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. 1966 Dec;11(6):561–570.

Studies on the types of immune responses to synthetic antigens in guinea-pigs

Yehudit Stupp, F Borek, M Sela
PMCID: PMC1423823  PMID: 5954773

Abstract

The immune response in guinea-pigs to linear and multichain copolymers of two, three or four different amino acids including tyrosine, glutamic acid, alanine and lysine, was studied.

Delayed-type response was favoured in the case of preparations of relatively low molecular weight, containing only tyrosine and glutamic acid in their potential antigenic specificity determinants. Delayed sensitivity to one of these preparations was passively transferred with lymphoid cells.

Antibody response resulted from the immunization of animals with preparations of relatively high molecular weight and with a more heterogeneous chemical composition. The antibody formation was intensified and accelerated, when p-azobenzenearsonate conjugates of the polypeptides were used as antigens.

Variations in the immunizing dose had little or no effect on the nature of the reactions. A response of exclusively delayed type could not be intensified by repeated immunizations. The presence of mycobacteria in the immunizing injection was essential for the elicitation of the response to multichain, but not to linear copolymers.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asherson G. L., Stone S. H. Selective and specific inhibition of 24 hour skin reactions in the guinea-pig. I. Immune deviation: description of the phenomenon and the effect of splenectomy. Immunology. 1965 Sep;9(3):205–217. [PMC free article] [PubMed] [Google Scholar]
  2. BEN-EFRAIM S., FUCHS S., SELA M. Hypersensitivity to a synthetic polypeptide: induction of a delayed reaction. Science. 1963 Mar 22;139(3560):1222–1223. doi: 10.1126/science.139.3560.1222. [DOI] [PubMed] [Google Scholar]
  3. BEN-EFRAIM S., FUCHS S., SELA M. PASSIVE CUTANEOUS ANAPHYLAXIS IN THE GUINEA-PIG WITH RABBIT ANTIBODIES AGAINST SYNTHETIC POLYPEPTIDES. Immunology. 1964 Mar;7:142–149. [PMC free article] [PubMed] [Google Scholar]
  4. BENACERRAF B., GELL P. G. Studies on hypersensitivity. I. Delayed and Arthustype skin reactivity to protein conjugates in guinea pigs. Immunology. 1959 Jan;2(1):53–63. [PMC free article] [PubMed] [Google Scholar]
  5. Borek F., Stupp Y., Sela M. Immunogenicity and role of size: response of guinea pigs to oligotyrosine and tyrosine derivatives. Science. 1965 Nov 26;150(3700):1177–1178. doi: 10.1126/science.150.3700.1177. [DOI] [PubMed] [Google Scholar]
  6. Borek F., Stupp Y. Specificity of delayed reactions to hapten-polypeptide conjugates. Immunochemistry. 1965 Dec;2(4):323–328. doi: 10.1016/0019-2791(65)90032-7. [DOI] [PubMed] [Google Scholar]
  7. CROWLE A. J. DELAYED HYPERSENSITIVITY AND ITS ALLERGIC IMPLICATIONS. Ann Allergy. 1964 May;22:215–228. [PubMed] [Google Scholar]
  8. Dienes L., Mallory T. B. Histological Studies of Hypersensitive Reactions. Am J Pathol. 1932 Nov;8(6):689–710.3. [PMC free article] [PubMed] [Google Scholar]
  9. FEINGOLD B. F., BENJAMINI E., SHIMIZU M. INDUCTION OF DELAYED AND IMMEDIATE TYPES OF SKIN REACTIVITY IN GUINEA PIGS BY VARIATION IN DOSAGES OF ANTIGENS. Ann Allergy. 1964 Jun;22:279–291. [PubMed] [Google Scholar]
