. ROHOLT O. A., RADZIMSKI G., PRESSMAN D. POLYPEPTIDE CHAINS OF ANTIBODY: EFFECTIVE BINDING SITES REQUIRE SPECIFICITY IN COMBINATION. Science. 1965 Feb 5;147(3658):613–615. doi: 10.1126/science.147.3658.613. [DOI] [PubMed] [Google Scholar]
  22. ROHOLT O., ONOUE K., PRESSMAN D. SPECIFIC COMBINATION OF H AND L CHAINS OF RABBIT GAMMA-GLOBULINS. Proc Natl Acad Sci U S A. 1964 Feb;51:173–178. doi: 10.1073/pnas.51.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. SOBER H. A., PETERSON E. A. Protein chromatography on ion exchange cellulose. Fed Proc. 1958 Dec;17(4):1116–1126. [PubMed] [Google Scholar]
  24. TERRY W. D., FAHEY J. L. SUBCLASSES OF HUMAN GAMMA-2-GLOBULIN BASED ON DIFFERENCES IN THE HEAVY POLYPEPTIDE CHAINS. Science. 1964 Oct 16;146(3642):400–401. doi: 10.1126/science.146.3642.400. [DOI] [PubMed] [Google Scholar]

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