  10. GORDON J. The prevention of delayed hypersensitivity to homologous serum and transplantation antigens in guinea pigs. Immunology. 1962 Jan;5:153–160. [PMC free article] [PubMed] [Google Scholar]
  11. Hurwitz E., Fuchs S., Sela M. On the role of charge in antigen-antibody precipitin reactions. Biochim Biophys Acta. 1965 Dec 16;111(2):512–521. doi: 10.1016/0304-4165(65)90061-9. [DOI] [PubMed] [Google Scholar]
  12. KARUSH F., EISEN H. N. A theory of delayed hypersensitivity. Science. 1962 Jun 22;136(3521):1032–1039. doi: 10.1126/science.136.3521.1032. [DOI] [PubMed] [Google Scholar]
  13. LAWRENCE H. S. The delayed type of allergic inflammatory response. Am J Med. 1956 Mar;20(3):428–447. doi: 10.1016/0002-9343(56)90128-0. [DOI] [PubMed] [Google Scholar]
  14. LESKOWITZ S. Immunochemical study of antigenic specificity in delayed hypersensitivity. II. Delayed hypersensitivity to polytyrosine-azobenzenearsonate and its suppression by haptens. J Exp Med. 1963 Jun 1;117:909–923. doi: 10.1084/jem.117.6.909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MAURER P. H. (ANTIGENICITY OF POLYPEPTIDES (POLY-ALPHA-AMINO ACIDS). IV. STUDIES IN GUINEA PIGS. J Immunol. 1963 Apr;90:493–505. [PubMed] [Google Scholar]
  16. MAURER P. H., CASHMAN T. ANTIGENICITY OF POLYPEPTIDES (POLY-ALPHA-AMINO ACIDS). VIII. STUDIES IN GUINEA PIGS. J Immunol. 1963 Mar;90:393–398. [PubMed] [Google Scholar]
  17. MAURER P. H., GERULAT B. F., PINCHUCK P. ANTIGENICITY OF POLYPEPTIDES (POLY-ALPHA-AMINO ACIDS). V. STUDIES IN RABBITS. J Immunol. 1963 Mar;90:381–387. [PubMed] [Google Scholar]
  18. MAURER P. H. Immunologic studies with ethylene oxide-treated human serum. J Exp Med. 1961 Jun 1;113:1029–1039. doi: 10.1084/jem.113.6.1029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. MAYER R. L. The significance of fibroid protein carriers for the development of allergic skin reactions of the delayed type. Int Arch Allergy Appl Immunol. 1957;10(1):13–22. doi: 10.1159/000228361. [DOI] [PubMed] [Google Scholar]
  20. NELSON D. S., BOYDEN S. V. THE CUTANEOUS REACTIVITY OF GUINEA PIGS TO PURE PROTEIN ANTIGENS. I. A CRITICAL EVALUATION OF METHODS FOR THE INDUCTION OF DELAYED-TYPE HYPERSENSITIVITY TO PURE PROTEINS. Int Arch Allergy Appl Immunol. 1964;25:279–303. doi: 10.1159/000229529. [DOI] [PubMed] [Google Scholar]
  21. PAPPENHEIMER A. M., SALVIN S. B., UHR J. W. Delayed hypersensitivity. II. Induction of hypersensitivity in guinea pigs by means of antigen-antibody complexes. J Exp Med. 1957 Jan 1;105(1):11–24. doi: 10.1084/jem.105.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. SALVIN S. B. Occurrence of delayed hypersensitivity during the development of Arthus type hypersensitivity. J Exp Med. 1958 Jan 1;107(1):109–124. doi: 10.1084/jem.107.1.109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. SALVIN S. B., SMITH R. F. Delayed hypersensitivity and the anamnestic response. J Immunol. 1960 May;84:449–457. [PubMed] [Google Scholar]
  24. SELA M., FUCHS S., ARNON R. Studies on the chemical basis of the antigenicity of proteins. 5. Synthesis, characterization and immunogenicity of some multichain and linear polypeptides containing tyrosine. Biochem J. 1962 Oct;85:223–235. doi: 10.1042/bj0850223. [DOI] [PMC free article] [PubMed] [Google Scholar]

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